51results about How to "Efficient biotransformation" patented technology

The invention discloses a preparation method of compound enzymes and probiotics for forage by biotransformation of broccoli rhizome and soybean cake, including the steps of fully mixing Pu'er fermented tea, mushroom stick, agrocybe cylindracea stick, first broccoli rhizome, first soybean cake, first wheat bran, first apple slag and first water after pile fermentation and obtaining a starter after habituated culture; and fully mixing the starter, second broccoli rhizome, second soybean cake, second wheat bran, second apple slag and second water and obtaining a compound enzyme and probiotics for forage after fermentation. In the invention, safe floras at food grade with stable microbiology are used for the fermentation of broccoli rhizome and soybean cake in order to prepare compound enzymes and probiotics for forage that has every good performance index, and the biotransformation of broccoli rhizome and soybean cake has low cost and high efficiency. At the same time, in the invention, microbes are easy to grow; the enzyme activity is high; the fermentation is extensive; and no strict aseptic condition is needed, therefore the invention method is suitable for industrial mass production.

The invention provides a method for rapidly propagating earthworms and producing high-quality earthworm manure by using solid-liquid separated cow dung and manure slurry. The solid-liquid separated raw cow dung is used to establish an earthworm bed, and I completely irrigated with water before inoculation of earthworms, the manure slurry is poured to the surface of the culture bed in the breedingperiod to consume a waste liquid and increase the nutrition of the matrix, and an ecological refuge island for earthworms is established in a part of a fresh manure slurry-free area in the breeding period to make the earthworms gradually adapt to the manure slurry environment; and earthworm manure and earthworm cocoons are scraped and collected when the surface of the earthworm bed is taken by theearthworms and a large amount of the earthworm cocoons are distributed, earthworm larvae enter a lower layer and continuously produce cocoons, the collected earthworm cocoons and earthworm manure areused to cover a newly established earthworm bed, high-nutrition and high-microbial activity earthworm manure is continuously collected after the earthworm larvae hatch, and the earthworm larvae are cultured until adult earthworms are adult for sales. The method can realize recycling of intensive cattle waste liquid wastes, increase the yield of the earthworms and greatly improve the nutrient index and the microbial index of the earthworm manure, so the purposes of laborsaving mechanical collection and treatment of the cow manure, high earthworm breeding efficiency, resource utilization of nutrient elements, economic benefit maximizing, environmentally friendliness and ecologicalization are achieved.

The invention discloses a method for biocatalytic synthesis of an alkylpyrazine containing a monomethyl semi ring, which belongs to the technical field of biosynthesis. The method adopts whole cell catalysis, isotope tracer, pure enzyme catalysis, gene knockout and replenishment to determine that biosynthesis of alkylpyrazine containing monomethyl semi ring by threonine dehydrogenase (including 2,5-DMP, TMP) has important functions. The method also found that the step of catalyzing the threonine by threonine dehydrogenase TDH to generate aminoacetone is not only applicable to 2,5-DMP, but alsoto other alkylpyrazine containing the monomethyl semi ring, and the method proves that the threonine dehydrogenase TDH is involved in the biosynthesis process of 2,3,5-trimethyl containing the monomethyl semi ring.

The present invention relates to the field of biotransformation of furanic compounds. More particular the present invention relates to novel genetically modified cells with improved characteristics for biocatalytic transformation of furanic compounds and a vector suitable for the genetic modification of a host cell. Further aspects of the invention are aimed at processes for biotransformation of 5-(hydroxymethyl)furan-2-carboxylic acid (HMF-acid) and its precursors with the use of the cell according to the invention.

