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Method for detecting regional SIV (swine influenza virus) subtype distribution

A swine flu virus, regional technology, applied in the field of animal health testing, can solve the problems of non-specific coagulation factor interference and difficult data analysis, and achieve the effect of eliminating interference and obvious effect.

Active Publication Date: 2017-02-22
SOUTH CHINA AGRI UNIV
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Problems solved by technology

[0005] In order to solve the problem that the hemagglutination and hemagglutination inhibition tests are interfered by non-specific coagulation factors and the data analysis is difficult, the present invention intends to provide a method for detecting the distribution of regional swine influenza virus subtypes, which is simple, effective, low-cost, and suitable for The development of local inspection work

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  • Method for detecting regional SIV (swine influenza virus) subtype distribution
  • Method for detecting regional SIV (swine influenza virus) subtype distribution
  • Method for detecting regional SIV (swine influenza virus) subtype distribution

Examples

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Embodiment 1

[0035] Example 1 Serological survey of influenza viruses in Jiangxi Province using a method for detecting the distribution of regional swine influenza virus subtypes

[0036] Through the serological monitoring and statistical analysis of H1 and H3 subtype swine influenza viruses in pigs in Jiangxi Province, the prevalence of influenza viruses in pigs in Jiangxi Province can be grasped.

[0037] Step 1. Collection of samples and information

[0038] From October 2012 to March 2015, a total of 2005 sera were collected covering the whole province of Jiangxi Province, including 130 samples in 2012 and 430 samples in 2013. In 2014 it was 924 and in 2015 it was 548. The serums of each city divided into districts are different, among which Fuzhou and Ji’an are the most, with 562 and 338, followed by Ganzhou, Shangrao, Nanchang, Jingdezhen, Yichun and Jiujiang, respectively 205, 172, 158, 155, 139 and 126 copies, of which Pingxiang City, Xinyu City and Yingtan City were the least, w...

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Abstract

The invention relates to a method for detecting regional SIV (swine influenza virus) subtype distribution and belongs to the technical field of animal hygiene examination. The method is characterized by comprising the following steps: acquiring serum samples firstly, processing the serum samples with a pancreatin-sodium ferrate-hydrogen peroxide-polyethylene glycol method, and removing non-specific clotting factors from the serum samples to obtain to-be-detected serum; determining the concentration of four hemagglutination units of antigens with a double-dilution method; reacting the to-be-detected serum, the to-be-detected antigens and chicken erythrocyte suspension on a V-shaped microtiter plate, and determining the to-be-detected serum with positive titer of serum antibodies by taking the highest dilution ratio, completely inhibiting the four hemagglutination units of the to-be-detected antigens, of the to-be-detected serum as the titer of the serum antibodies. The method is simple, effective and low in cost, can be used for performing positive rate analysis of a larger number of serum samples and lays a foundation for treatment and prevention of animal diseases.

Description

technical field [0001] The invention belongs to the technical field of animal health detection, and in particular relates to a method for detecting regional swine influenza virus subtype distribution. Background technique [0002] Swine Influenza Virus (SIV) belongs to Orthomyxoviridae and Influenzavirus A in classification. It is a single-stranded negative-strand RNA virus. The genome is composed of 8 independent segments of different sizes. Animal diseases, H1N1, H3N2 and H1N2 subtype swine influenza viruses are strains that are widespread in pigs and frequently cause disease in pigs. Simple SIV infection has the characteristics of high morbidity and low mortality, but swine influenza is a typical immunosuppressive disease, which will cause mixed or secondary infection of other pathogens, and cause serious economic losses. [0003] After the Spanish flu pandemic in 1918, the influenza virus infected swine herds evolved into the classical swine influenza (H1 / CS) branch. In...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569
CPCG01N33/56983
Inventor 张桂红甘平曹振鹏秦锋马骏卜德新
Owner SOUTH CHINA AGRI UNIV
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