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Application of acidithiobacillus caldus

A technology of rod-shaped bacteria and strains, applied in the field of application of new strains of Thiobacillus thermophilicum, can solve problems such as excessive heavy metal content and endogenous soil pollution, and achieve strong acid production capacity, high biotransformation efficiency, and growth quick effect

Active Publication Date: 2017-08-25
CENT SOUTH UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The adsorption and accumulation of heavy metals in the soil gradually lead to the heavy metal content in the soil seriously exceeding the standard, and the soil endogenous pollution is serious

Method used

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  • Application of acidithiobacillus caldus
  • Application of acidithiobacillus caldus
  • Application of acidithiobacillus caldus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1, the isolation of thermophilic acid sulfur bacillus (Acidithiobacillus caldusDX-csu)

[0030] Isolation of Acidithiobacillus caldusDX-csu Collect 10g of ore sample from the ore in Dexing, add it to a conical flask containing 100ml of sterile water and glass beads, shake it on a shaker at 170r / min for 20min, and take 1ml Add 9ml of sterile water, successively dilute 10 -5 , 10 -6 , 10 -7 , 10 -8 , 10 -9 , 10 -10 times, take 1ml of the above suspension and add it to 100ml sterilized 9K medium, repeat each concentration 3 times, place it in a 45°C incubator for 0.5d, 1d and 3d, repeat the above steps 4-5 times for bacteria for separation and purification.

Embodiment 2

[0031] Embodiment 2, identification of thermophilic acid sulfur bacillus (Acidithiobacillus caldusDX-csu)

[0032] (1) The sequencing results of the 16SrRNA gene sequence of the Acidithiobacillus caldus DX-csu strain showed that the 16SrRNA gene sequence of the Acidithiobacillus caldus DX-csu strain was shown in the sequence table, and the homology comparison was performed with the 16SrRNA gene sequence of Thermophilic acidsulfobacillus in Genbank according to the sequencing results , the results showed that Acidithiobacillus caldus DX-csu belongs to thermophilic acid sulfur bacillus.

[0033] (2) The sequencing results of the whole genome gene sequence of the Acidithiobacillus caldusDX-csu strain showed that the sequence results of the whole genome of the Acidithiobacillus caldusDX-csu strain were homologously compared with the whole gene sequence of Acidithiobacillus caldusDX- csu is a new strain.

Embodiment 3

[0034] Embodiment 3, the physiological property of thermophilic acid sulfur bacillus (Acidithiobacillus caldus)

[0035] Four different kinds of thermophilic acid sulfur bacillus (Acidithiobacillus caldus) culture medium solution pH and Eh of four strains that this laboratory screened were compared and Eh change with time; Its result shows that thermophilic acid sulfur bacillus (Acidithiobacillus caldusDX-csu) of the present invention ) pH is significantly lower than the other three, and the results show that the Eh of Acidithiobacillus caldusDX-csu of the present invention is significantly higher than the other three, indicating that the strain of the present invention has strong acid production ability. See attached figure 1 ,2.

[0036] Have compared bacterium concentration change with time in the thermophilic acid sulfur bacillus (Acidithiobacillus caldus) substratum of four different kinds; the other three. See attached image 3 .

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Abstract

The invention belongs to the technical field of treating heavy metal pollution with microorganisms, discloses application of acidithiobacillus caldus and particularly discloses application of a new bacterial strain DX-csu in the acidithiobacillus caldus. A preservation number of the bacterial strain is CGMCC No. 14008 and the bacterial strain is named acidithiobacillus caldus. The acidithiobacillus caldus disclosed by the invention has good biotransformation action to iron and manganese-bond cadmium in soil and provides technical guidance for efficient metal leaching with the microorganisms, environmental treatment and the like.

Description

technical field [0001] The invention belongs to the technical field of microbial control of heavy metal pollution, and in particular relates to the application of a new strain of thermophilic sulfur bacillus that grows rapidly and has high biotransformation efficiency for soil cadmium. Background technique [0002] Soil is an important part of the ecological environment. In various environmental elements, such as areas with serious water pollution and air pollution, along with natural phenomena such as water flow, rainfall, and atmospheric flow, pollutants will eventually enter the soil and gradually transform into soil pollution. make the soil the ultimate receptor for pollutants. The adsorption and accumulation of heavy metals in the soil gradually lead to the heavy metal content in the soil seriously exceeding the standard, and the soil endogenous pollution is serious. At present, the adverse effects caused by soil pollution have attracted much attention and become a hot...

Claims

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Application Information

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IPC IPC(8): C12N1/20B09C1/10C12R1/01
CPCB09C1/10C12N1/20C12N1/205C12R2001/01
Inventor 刘宏伟柏连阳蒋慧丹刘学端尹华群梁伊丽胡瑾
Owner CENT SOUTH UNIV
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