Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Sperm antibody testing reagent

A technology of anti-sperm antibody and detection reagent, applied in the field of anti-sperm antibody detection reagent, can solve the problems of immune infertility diagnosis, treatment deviation, background noise, anti-sperm false positive, etc., and achieve easy results, simple detection and specificity strong effect

Inactive Publication Date: 2003-07-30
ANHUI ANKE BIOTECHNOLOGY (GRP) CO LTD
View PDF0 Cites 7 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, whether it is imported kits or domestic kits used in China, most of them are ELISA reagents. ELISA detection of anti-sperm antibodies mainly has the following shortcomings: 1. Due to the existence of soluble FC receptors in seminal plasma on the surface of sperm, the test results have serious background noise
2. The detected antisperm antibodies are free antibodies in body fluids, and only the binding antibodies on the surface of sperm are the main factors affecting fertilization, so the results of ELISA lead to clinical deviations in the diagnosis and treatment of immune infertility
3. Because many proteins on the surface and inside of sperm are the same or similar to the proteins on the body tissue cells, resulting in false positives in ELISA detection of anti-sperm

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0035] The anti-sperm antibody detection reagent is composed of the following components in weight ratio:

[0036] 2.5% sensitized sheep red blood cell suspension 20ul

[0037] 1:2 9 Rabbit anti-human IgG 20ul

[0038] The method of making the above-mentioned anti-sperm antibody detection reagent:

[0039] A. Production process of 2.5% sensitized sheep red blood cell suspension

[0040] 1. Choose 1 healthy young sheep, take 50ml blood without jugular vein, store the blood in Alsever solution at 4℃, and shake it well.

[0041] 2. Take 50ml of the above fresh sheep red blood cell solution and wash it 3 times in a centrifuge with physiological saline, and the process condition is 500 rpm.

[0042] Three, hydroformylation treatment

[0043] Take PH7.2 phosphate buffer to make a 5% suspension, slowly drop 2.5% glutaraldehyde on a shaker, and continue to shake for 60 minutes after the addition;

[0044] Wash the above sheep red blood cell suspension 5 times with physiological saline in ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The sperm antibody testing reagent includes sensitized sheep erythrocyte suspension of 2-3 % concentration and rabbit antihuamn IgG in the concentration of 1 to 2 to the (8-10)-th power. The sensitized sheep erythrocyte suspension of 2-3% concentration is prepared through taking sheep blood, washing, hydroformylation, tanning, sensitizing and other steps. The preparation of rabbit antihuman IgG in the concentration of 1 to 2 to the (8-10)-th power includes taking rabbit blood, separating blood serum and other steps. The reagent has strong specificity, high detection sensitivity, no false positive and no false negative phenomenon, high stability, and storage life as long as one year. Using the reagent can obtain easy-to-judge detection result sipmle and fast.

Description

Technical field [0001] The invention relates to an anti-sperm antibody detection reagent. Background technique [0002] Immune factors are one of the causes of male infertility. According to the classification of the causes of human infertility by WHO, the immune factors in the causes of infertility are defined as: normal sex and ejaculatory function, in at least one semen specimen, mixed antiglobulin reaction test (MAR) or Immune Bead Test (IBT) No less than 50% of the motile sperm surface is coated with antibodies. In 2000, it was clearly stipulated that only this test can be used as the diagnostic criteria for immune infertility in the diagnosis of infertility. [0003] The domestic anti-sperm antibody detection mainly uses the classic agglutination test, immobilization test and enzyme immunoassay (ELISA). The latter is coated with intact sperm or purified sperm antigen after death, and the enzyme-labeled anti-human antibody is used with the corresponding substrate.物色。 The obj...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N33/50G01N33/563
Inventor 徐振山杨玲杜贤宇李平生宋礼华
Owner ANHUI ANKE BIOTECHNOLOGY (GRP) CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com