The invention discloses a method for preparing a compound enzyme and probiotic preparation for feed through the biotransformation of broad bean stems / leaves and cottonseed meals. The method comprises the steps of: thoroughly mixing cooked Pu'er tea, mushroom sticks, tea tree mushroom sticks, first broad bean stems / leaves, first cottonseed meals, first wheat bran, first apple pomace and first water which are subjected to pile fermentation, and carrying out acclimation culture so as to form a leavening agent; and thoroughly mixing the leavening agent, second broad bean stems / leaves, second cottonseed meals, second wheat bran, second apple pomace and second water, and carrying out fermentation, thereby obtaining the compound enzyme and probiotic preparation for feed. According to the method, the compound enzyme and probiotic preparation with better various performance indexes is prepared through fermenting the broad bean stems / leaves and the cottonseed meals by using food-grade safe flora with stable microbial states, so that the low-cost and high-efficiency biotransformation of the broad bean stems / leaves and the cottonseed meals is realized. Meanwhile, the method disclosed by the invention has the advantages that microbes grow easily, the enzyme activity is high, the fermentation process is extensive, and the industrial large-scale production is facilitated.

The invention discloses a method for preparing a compound enzyme and probiotic preparation for feed by biotransformation of reed and peanut cake. The method comprises the following steps of: fully mixing Pu'er ripe tea, shiitake mushroom log, agrocybe aegerita mushroom log, first reed, first peanut cake, first wheat bran, first apple pomace and first water which are fermented by piling, and performing acclimation culture so as to form a fermentation agent; and fully mixing the fermentation agent, second reed, second peanut cake, second wheat bran, second apple pomace and second water, and fermenting so as to obtain the compound enzyme and probiotic preparation for feed. In the method disclosed by the invention, a food-grade safe bacterial group with stable microecology is utilized for fermenting the reed and the peanut cake so as to prepare the compound enzyme and probiotic preparation for feed, which has various better performance indexes, and the low-cost and high-efficient biotransformation of the reed and the peanut cake can be realized. Simultaneously, the method disclosed by the invention has the advantages of easiness in growth of microbes, high enzyme activity, extensive fermentation process and easiness in industrial large-scale production.

The invention belongs to the technical field of treating heavy metal pollution with microorganisms, discloses application of acidithiobacillus caldus and particularly discloses application of a new bacterial strain DX-csu in the acidithiobacillus caldus. A preservation number of the bacterial strain is CGMCC No. 14008 and the bacterial strain is named acidithiobacillus caldus. The acidithiobacillus caldus disclosed by the invention has good biotransformation action to iron and manganese-bond cadmium in soil and provides technical guidance for efficient metal leaching with the microorganisms, environmental treatment and the like.

The invention discloses a method for constructing a methanol quick utilization strain. The method comprises the steps of firstly, constructing a methanol and xylose mandatory co-utilization basic strain, then performing adaptability evolution on the basic strain by using methanol and xylose as carbon sources to obtain a strain of which the growth rate is significantly accelerated, and finally strengthening the activity of downstream metabolic pathway relevant enzymes of ribulose 5 phosphate in the strain. The invention further discloses the constructed methanol bioconversion strain and a method for performing methanol bioconversion through the strain. Through the strain disclosed by the invention, the methanol and the xylose can be used as the carbon sources, even the methanol is used as the only carbon source, methanol bioconversion is performed, and the conversion efficiency of the methanol is significantly improved. The invention further discloses mutants of some enzymes in the strain, and a method for performing methanol bioconversion through the mutants.

The invention relates to a sweet potato primary pulp fermented beverage and a technological method. The technological method comprises the following steps of (1) thoroughly cleaning sweet potatoes, and soaking the cleaned sweet potatoes with a sodium hypochlorite disinfecting solution for disinfection for 20 minutes; (2) preparing a multi-functional compound solution; (3) breaking the treated sweet potatoes for the first time, adding the multi-functional compound solution, and performing filtration with a 90-120-mesh pressurized sieve so as to realize residue-pulp separation; (4) conveying sweet potato starch pulp into a standing thin layer of a clean precipitating tank, and performing natural quick precipitation on starch for 30 minutes; and (5) according to the operation, repeatedly utilizing natural precipitation or centrifuging to take primary pulp broken sweet potatoes after starch is obtained, and completing a group of processing cycles. According to the sweet potato primary pulpfermented beverage and a technological method disclosed by the invention, discharge solutions during production of sweet potato starch are in efficient bioconversion through a fermentation technique,protein and massive micro nutrients are enriched, a fresh yam primary pulp purely natural instant solid beverage is made according to a conventional beverage processing procedure, waste is turned into wealth, zero discharge of waste water during traditional starch production is realized, and economic, social and ecological benefits are considered at the same time.

