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Method for extracting neomycin sulfate from neomycin sulfate fermentation broth

A technology of neomycin sulfate and fermentation broth, applied in chemical instruments and methods, preparation of sugar derivatives, sugar derivatives, etc. The problem of high content of mycin C, to achieve the effect of improving health level, high degree of automation, and reducing costs

Active Publication Date: 2015-05-13
河北圣雪大成唐山制药有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are many deficiencies in the above method, for example, the adsorption capacity of the static adsorption resin is low, the cost is high, the rinsing resin produces a lot of waste water, the ammoniacal solution makes the ammonia nitrogen content in the waste water high, the neomycin sulfate finished product yield is low, and the neomycin sulfate high in vitamin C

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] The neomycin sulfate fermentation broth was 50L, the titer was 25000U / ml, and the product weight was 1.25kg. The pH was adjusted to 2.0 with oxalic acid and filtered through a ceramic membrane. The pH of the filtrate was adjusted to 6.0 with an aqueous sodium hydroxide solution, and then pumped into the packed resin column of D152 resin with a peristaltic pump at a flow rate of 0.5 BV / h and an adsorption capacity of 200 g / L resin. Use 0.05mol / L sodium hydroxide aqueous solution to carry out the first elution to remove impurities, the flow rate is 0.1BV / h, and the volume is 3 times the resin volume. After the impurity elution is completed, use 0.2mol / L sodium hydroxide aqueous solution to carry out the second elution, the flow rate is 0.1BV / h to the pH of the effluent to 11.0, and top-wash with anhydrous salt until there is no titer. The eluate is subjected to ultrafiltration through an ultrafiltration membrane with a molecular weight of 3000, nanofiltration through a n...

Embodiment 2

[0039] The neomycin sulfate fermentation broth was 46L, the titer was 24300U / ml, and the product weight was 1.12kg. The pH was adjusted to 4.0 with oxalic acid and filtered through a ceramic membrane. The pH of the filtrate was adjusted to 8.0 with an aqueous sodium hydroxide solution, and then pumped into the packed resin column of D151 resin with a peristaltic pump, the flow rate was 2.0 BV / h, and the adsorption capacity was 180 g / L resin. Use 0.1mol / L sodium hydroxide aqueous solution to carry out the first elution to remove impurities, the flow rate is 0.5BV / h, and the volume is 5 times the resin volume. After the impurity elution is completed, use 2.0mol / L sodium hydroxide aqueous solution to carry out the second elution, the flow rate is 1.0BV / h to the pH of the effluent to 12.0, and top-wash with anhydrous salt until there is no titer. The eluate is subjected to ultrafiltration through an ultrafiltration membrane with a molecular weight of 10,000, nanofiltration throug...

Embodiment 3

[0041]Neomycin sulfate fermentation broth 58L, titer 23700U / ml, product weight 1.37kg. The pH was adjusted to 3.0 with oxalic acid and filtered through a ceramic membrane. The filtrate was adjusted to pH 7.0 with aqueous sodium hydroxide solution, and then pumped into the packed resin column of D152 resin with a peristaltic pump, the flow rate was 1.5 BV / h, and the adsorption capacity was 230 g / L resin. Use 0.07mol / L sodium hydroxide aqueous solution to carry out the first elution to remove impurities, the flow rate is 0.3BV / h, and the volume is 5 times the resin volume. After the impurity elution is completed, use 1.0mol / L sodium hydroxide aqueous solution to carry out the second elution, the flow rate is 0.5BV / h to the pH of the effluent to 11.5, and top-wash with anhydrous salt until there is no titer. The eluate is subjected to ultrafiltration through an ultrafiltration membrane with a molecular weight of 5000, nanofiltration through a nanofiltration membrane, salt transf...

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Abstract

The invention discloses a method for extracting neomycin sulfate from neomycin sulfate fermentation broth, and relates to a preparation method of a medicine raw material. The extraction method comprises filtering, resin absorption, prewashing, desorption, decolorization, desalination, salt conversion and spraying drying to obtain a finished product. The method has the following benefits: the problems of low static adsorption quantity, high cost, water resource waste, environmental pollution, low yield of finished neomycin sulfate products and high neomycin sulfate C content in the prior art are solved, and the method has the advantages of high production efficiency, low cost, little environmental pollution and good quality of the finished products.

Description

technical field [0001] The invention relates to a preparation method of pharmaceutical raw materials, in particular to a method for extracting neomycin sulfate from neomycin sulfate fermentation broth. Background technique [0002] Neomycin sulfate is an aminoglycoside antibiotic. This product has good antibacterial effect on Staphylococcus (methicillin sensitive strain), Corynebacterium, Escherichia coli, Klebsiella, Proteus and other Enterobacteriaceae bacteria. Cocci, Enterococcus and other poor activity. Pseudomonas aeruginosa and anaerobic bacteria are resistant to this product. Bacteria have partial or complete cross-resistance to streptomycin, neomycin, kanamycin and gentamicin. The current extraction method is that the fermentation broth of neomycin sulfate and the resin are mixed and stirred for static adsorption, the resin is rinsed, the ammonia solution is decomposed, the decolorized solution is decolorized with the resin, and then the film is concentrated, dec...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H15/232C07H1/06
CPCC07H1/06C07H15/232
Inventor 王婷谷新华
Owner 河北圣雪大成唐山制药有限责任公司
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