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Microorganism synchronously removing ammonia and sulfureted hydrogen foul gas and method for preparing the same

A malodorous gas and microorganism technology, applied in the field of microorganisms, can solve difficult and difficult problems such as NH3 removal efficiency, and achieve the effects of easy use, simple composition and culture conditions, and simple operation

Inactive Publication Date: 2009-11-18
NINGBO UNIVERSITY OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to differences in the suitable growth factors (such as pH, nutritional requirements) of different microorganisms, it is difficult to remove H 2 S and except NH 3 The microorganisms are under suitable environmental conditions at the same time, so it is difficult to maintain a high H at the same time 2 S and NH 3 Removal efficiency

Method used

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  • Microorganism synchronously removing ammonia and sulfureted hydrogen foul gas and method for preparing the same
  • Microorganism synchronously removing ammonia and sulfureted hydrogen foul gas and method for preparing the same
  • Microorganism synchronously removing ammonia and sulfureted hydrogen foul gas and method for preparing the same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1 Study on Growth Temperature of Acidophilus Thiobacillus Thiooxidans CCW-Y2 Strain

[0036] Use SOB medium, pH = 3.0, culture at a temperature range of 15-55° C., and the test is carried out on a shaker with temperature control capability in a temperature control room. The amount of sulfate ion formed by sulfur oxidation and the pH change in the culture solution were used to indirectly calculate the oxidation of elemental sulfur and the growth status of bacteria. The growth results after 5 days of cultivation are shown in Table 1.

[0037] Table 1 The growth of CCW-Y2 strain at different temperatures

[0038] Culture temperature (℃)

[0039] It can be seen from the data in Table 1 that the optimal temperature for the growth of CCW-Y2 strain is 28-30°C, and it is a kind of mesophilic bacteria. It can still grow below 15°C, but hardly grows above 55°C.

Embodiment 2

[0040] Example 2 Study on Growth pH of Acidophilus Thiobacillus Thiooxidans CCW-Y2 Strain

[0041] The SOB culture medium is used, the temperature is 30°C, and the culture is carried out in the range of pH=0.5-5.5, and the experiment is carried out on a shaker with temperature control capability in a temperature control room. The amount of sulfate ion formed by sulfur oxidation and the pH change in the culture solution were used to indirectly calculate the oxidation of elemental sulfur and the growth state of bacteria. The growth results after 5 days of cultivation are shown in Table 2.

[0042] Table 2 The growth of CCW-Y2 strain at different pH

[0043] Medium pH

[0044] The data in Table 2 show that the CCW-Y2 strain can grow at a pH of 0.5 to 5.5, and the optimum pH for growth is 2.0 to 3.0. It is an acidophilic bacteria.

Embodiment 3

[0045] Example 3 Research on Ammonium Sulfate Resistance of Acidophilus Thiobacillus Thiooxidans CCW-Y2 Strain

[0046] A series of SOB medium with different contents of ammonium sulfate were used to cultivate at a temperature of 30°C and pH=2.5, and the experiment was carried out on a shaker with temperature control capability in a temperature control room. The specific sulfur oxidation rate (the amount of sulfur oxidation per unit mass of DCW per unit time) was used to investigate the oxidation of elemental sulfur and the growth status of bacteria. The growth results after 5 days of culture are shown in Table 3.

[0047] Table 3 The growth of CCW-Y2 strain under different ammonium sulfate concentrations

[0048] Ammonium sulfate concentration (g / L)

[0049] It can be seen from the data in Table 3 that when the concentration of ammonium sulfate rises from 0.5g / L to 10g / L, the specific sulfur oxidation rate increases accordingly. But when the concentration of ammoni...

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Abstract

A microorganism which can remove the malodorous gas of NH3 and H2S synchronously and a preparation method thereof relate to the microorganism technical field and in particular to an efficient deodorization microorganism of the mixed malodorous gas in a sewage collecting and disposing establishment and the preparation method thereof. An acidithiobacillus thiooxidans CCW-Y2 strain provided by the present invention is conserved in the General Microorganism Center of China Committee for Culture Collections of Microorganisms, and the conversing serial number CGMCC No. is 2158. The strain is ammonium sulfate tolerant type and is obtained by sieving and separating from the activated sludge of the living sewage disposal factory. The culture conditions of the acidithiobacillus thiooxidans CCW-Y2 strain of the present invention are as follows: firstly, SOB culture medium is adopted, and the components of the culture medium are the dipotassium hydrogen phosphate of 2g / L, the potassium dihydrogen phosphate of 2g / L, the magnesium chloride (MgCl2 / 7H2O) of 0.2g / L, the ammonium sulfate of 10g / L, the ferrous sulfate (FeSO4 / 7H2O) of 0.01g / L and the powdered sulfur of 10g / L; secondly, the culture condition is the temperature of 28 DEG C to 32 DEG C and 180 circles per minute; thirdly, a carrier is water, and dilute sulfuric acid (or hydrochloric acid) is added for adjusting pH value to be 2.0 to 3.0. The acidithiobacillus thiooxidans CCW-Y2 strain of the present invention can be directly inoculated into a waste gas disposing establishment (such as a biological filtration tower) and can synchronously remove the mixed malodorous gas of the H2S and the NH3 efficiently, which simplifies the operation condition of the waste gas disposing establishment and ensures that the operation is more simple.

Description

technical field [0001] The invention provides a method for simultaneously and efficiently removing ammonia (NH 3 ) and hydrogen sulfide (H 2 S) Microorganisms for malodorous gases and preparation methods thereof, relating to the field of microbiological technology, in particular to highly efficient deodorizing microorganisms for mixed malodorous gases in sewage collection and treatment facilities and preparation methods thereof. Background technique [0002] With the development of the economy and the improvement of people's living standards, stench, as a major environmental hazard, has attracted more and more attention. NH 3 and H 2 S is the most common malodorous substance and is the main exhaust gas component widely present in industrial production processes (such as sewage treatment plants, feces treatment plants, food processing plants, leather industry, animal husbandry, and composting plants, etc.). Not only toxic, but also corrosive, it is an irritating odorous s...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20B01D53/52B01D53/58B01D53/84
CPCY02A50/20
Inventor 叶芬霞
Owner NINGBO UNIVERSITY OF TECHNOLOGY
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