The invention discloses a method for preparing a composite enzyme / probiotic preparation for feed by bioconverting watermelon vine and olive cake pulp, which comprises the following steps: thoroughly mixing Pu'er tea after pile fermentation, shiitake stick, agrocybe aegerita stick, first watermelon vine, first olive cake pulp, first wheat bran, first apple pomace and first water, and carrying out acclimatization culture to form a leavening agent; and thoroughly mixing the leavening agent, second watermelon vine, second olive cake pulp, second wheat bran, second apple pomace and second water, and fermenting to obtain the composite enzyme / probiotic preparation for feed. In the invention, the microecologically-stable food-grade safe florae are utilized to ferment the watermelon vine and olive cake pulp to prepare the composite enzyme / probiotic preparation for feed with favorable performance indexes, thereby implementing low-cost high-efficiency bioconversion of the watermelon vine and olive cake pulp. Meanwhile, the method disclosed by the invention has the advantages of easy growth of microorganisms, high enzyme activity and extensive fermentation process, and can easily implement industrialized large-scale production.

The invention discloses an endophytic fungus strain for efficient biotransformation of betulinic acid and application of the endophytic fungus strain. The invention firstly provides a Phomopsis sp. WDS2 strain, and the preservation number of the Phomopsis sp. WDS2 in China Center for Type Culture Collection is CCTCC NO: M 20199394. The invention further provides a microbial inoculum containing thePhomopsis sp. WDS2 and application of the microbial inoculum. The Phomopsis sp. WDS2 disclosed by the invention has the capability of efficiently and biologically converting betulin into betulinic acid; the betulinic acid conversion amount reaches 23.5 mg / L, so that the Phomopsis sp. WDS2 provides an excellent bacterial strain material for the development of anticancer and antiviral products in the future, and has an important value.

There is provided SHC / HAC derivatives, amino acid sequences comprising the SHC / HAC derivatives, nucleotide sequences encoding the SHC / HAC derivatives, vectors comprising nucleotide sequences encoding the SHC / HAC derivatives, recombinant host cells comprising nucleotide sequences encoding the SHC / HAC derivatives and applications of the recombinant host cells comprising either SHC / HAC derivatives or WT SHC / HAC enzymes in methods to prepare (−)-Ambrox and SHC / HAC enzymes in methods to prepare (−)-Ambrox.

The invention discloses a method for assisting whole-cell transformation to synthesize L-asparagine, which constructs a system for regenerating ATP by single-enzyme transformation of AMP by utilizingIII-class polyphosphate kinase 2, and applies the system to biosynthesis of L-asparagine. The method comprises the following steps: expressing III-type PPK2 from Deinococcus ficus in Escherichia coliRosetta (DE3), wherein the enzyme activity of the III-type PPK2 is 13.19 U.mL <-1 >, and the enzyme activity of the III-type PPK2 from Deinococcus ficus is 13.19 U.mL<-1>. E. Coli Rosetta (DE3) / pET21a-DfiPPK2-III and B. Subtilius WB600 / pMA5-LsaAS-A whole cells are used as catalysts to catalyze L-Asp to synthesize L-Asn, and the reaction conditions are optimized. Results show that the yield of L-Asn reaches 90.15%, which is 80% higher than that of sodium hexametaphosphate directly added with 180mmol. L<-1>, by supplementing sodium hexametaphosphate with the final concentration of 60mmol. L <-1>twice to wet cells of the two recombinant bacteria under optimal reaction conditions.

