Detection method for avian influenza hemagglutination inhibition test

Abstract

The invention discloses a detection method for an avian influenza hemagglutination inhibition test. The detection method comprises the following steps: S1, sequentially numbering a V-shaped micro-reaction plate from 1 to 12, sequentially adding 50 [mu]L of 4 units of an avian influenza virus antigen solution into each of the holes from 1 to 11; S2, respectively adding 50 [mu]L of detected serum into the first hole and the twelfth hole, mixing evenly, then absorbing 50 [mu]L to the second hole from the first hole, sequentially diluting to the tenth hole at multiple proportions, abandoning 50 [mu]L in the tenth hole; S3, sequentially adding 50 [mu]L of 1% chicken erythrocyte suspension into each hole from the twelfth hole to the first hole; S4, oscillating the V-shaped micro-reaction plate on a microoscillator, allowing standing still, incubating, and judging the agglutination condition of each hole when erythrocyte in the twelfth hole forms a button shape and sinks to the bottom of thehole. The detection method is efficient and fast, is high in accuracy, and is suitable for monitoring large chicken flocks by an enterprise.

Description

technical field [0001] The invention relates to the field of microorganism detection, in particular to a detection method for hemagglutination inhibition test of avian influenza. Background technique [0002] Bird flu is an acute infectious disease caused by bird flu virus, which can infect humans. The main symptoms after infection are high fever, cough, runny nose, myalgia, etc. Most of them are accompanied by severe pneumonia. Multiple organ failures lead to death, and the case fatality rate is very high. Avian influenza viruses belong to the genus Influenza A in the Orthomyxoviridae family. Hemagglutination (direct hemagglutination) reaction is the use of virus or virus hemagglutinin to selectively agglutinate animal red blood cells and the virus concentration is positively correlated with the degree of red blood cell agglutination. Sensitive animal red blood cells are collected to prepare 1% red blood cell suspension and to be tested Serum and virus liquid are subjecte...

AI Technical Summary

Problems solved by technology

Since the avian influenza hemagglutination inhibition test needs to collect fresh blood, the amount of test materials and reagents involved in it is very small. Accurate determination is the key to the success of the test under the premise of a small amount of reagents. The national standard detection method requires physiological The serum to be tested is diluted with saline, and multiple incubations are required during the process. The steps are cumbersome and time-consuming. Multiple steps are linked together, resulting in a large increase in error and increasing the risk of accurate determination.

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