A kind of method for removing hydrogen sulfide in biogas by thermophilic acid thiobacillus

A technology based on Acidophilus thiobacillus and hydrogen sulfide, applied in chemical instruments and methods, methods based on microorganisms, biochemical equipment and methods, etc., can solve the problems that desulfurization stability is easily affected by ambient temperature, etc., and achieve cost reduction and The effect of high workload and high removal efficiency

Active Publication Date: 2019-08-16
NANJING TECH UNIV
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Problems solved by technology

However, its desulfurization stability is easily affected by the ambient temperature.

Method used

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  • A kind of method for removing hydrogen sulfide in biogas by thermophilic acid thiobacillus
  • A kind of method for removing hydrogen sulfide in biogas by thermophilic acid thiobacillus

Examples

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Embodiment 1

[0025] This example provides an experiment for removing hydrogen sulfide in biogas by adding a strain of mesophilic acidophilus Thiobacillus.

[0026] Step 1: Carry out recovery culture of mesophilic acidophilus Thiobacillus strain, draw 0.3~0.5mL of 9K medium with a sterile pipette, drop it into the ampoule, shake gently, so that the freeze-dried bacteria dissolve and form a suspension, absorb all The bacterial suspension was transferred to 5 mL of 9K medium, and the stirring speed was 170 r / min, and the temperature was 40-45 ° C for 48-72 hours. Since the strains were stored in freeze-drying, the lag period was long, so the cultured one generation The mesophilic acidophilus thiobacillus culture solution was inoculated into 5mL liquid medium at an inoculum volume of 3%~5%, and the same culture conditions were carried out for two consecutive subcultures, and the recovered mesophilic acidophilus thiobacillus culture solution Inoculate 3%~5% of the inoculum into 80mL liquid medi...

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Abstract

The invention discloses a method for removing hydrogen sulfide in methane by acidithiobacillus caldus. The method comprises the following steps of introducing to-be-treated methane into fermenting liquid of the acidithiobacillus caldus; removing the hydrogen sulfide in the methane by the fermenting of the acidithiobacillus caldus, wherein the fermenting temperature is 40 to 45 DEG C; the acidithiobacillus caldus is No. 1.7296 strain in China General Microbiological Culture Collection Center; the number of live cells of strain in the fermenting liquid of the acidithiobacillus caldus is 2.0*10<6> to 10.0*10<6> per mL. The method has the advantages that the operation is convenient, the technological process is simple, the utilization value of resources is improved, the cost is reduced, the desulfurizing efficiency is high, and the like; the hydrogen sulfide in the methane is stably and efficiently removed, the utilization level of the methane is favorably improved, and the desulfurizing problem of the methane is solved.

Description

technical field [0001] The invention belongs to the technical field of bioenergy, and in particular relates to a method for removing hydrogen sulfide in biogas by mesophilic acidophilus thiobacterium. Background technique [0002] With the rapid development of the economy, the demand for energy around the world is increasing day by day. In the energy structure, fossil energy such as oil and coal still occupies a dominant position. If human beings rely too much on these traditional fossil energy, their non-renewability will definitely cause an energy crisis. As a new green energy, biogas has gradually attracted people's attention. Biogas is a mixed gas, generally containing CH 4 60%~70%, CO 2 30%~40%, a small amount of H 2 S, water vapor, NH 3 Wait. Among them, hydrogen sulfide is harmful to personal safety, environment and equipment. For personal safety, hydrogen sulfide has a strong stimulating effect on mucous membranes and is a strong nerve poison. As far as the e...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C10L3/10B01D53/84B01D53/52C02F3/28C12N1/20C12R1/01C02F103/32
CPCB01D53/52B01D53/84B01D2258/05C02F3/28C02F3/2893C02F2103/325C10L3/103C12N1/20Y02A50/20Y02E50/30Y02P20/59Y02W10/10
Inventor 贾红华韩周雍晓雨周俊陈非儿周华姜岷韦萍
Owner NANJING TECH UNIV
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