The invention discloses a sulfide ore flame-retardant method based on a microorganism-inhibitor comprehensive effect. The sulfide ore flame-retardant method comprises the following steps: 1, culturing acidophilic Acidithiobacillus caldus; 2, mixing the sulfide ore and the cultured bacterial liquid, adding a leaching aid, putting the mixture into a sealed container, and carrying out desulfurization treatment in a shaking table; 3, mixing a foaming agent, a foam stabilizer and a stopping agent to obtain a foam stopping agent; and 4, mixing the desulfurized sulfide ore with a foam inhibitor, and carrying out flame retardance on the sulfide ore. The sulfur content on the surface of the sulfide ore is reduced through the microbial action, surface oxidation of the sulfide ore and heat accumulation caused by oxidation heat release are reduced, so that the spontaneous combustion tendency of the sulfide ore is reduced, the sulfide ore is covered with the foam stopping agent, generated foam tightly covers the surface of the sulfide ore, and the spontaneous combustion tendency of the sulfide ore is reduced. Oxygen is isolated to interrupt the spontaneous combustion process, the spontaneous combustion tendency of the sulfide ore is reduced, and the sulfide ore spontaneous combustion prevention purpose under the comprehensive action of internal and external factors is achieved.