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Intermediate-temperature mineral leaching composite microbial system for leaching copper pyrites

A technology of compound bacteria and chalcopyrite, applied in the directions of microorganism-based methods, bacteria, and process efficiency improvement, can solve the problems of low oxygen and carbon dioxide content, negative effects of leaching efficiency, limited gas-liquid transmission, etc. Low temperature, good environmental adaptability, easy to cultivate

Inactive Publication Date: 2014-08-27
有研资源环境技术研究院(北京)有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, thermoacidophilic bacteria are extremely sensitive to high pulp concentration, gas-liquid transmission is limited at high temperature, and the content of oxygen and carbon dioxide in the leaching solution is low, which has a negative impact on the leaching efficiency

Method used

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  • Intermediate-temperature mineral leaching composite microbial system for leaching copper pyrites
  • Intermediate-temperature mineral leaching composite microbial system for leaching copper pyrites
  • Intermediate-temperature mineral leaching composite microbial system for leaching copper pyrites

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Example 1: Obtaining of the compound bacterial strain 6Y-1 in medium-temperature ore leaching

[0018] 1. Enrichment:

[0019] Take 200mL of ordinary tap water, adjust its pH to 1.7, add 0.20g of yeast powder, add 300g of ore samples from the abandoned heap leaching pile of Dexing Copper Mine, and incubate at 55°C for 24 hours.

[0020] 2. Cultivate:

[0021] Prepare 100mL medium A according to the following formula: K 2 HPO 4 1g, (NH 4 ) 2 CO 3 2g, NaHCO 3 0.5g, CaCl 2 0.2g, MgSO 4 0.8g, 1L of distilled water; adjust the pH to 1.6-1.7. Configure medium B according to the following preparation method: add 10 g of chalcopyrite ore powder to 100 mL of ordinary tap water, preferably, the particle size of chalcopyrite ore powder is 200 mesh, and adjust the pH with concentrated sulfuric acid for acid balance until the pH Stable at 1.6-1.7. Mix media A and B before inoculation. When inoculating, first add 0.1-0.15% yeast powder to the mixed medium, and then a...

Embodiment 2

[0024] Example 2: Analysis of bacterial population composition in the compound bacterial system L-A of moderate temperature leaching

[0025] 1. Extraction of total DNA

[0026] Inoculate the composite bacterial strain obtained in Example 1 into the above-mentioned mixed medium, the inoculum amount is 5% by volume, and culture at 40-50° C. for 20 hours.

[0027] Put 1 mL of the culture solution into a 1.5 mL Eppendorf tube, centrifuge at 12000 rpm at 4°C for 5 minutes to collect the bacteria, and wash the bacteria 2-3 times with 1×TES. Re-suspend the bacteria in 435 μL TES to break up the bacteria, add 125 μL of 20 mg / mL lysozyme, 2.5 μL of 10 mg / mL proteinase K, keep warm in a 37°C water bath for 4 hours, add 50 μL of 20% SDS, and incubate at 37°C Keep warm in a water bath for 30 minutes with light vibration, add 5mol / L NaClO 4 125 μL was extracted 3-4 times with an equal volume of phenol-chloroform-isoamyl alcohol (the volume ratio of the three was 25:24:1) until no prote...

Embodiment 3

[0057] Example 3: Analysis of the leaching effect of the medium-temperature ore-leaching composite bacterial system on chalcopyrite

[0058] First, the composite bacterial strain Sulfobacillus thermosulfidooxidans 6Y-1 preserved at low temperature is activated in an Erlenmeyer flask with 200mL medium; the medium is formulated according to the following formula: K 2 HPO 4 1g, (NH 4 ) 2 CO 3 2g, NaHCO 3 0.5g, CaCl 2 0.2g, MgSO 4 0.8g, yeast powder 1g, distilled water 1L. After the activated strains were centrifuged, transfer the sludge into 200mL of acid-balanced ordinary tap water containing 5% chalcopyrite ore powder. After culturing at 55°C for one week, take 50mL of the leachate, filter and detect the copper and Iron ion concentration, calculate the leaching efficiency of copper and iron. The results showed that the leaching efficiency of copper was 34.78%, and that of iron was 30.3%. This leaching efficiency is higher than that of existing mesophilic strains. ...

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Abstract

The invention provides an intermediate-temperature mineral leaching composite microbial system for leaching copper pyrites. The composite microbial system is named Sulfobacillus thermosulfidooxidans 6Y-1, is preserved in the China center for type culture collection (CCTCC) in Wuhan University in November 10th 2010, and has a preservation number of CCTCC M2010297. The composite microbial system consists of 77% of Sulfobacillus thermosulfidooxidans, 5% of Acidithiomicrobium sp, 12% of Acidithiobacillus caldus and 6% of Ferroplasma sp. The microbial system has high copper pyrite leaching efficiency.

Description

technical field [0001] The invention relates to a compound bacterial system for leaching chalcopyrite at medium temperature. Background technique [0002] Copper is an indispensable and important metal raw material in the development of modern industry. With economic development and social progress, the status of the copper industry in the development of the national economy is increasing day by day. At present, the ambient temperature bioheap leaching technology of secondary copper sulfide ores has been widely used in commercial production. However, studies have shown that normal temperature bioleaching is difficult for chalcopyrite, the main body of copper resources. Since 1999, two major mining companies, BHP Billiton and Mintek / Bactech, have successively released the experimental results on the agitation and leaching of chalcopyrite thermophilic bacteria. The results show that the stirring leaching of chalcopyrite concentrate by extreme thermophilic acidophilus bacter...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/00C12N1/20C22B3/18C12R1/01C22B15/00
CPCY02P10/20
Inventor 刘文彦陈勃伟刘兴宇
Owner 有研资源环境技术研究院(北京)有限公司
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