The invention discloses recombinant bacteria synthesizing 2,5-dimethylpyrazine (2,5-DMP) by catalysis, and belongs to the technical field of biosynthesis. A strain with high yield of the 2,5-DMP of L-threonine can be used to perform recombinant expression of threonine dehydrogenase (TDH). Preferably, when recombinant bacillus subtilis co-expressing NOX and the E.coli-derived TDH is fermented for 24 h with 5.83 g / L L-threonine as a substrate, the yield can reach 616.04 mg / L, the production intensity can reach 25.67 mg / (L.h), and the conversion rate can reach 10.6%; compared with a wild-type strain, the strain is increased by 22.5 times in yield, the production intensity and conversion rate are greatly improved, and efficient biotransformation of the 2,5-DMP is realized.

The invention discloses a method for preparing a composite enzyme / probiotic preparation for feed by bioconverting pumpkin vine and leaf and olive cake pulp, which comprises the following steps: thoroughly mixing Pu'er tea after pile fermentation, shiitake stick, agrocybe aegerita stick, first pumpkin vine, first olive cake pulp, first wheat bran, first apple pomace and first water, and carrying out acclimatization culture to form a leavening agent; and thoroughly mixing the leavening agent, second pumpkin vine, second olive cake pulp, second wheat bran, second apple pomace and second water, and fermenting to obtain the composite enzyme / probiotic preparation for feed. In the invention, the microecologically-stable food-grade safe florae are utilized to ferment the pumpkin vine and olive cake pulp to prepare the composite enzyme / probiotic preparation for feed with favorable performance indexes, thereby implementing low-cost high-efficiency bioconversion of the pumpkin vine and olive cake pulp. Meanwhile, the method disclosed by the invention has the advantages of easy growth of microorganisms, high enzyme activity and extensive fermentation process, and can easily implement industrialized large-scale production.

The invention relates to a special controlled release fertilizer for selenium-rich sweet potatoes and a preparation method thereof. The special controlled release fertilizer for selenium-rich sweet potatoes comprises core fertilizers and a coating layer, wherein the core fertilizers include nitrogen fertilizers, phosphorus fertilizers, potassium fertilizers and selenium fertilizers; the total content of nitrogen phosphorus and potassium nutrients is 30-50%; the selenium fertilizers account for 0.4-0.9% of the total core fertilizers by weight; the weight ratio of N to P2O5 to K2O is (1-2.5) to (0.6-1) to (2-3.5); the coating layer comprises polycaprolactone and an additive starch. The invention also provides a preparation method of the special controlled release fertilizer for selenium-rich sweet potatoes. The fertilizer is applied to the bottoms of planting furrows once before sweet potato transplantation and top application is unnecessary. The application amount of the fertilizer per mu is 40-60kg. The special controlled release fertilizer for producing selenium-rich sweet potatoes has the effects that the nutrients of the fertilizer meet the requirements of the whole growing periods of the sweet potatoes and have controlled release effects; fixation of selenium by soil can be reduced and the effective period can be prolonged.

The invention discloses a preparation method of pollen pini eggs. The preparation method comprises the following steps of: adding pollen pini to a feed for laying hens to obtain a mixed feed, and feeding the laying hens with the mixed feed. In the preparation method of the pollen pini eggs, the feed with the pollen pini is fed to the laying hens, efficient biotransformation is realized by taking the laying hens as carriers to ensure that the essence of the pollen pini is absorbed in eggs, and therefore, the pollen pini eggs with multiple health care and treatment effects are prepared. The quality of the eggs is improved, and the additive values of the eggs increase.

