Mining waste containing pyrite, in the culture of iron-oxidizing and sulfur-oxidizing microorganisms

A technology of sulfur oxidizing microorganisms and pyrite, which is applied in the field of natural microorganisms, can solve the problems of increasing impurities, not knowing that pyrite is effectively used as an energy source for biomass growth, and being impractical, and achieving the effect of high production capacity

Inactive Publication Date: 2008-06-18
BIOSIGMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, this approach is not practical for economical reasons, most importantly if large-scale biomass production is to be dealt with
This problem is generally solved by using technical-industrial grade reagents, which reduces the cost of the medium, but increases potential sources of contamination in addition to impurities that may inhibit microbial growth
[0013] Thus, to the best of our knowledge, there are still no low-cost media that enable viable large-scale production of microorganisms that can be used for bioleaching; process

Method used

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  • Mining waste containing pyrite, in the culture of iron-oxidizing and sulfur-oxidizing microorganisms
  • Mining waste containing pyrite, in the culture of iron-oxidizing and sulfur-oxidizing microorganisms

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] In order to determine the growth kinetics and biomass production of combinations of Wenelen DSM 16786 and Licanantay DSM 17318 microorganisms, experiments were carried out using the following protocol using a medium modified by the incorporation of sweep tailings:

[0055] Program

[0056] Bacterial growth occurs at 6m 3 effective volume of the reactor.

[0057] A medium for the propagation of microorganisms was prepared by suspending the scavenging tailings (at 1.25% pulp concentration) in a nutrient solution consisting of: 75 g FeSO 4 / L, 0.99g (NH 4 ) 2 SO 4 / L, 0.128g NaH 2 PO 4 ·H 2 O / L, 0.0525g KH 2 PO 4 / L, 0.1gMgSO 4 ·7H 2 O / L, 0.021g CaCl 2 / L. Adjust the pH of the medium to 1.8.

[0058] To start the culture, 5,400 L of medium was mixed with 600 L of bacterial inoculum with Wenelen DSM 16786 and Licanantay DSM 17318 microorganisms.

[0059] In order to allow microorganisms to grow in the reactor, provide enriched 0.5% CO 2 air. The temperature...

Embodiment 2

[0064] In order to demonstrate that a combination of Wenelen DSM 16786 and Licanantay DSM 17318 microorganisms can be effectively continuously propagated using a medium modified by the incorporation of sweep tailings, the following protocol was used.

[0065] Program

[0066] Bacterial growth occurs at 50m 3 in industrial reactors.

[0067] A medium for microbial propagation was prepared by suspending the scavenging tailings (at 0.125% slime concentration) in a nutrient solution consisting of: 8 g FeSO 4 / L, 0.99g (NH 4 ) 2 SO 4 / L, 0.128g NaH 2 PO 4 ·H 2 O / L, 0.0525g KH 2 PO 4 / L, 0.1gMgSO 4 ·7H 2 O / L, 0.021g CaCl 2 / L. Adjust the pH of the medium to 1.8.

[0068] To start culturing, place 44m 3 The culture medium with Wenelen DSM 16786 and Licanantay DSM 17318 microorganisms 6m 3 The bacterial inoculum was mixed.

[0069] In order to allow microorganisms to grow in the reactor, provide enriched 0.5% CO 2 air. The temperature of the reactor was controlled a...

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Abstract

The invention discloses the use of mining products and sub-products that contain pyrite, such as copper concentrates, and waste from the process in which these concentrates are obtained, known as scavenger tail, as an energy source for the large-scale culture of an association of microorganisms that are useful for ore bioleaching, and that includes both isolated microorganisms, and native microorganisms from the worked ores. In particular, the invention publishes the use of mining waste known as scavenger tail from the flotation process, in the culture of an association of isolated microorganisms of the Acidithiobacillus ferrooxidans y Acidithiobacillus thiooxidans type together, with or without other native microorganisms from the worked ores.

Description

technical field [0001] The present invention relates to mining products and sub-products (such as copper concentrates) containing pyrite and wastes from the process in which these concentrates are obtained (known as "scavenger tails") ), as an energy source for the large-scale cultivation of assemblies of microorganisms that can be used for ore bioleaching, and which include both isolated microorganisms and naturally occurring microorganisms in processed ores. In particular, the present invention relates to the use of mining waste obtained from the flotation process (referred to as "sweep tailings") in the cultivation of combinations of isolated microorganisms of the Acidithiobacillus ferrooxidans and Acidithiobacillus thiooxidans types, with or without Other naturally occurring microorganisms that contain the ore being processed. Background technique [0002] In general, in the cultivation of microorganisms, artificial or specially formulated media are used, often starting...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/00B09B3/00
CPCC12N1/20C12P39/00C22B3/18C22B15/0063Y02P10/20
Inventor C·P·A·莫拉莱斯I·L·M·帕迪利亚O·R·巴迪利亚
Owner BIOSIGMA
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