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Method for extracting francisella native from bykomycin fermentation liquor

A technology of framectin sulfate and neomycin sulfate, which is applied in the direction of fermentation, antibacterial drugs, amino sugar, etc., can solve the problems of high organic solvent residue, large consumption of raw materials, and low analysis yield, so as to improve product quality , Low production cost, and the effect of reducing environmental pollution

Inactive Publication Date: 2009-01-14
SHIJIAZHUANG HUASHU PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are many shortcomings in the above method, for example, the resin type used in the past has small adsorption capacity, low analysis yield, large consumption of raw materials, and high cost
Methanol is used in the crystallization process, which is costly, pollutes the environment, and has high residues of organic solvents in the product

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] The neomycin sulfate fermentation broth is adsorbed by a strong acid cation resin, rinsed with water to remove impurities, and analyzed with 0.2M ammonia water. The analytical solution is concentrated and decolorized. The total unit of the neomycin concentrate prepared is 190-200,000 units / Milliliter, adjust the pH value to 5-9, add neomycin sulfate concentrate to each column with a mass volume ratio of 10%, and add it to the top of the weakly acidic cation resin treatment column, open the liquid outlet valve, and control the flow rate to about 50ml / min, after the neomycin sulfate concentrate is adsorbed by weak acid cations, neomycin C sulfate is eluted with 0.1M aqueous ammonia solution to remove impurities. The flow rate is controlled at 1 / 500-1 / 300 resin volume per minute. After the impurities are eluted, continue to elute neomycin C sulfate with 0.1M aqueous ammonia solution, and monitor the optical rotation of the solution at any time. When the optical rotation reac...

Embodiment 2

[0025] The neomycin sulfate fermentation broth is adsorbed by a strong acid cation resin, rinsed with water to remove impurities, analyzed with 0.2M ammonia water, the analytical solution is concentrated, and the total unit of the neomycin concentrate prepared is 190-200,000 units / ml. Adjust the PH value to 5-9, add neomycin sulfate concentrate to each column with a mass volume ratio of 10% and add it to the top of the weakly acidic cationic resin treatment column, open the liquid outlet valve, and control the flow rate to about 50ml / min. After the neomycin concentrate is adsorbed by weakly acidic cations, it is eluted with 0.5M ammonia solution to remove impurities. The flow rate is controlled at 1 / 500-1 / 300 resin volume per minute. After the impurities are eluted, continue to elute neomycin C with 0.5M aqueous ammonia solution, and monitor the optical rotation of the solution at any time. When the optical rotation reaches the highest point and begins to decrease, use an ion chro...

Embodiment 3

[0028] The neomycin fermentation broth is adsorbed by strong acid cationic resin, rinsed with water to remove impurities, and analyzed with 0.2M ammonia water. The analytical solution is concentrated and decolorized, and the total unit of the neomycin concentrate prepared is 190-200,000 units / ml , Adjust the PH value to 5-9, add neomycin sulfate concentrate to each column with a mass-volume ratio of 10%, and pump to the top of the weakly acidic cationic resin treatment column, open the liquid outlet valve, and control the flow rate to about 50ml / min, after the neomycin concentrate is adsorbed by weakly acidic cations, it is eluted with 0.8M ammonia solution to remove impurities, and the flow rate is controlled at 1 / 500-1 / 300 resin volume per minute. After the impurities are eluted, continue to elute neomycin C sulfate with 0.5M aqueous ammonia solution, and monitor the optical rotation of the solution at any time. When the optical rotation reaches the highest point and begins to ...

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Abstract

The invention relates to a preparation method of a medicine raw material, in particular to a method for extracting sulfuric acid fra avermectin from fermentation broth of neomycin sulfate. The preparation method is as follows: fermentation broth of neomycin sulfate is adsorbed by strong acidic ion exchange resin, parsed, concentrated and decolorized; the concentrated and decolorized concentrated solution of the neomycin sulfate is adsorbed by weak acidic cation exchange resin; ammonia of 0.1 M to 1.0 M is used to elute the neomycin sulfate B in the saturated resin in the first procedure; and eluent is concentrated, acidified, decolorized, spray dried and micronized, and sulfuric acid fra avermectin is prepared. The preparation method solves the problems existing in the prior art that the analytical yield is low, the raw materials are consumed a lot, the cost is high as the methanol is used in the crystallization process, the methanol pollutes the environment, and a lot of organic solvent remains in the product. The preparation method has the advantages that the adsorption selectivity of the resin is improved, the used amount of the eluent is reduced, no organic solvent remains in the product, the environmental pollution is reduced, the yield is improved, the quality of the product is improved, and the production cost is low.

Description

Technical field [0001] The invention relates to a method for preparing pharmaceutical raw materials, in particular to a method for extracting flamectin sulfate from neomycin sulfate fermentation broth. Background technique [0002] Flammectin sulfate (Neomycin B sulfate) is an aminoglycoside antibiotic isolated from neomycin sulfate. It has a good antibacterial effect on Staphylococcus aureus and Corynebacterium, and it has good antibacterial effect on Escherichia coli, Enterobacteria such as Klebsiella and Proteus also have a good effect, overcoming the side effects of neomycin sulfate, and can be made into injections, powders, and ointments. Freamectin sulfate is usually prepared by separating it from the fermentation broth of neomycin sulfate. The fermentation broth of neomycin sulfate is composed of neomycin B sulfate and neomycin C sulfate and some impurities. Among them, neomycin B sulfate and neomycin C sulfate are stereoisomers of each other, and neomycin B sulfate The co...

Claims

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Application Information

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IPC IPC(8): C07H5/06A61P31/04C12P19/28
Inventor 李瑞民陈学军赵亮闫贵霞杨彦芬
Owner SHIJIAZHUANG HUASHU PHARMA
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