The invention discloses a method for preparing a composite enzyme / probiotic preparation for feed by bioconverting soybean stem and leaf and tea seed cake pulp, which comprises the following steps: thoroughly mixing Pu'er tea after pile fermentation, shiitake stick, agrocybe aegerita stick, first soybean stem and leaf, first tea seed cake pulp, first wheat bran, first apple pomace and first water, and carrying out acclimatization culture to form a leavening agent; and thoroughly mixing the leavening agent, second soybean stem and leaf, second tea seed cake pulp, second wheat bran, second apple pomace and second water, and fermenting to obtain the composite enzyme / probiotic preparation for feed. In the invention, the microecologically-stable food-grade safe florae are utilized to ferment the soybean stem and leaf and tea seed cake pulp to prepare the composite enzyme / probiotic preparation for feed with favorable performance indexes, thereby implementing low-cost high-efficiency bioconversion of the soybean stem and leaf and tea seed cake pulp. Meanwhile, the method disclosed by the invention has the advantages of easy growth of microorganisms, high enzyme activity and extensive fermentation process, and can easily implement industrialized large-scale production.

The present invention relates to an improved biocatalytic process for producing vanillin from ferulic acid based on a genetically engineered Pseudomonas strains, as well as to said Pseudemonas strains.

The invention discloses a preparation method of a compound enzyme and probiotic preparation for a feed through biotransformation of a potato stem leaf and a sunflower cake. The method comprises the following steps: fully mixing Pu-Er ripe tea having undergone pile fermentation, mushroom stick, agrocybe cylindracea stick, a first potato stem leaf, a first sunflower cake, first wheat bran, first apple pomace and first water and carrying out domestication and cultivation so as to obtain a leavening agent; and fully mixing the leavening agent, a second potato stem leaf, a second sunflower cake, second wheat bran, second apple pomace and second water and carrying out fermentation so as to obtain the compound enzyme and probiotic preparation for a feed. According to the invention, microecologically stable food-grade safe floras are used for fermentation of the potato stem leaf and the sunflower cake so as to prepare the compound enzyme and probiotic preparation with good performance indexes for the feed, and low-cost high-efficiency biotransformation of the potato stem leaf and the sunflower cake is realized. Meanwhile, the method provided by the invention has the advantages of easy growth of microbes, high enzyme activity, extensive fermentation process and easy realization of industrial large-scale production.

The invention discloses a method for preparing a composite enzyme / probiotic preparation for feed by bioconverting rice straw and castor cake pulp, which comprises the following steps: thoroughly mixing Pu'er tea after pile fermentation, shiitake stick, agrocybe aegerita stick, first rice straw, first castor cake pulp, first wheat bran, first apple pomace and first water, and carrying out acclimatization culture to form a leavening agent; and thoroughly mixing the leavening agent, second rice straw, second castor cake pulp, second wheat bran, second apple pomace and second water, and fermenting to obtain the composite enzyme / probiotic preparation for feed. In the invention, the microecologically-stable food-grade safe florae are utilized to ferment the rice straw and castor cake pulp to prepare the composite enzyme / probiotic preparation for feed with favorable performance indexes, thereby implementing low-cost high-efficiency bioconversion of the rice straw and castor cake pulp. Meanwhile, the method disclosed by the invention has the advantages of easy growth of microorganisms, high enzyme activity and extensive fermentation process, and can easily implement industrialized large-scale production.

The invention discloses a biogas purification double-membrane aeration membrane bio-membrane reactor and a use method thereof, and relates to the technical field of renewable energy sources. The invention aims to solve the problem of insufficient bioconversion of carbon dioxide and hydrogen due to solubility difference of the carbon dioxide and the hydrogen in a biogas biological purification process. According to the method, the biogas purification double-membrane aeration membrane bio-membrane reactor realizes high-efficiency biological conversion of hydrogen and carbon dioxide by controlling the partial pressure of the hydrogen and the biogas according to different solubility of the hydrogen and the carbon dioxide, and the conversion rate is higher than 93%. By adopting a bubble-free aeration mode, microorganisms taking H2 / CO2 as electron donors can be attached to the surface of the hollow bubble-free fiber membrane, the H2 / CO2 biological conversion rate is effectively improved, and the yield of liquid chemical acetic acid can reach 45.36 mmol / L / d while the purity of biogas reaches 96% or above. The invention can obtain the biogas purification double-membrane aeration membrane bio-membrane reactor and the use method thereof.

