Medium-temperature ore-leaching composite microbial system used for leaching of chalcopyrite and ore leaching process

A technology of compound bacteria and chalcopyrite, which is applied to the improvement of process efficiency, microorganism-based methods, bacteria, etc., to achieve the effects of convenient operation, strong commercial application prospects, and good environmental adaptability

Inactive Publication Date: 2016-07-27
有研资源环境技术研究院(北京)有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the prior art, there are few studies on the leaching of chalcopyrite by mesophilic microorganisms

Method used

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  • Medium-temperature ore-leaching composite microbial system used for leaching of chalcopyrite and ore leaching process
  • Medium-temperature ore-leaching composite microbial system used for leaching of chalcopyrite and ore leaching process
  • Medium-temperature ore-leaching composite microbial system used for leaching of chalcopyrite and ore leaching process

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Embodiment 1: the acquisition of compound bacterial strain 0Y in middle temperature leaching ore

[0014] 1. Enrichment:

[0015] Take 200mL of ordinary tap water and use H 2 SO 4 Adjust its pH to 1.7, add 0.20 g of yeast powder, add 300 g of ore samples from the abandoned heap leaching pile of Dexing Copper Mine, and keep warm at 45° C. for 24 hours to obtain an enriched culture.

[0016] 2. Cultivate:

[0017] Prepare medium A according to the following formula: K 2 HPO 4 1g, (NH 4 ) 2 CO 3 2g, NaHCO 3 0.5g, CaCl 2 0.2g, MgSO 4 0.8g, 1L of distilled water; adjust the pH to 1.6-1.7. Configure medium B according to the following preparation method: add 10 g of chalcopyrite ore powder (Dexing Copper Mine, Jiangxi Province) to 100 mL of ordinary tap water, the particle size of chalcopyrite ore powder is 200 mesh, adjust pH with concentrated sulfuric acid for acidification Equilibrate until the pH stabilizes at 1.6-1.7. Mix 100 mL of medium A with medium B bef...

Embodiment 2

[0020] Embodiment 2: Analysis of bacterial population composition in the composite bacterial system 0Y of intermediate temperature leaching ore

[0021] 1. Extraction of total DNA

[0022] Inoculate the composite bacterial strain obtained in Example 1 into medium A, the inoculum amount is 5% by volume, and cultivate at 40-50° C. for 20 hours.

[0023] Take 1 mL of the above-mentioned 20-hour culture solution and put it into a 1.5 mL Eppendorf tube, centrifuge at 12000 rpm at 4°C for 5 minutes to collect the bacteria, and wash the bacteria 2-3 times with 1×TES. Re-suspend the bacteria in 435 μLTES to break up the bacteria, add 125 μL of 20 mg / mL lysozyme, 2.5 μL of 10 mg / mL proteinase K, keep warm in a 37°C water bath for 4 hours, add 50 μL of 20% SDS, and gently shake in a 37°C water bath. Shake and keep warm for 30 minutes, add 5mol / L NaClO 4 125 μL was extracted with an equal volume of phenol-chloroform-isoamyl alcohol (volume ratio 25:24:1) for 3-4 times until no protein ...

Embodiment 3

[0054] Example 3: Analysis of the leaching effect of the compound bacterial system 0Y and JQ-3Y on chalcopyrite at medium temperature leaching

[0055] The JQ-3Y bacterial strain is another kind of chalcopyrite mesothermal leaching composite bacterial strain cultivated and domesticated in this laboratory, consisting of Acidithiomicrobium ferrooxidans (54%); acidophilic sulfur bacteria cidithiobacillus caldus (21%); Acidithiomicrobium sp (10%); iron-oxidizing acidophilic archaea Ferroplasmasp (15%).

[0056] First, the composite bacterial strains Acidithiobacillus caldus OY and JQ-3Y preserved at low temperature are activated in a Erlenmeyer flask with 200mL medium; the medium is prepared according to the following formula: K 2 HPO 4 1g, (NH 4 ) 2 CO 3 2g, NaHCO 3 0.5g, CaCl 2 0.2g, MgSO 4 0.8g, yeast powder 0.1g, distilled water 1L. Activation and culture at 140rpm at 45°C for 3 days, the concentration of the activated bacteria reached 10 7 ~10 8 cfu / mL, after centri...

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Abstract

The invention provides a medium-temperature ore-leaching composite microbial system used for leaching of chalcopyrite and an ore leaching process. The medium-temperature ore-leaching composite microbial system is Acidithiobacillus caldus 0Y which has been preserved in China Center for Type Culture Collection (CCTCC), Wuhan University, China, on January 20th, 2011, with an accession number of CCTCC No. M2010356. The medium-temperature ore-leaching composite microbial system is composed of Acidithiomicrobium ferrooxidans (81%), Acidithiobacillus caldus (13%) and Ferroplasma sp (6%). The composite microbial system has high leaching efficiency and especially has good commercial application prospects in dump leaching processes for low-grade copper sulphide like chalcopyrite.

Description

technical field [0001] The invention relates to the technical field of microbial leaching, in particular to a compound bacterial system for leaching chalcopyrite at a medium temperature and an ore leaching process thereof. Background technique [0002] With economic development and social progress, the status of the copper industry in the development of the national economy is increasing day by day. Chalcopyrite is the main body of copper resources. However, studies have shown that the bioleaching of chalcopyrite by normal temperature organisms is difficult. In addition, although some mining companies have continuously released experiments on the stirring and leaching of chalcopyrite thermophilic bacteria, it is difficult to efficiently solve the problem of chalcopyrite leaching because thermoacidophilic bacteria are extremely sensitive to high pulp concentrations. [0003] Therefore, it is a beneficial attempt to screen and cultivate efficient and highly adaptable mesophil...

Claims

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Application Information

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IPC IPC(8): C12N1/20C22B3/18C12R1/01
CPCY02P10/20
Inventor 刘文彦刘兴宇陈勃伟孟春瑜
Owner 有研资源环境技术研究院(北京)有限公司
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