The invention discloses a method for preparing a composite enzyme / probiotic preparation for feed by bioconverting pumpkin vine and leaf and olive cake pulp, which comprises the following steps: thoroughly mixing Pu'er tea after pile fermentation, shiitake stick, agrocybe aegerita stick, first pumpkin vine, first olive cake pulp, first wheat bran, first apple pomace and first water, and carrying out acclimatization culture to form a leavening agent; and thoroughly mixing the leavening agent, second pumpkin vine, second olive cake pulp, second wheat bran, second apple pomace and second water, and fermenting to obtain the composite enzyme / probiotic preparation for feed. In the invention, the microecologically-stable food-grade safe florae are utilized to ferment the pumpkin vine and olive cake pulp to prepare the composite enzyme / probiotic preparation for feed with favorable performance indexes, thereby implementing low-cost high-efficiency bioconversion of the pumpkin vine and olive cake pulp. Meanwhile, the method disclosed by the invention has the advantages of easy growth of microorganisms, high enzyme activity and extensive fermentation process, and can easily implement industrialized large-scale production.

The invention discloses a method for preparing a compound enzyme and probiotic preparation for feed by biotransformation of luffa stem and castor cake. The method comprises the following steps of: fully mixing Pu'er ripe tea, shiitake mushroom log, agrocybe aegerita mushroom log, first luffa stem, first castor cake, first wheat bran, first apple pomace and first water which are fermented by piling, and performing acclimation culture so as to form a fermentation agent; and fully mixing the fermentation agent, second luffa stem, second castor cake, second wheat bran, second apple pomace and second water, and fermenting so as to obtain the compound enzyme and probiotic preparation for feed. In the method disclosed by the invention, a food-grade safe bacterial group with stable microecology is utilized for fermenting the luffa stem and the castor cake so as to prepare the compound enzyme and probiotic preparation for feed, which has various better performance indexes, and the low-cost and high-efficient biotransformation of the luffa stem and the castor cake can be realized. Simultaneously, the method disclosed by the invention has the advantages of easiness in growth of microbes, high enzyme activity, extensive fermentation process and easiness in industrial large-scale production.

The invention discloses a method for combined production of bioethanol and pullulan from lignocellulose substances. The method comprises the following steps: subjecting the lignocellulose substances to alkaline-process pretreatment and xylanase enzymatic hydrolysis and then carrying out solid-liquid separation so as to respectively obtain a hemicellulose hydrolysate (xylose liquid) and a solid-phase residue; subjecting the solid-phase residue to lignin extraction so as to obtain a cellulose residue; directly applying the xylose liquid to fermentation production of pullulan (wherein yield is no less than 0.29 g of pullulan per g of xylose) and subjecting the cellulose residue to synchronous saccharification and fermentation to produce ethanol; subjecting fermentation broth containing pullulan to fermentation and distillation with fiber residue, carrying out alcohol precipitation on obtained ethanol, carrying out extraction and refining so as to obtain a pullulan product, combining ethanol-containing waste liquid produced during purifying with cellulose residue ethanol fermentation broth and carrying out distillation so as to realize recovery and reuse of ethanol. The method can realize full utilization and high-value conversion of hemicellulose and cellulose, saves resources and achieves the purposes of environmental protection and economic benefit improvement.

The present invention relates to an improved biocatalytic process for producing vanillin from ferulic acid based on a genetically engineered Pseudomonas strains, as well as to said Pseudemonas strains.