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265results about How to "The result is easy to judge" patented technology

Test paper strip for detecting Porcine Parvovirus and porcine encephalitis B virus antibody

The invention discloses a test strip that is used for detecting antibodies of porcine parvovirus and porcine encephalitis B virus in one step, which comprises a supporting layer, an adsorptive layer and a protecting layer, and the adsorptive layer consists of a sample fiber layer, an adsorptive gold mark fiber layer, a fibrin membranous layer and a water absorptive material layer sequentially from a testing end; the adsorptive gold mark fiber layer adsorbs any one or two from antigen liquids of gold colloid marked SPA or gold colloid marked pure PPV and JEV; a detecting print and a contrast print are arranged on the fibrin membranous layer, the detecting print is printed with any one or two from the purified PPV and JEV antigen liquids, and the contrast print is printed with an IgG solution of ovine or rabbit anti-SPA or an IgG solution of any one or two viruses from ovine or rabbit anti-PPV and anti-JEV. The test strip of the invention has strong detecting specificity and high sensitivity, avoids additional equipment and reagents, can be operated by every person, and is particularly applicable to the quick specific antibody detection of porcine diseases on the spot.
Owner:HENAN ACAD OF AGRI SCI

Test reagent for realizing online rapid nondestructive test of quality of gold plating layer and using method of test reagent

The invention discloses a test reagent for realizing the online rapid nondestructive test of the quality of a gold plating layer and a using method of the test reagent. The reagent contains trifluoroacetic acid and copper sulfate pentahydrate. The using method comprises the following steps: dropwise adding 2-3 drops of test reagent in a to-be-detected region of the gold plating layer, wiping the gold plating layer by using dust-free cloth after 60-120s, and observing the color change condition of the gold plating layer; if the color of the gold plating layer is changed, judging that a workpiece is a defective product; if the color of the gold plating layer is unchanged, judging that the plating layer is qualified. The test reagent disclosed by the invention is rapid in reaction, an obvious color difference exists between a generated corrosion product and the plating layer, and a result is high in accuracy and easy to judge; the test reagent is extremely simple in operation, no special instruments are needed, and online detection can be realized; the test reagent can be used for realizing nondestructive test, and workpieces which are qualified through test can be continued to be processed, assembled and circulated on a production line, so that the resource is saved; the test reagent is low in cost but capable of accurately reminding a manufacturer of stopping the subsequent processing of a defective product of the plating layer and taking remediation measures in time, so that more loss is avoided.
Owner:TANTZ ENVIRONMENTAL TECH

Method for quickly and nondestructively distinguishing between real and fake bird's nests

The invention discloses a method for quickly and nondestructively distinguishing between real and fake bird's nests. The method is characterized in that Raman spectrometry is adopted; pretreatment is not required to be performed on samples; technical parameters are directly set; Raman spectra is collected through a Raman spectrometer; whether characteristic peaks of bird's nests appear or not is found and the quantity is determined. If 3 to 6 characteristic peaks of bird's nests appear and other obvious impure peaks do not exist, the samples are manifested to be real bird's nests; if 3 to 6 characteristic peaks of bird's nests appear and other obvious impure peaks exist, the samples are manifested to be fake bird's nests; the adulterate amount is roughly judged according to peak height ratio of the impure peaks and the characteristic peaks of the bird's nests, the higher the ratio is, the lower the bird's nest content is; if 0 to 2 characteristic peaks of the bird's nests appear, the samples do not contain bird's nest components. The method has the characteristics that the samples are not required to be destroyed, the method is simple to operate, discrimination speed is high, the accuracy is high, and the like, is applied to the discrimination of large-scale bird's nest samples, and is novel.
Owner:徐敦明

Bacillus coli O157:H7 antigen rapid detection testing strip

The test strip for fast detecting colibacillus O157:H7 antigen belongs to the field of medical diagnosis article technology. The fast test strip includes one base substrate, one fiber film covering the base substrate, one water absorbing film covering the upper part of the base substrate, monoclonal marking antibody of colibacillus O157:H7 antigen adsorbed onto the fiber film in the lower part of the test strip, one anti-mouse IgG coated quality controlling line on the middle part of the fiber film, and one detecting line coated with monoclonal antibody of colibacillus O157:H7 antigen with excellent pairing property to absorbed marking antibody below the quality controlling line. The marking antibody has marker of colloidal gold, the concentration of colloidal gold antibody is (1.0-3.0)x10<->3 mg / sq cm, and that of the coating antibody is (2.0-8.0)x10<->4 mg / sq cm. The present invention has high detection speed and high detection accuracy.
Owner:贝瑞特生物技术(郑州)有限责任公司

Rapid detection card for detecting pesticide residues, preparation method and application thereof

The invention relates to a rapid detection card for detecting pesticide residues, a preparation method and application thereof. The fast detection card comprises a coverage film, a first plastic board, sample loading holes, an enzyme tablet, a plastic film, a second plastic board and a substrate sheet, wherein the coverage film is adhered to the surface of the first plastic board; at least two sample loading holes are formed in the first plastic board; the enzyme tablet fixed with housefly acetylcholinesterase is adhered to the reverse sides of the sample loading holes and is opposite to the sample loading holes; the substrate sheet, which is fixed with acetyl indophenol and is adhered to the position corresponding to the enzyme tablet, is arranged on the second plastic board; and the plastic film is arranged in the middles of the first plastic board and the second plastic board. In the rapid detection card, enzyme is the housefly acetylcholinesterase which is subjected to molecular evolution, so the rapid detection card has the advantages of high activity, high sensitivity, high stability and the like and can be applied to the rapid detection of organophosphorus and carbamic acid ester pesticide residues in agricultural products, food and soil and particularly vegetables, fruits.
Owner:SHANGHAI ACAD OF AGRI SCI

Eye fundus image judgment method and equipment

The invention provides an eye fundus image judgment method and eye fundus image judgment equipment. The eye fundus image judgment method comprises the following steps: acquiring a to-be-judged eye fundus image of a user; Obtaining a reference fundus image associated with the fundus image to be judged, wherein the reference fundus image is a fundus image with a known state being normal; Generatinga difference image according to the to-be-judged fundus image and the reference fundus image; And taking the differential image as input data of a machine learning model, enabling the machine learningmodel to output a judgment result of the to-be-judged fundus image, and training the machine learning model by using the training differential image and corresponding label data.
Owner:SHANGHAI EAGLEVISION MEDICAL TECH CO LTD

LAMP primer of staphylococcus aureus and application method of LAMP primer

The invention discloses an LAMP primer of staphylococcus aureus. The LAMP primer comprises two inner primers (FIP and BIP) and two outer primers (F3 and B3), namely, F3 as shown in the sequence SEQ ID NO:1, B3 as shown in the sequence SEQ ID NO:2, FIP as shown in the sequence SEQ ID NO:3 and BIP as shown in the sequence SEQ ID NO:4. Aiming at the specific nuc gene of the staphylococcus aureus, a group of primers is designed, and a sensitive and specific staphylococcus aureus LAMP detection method is established by using the primers. The experiment shows that the detection sensitivity and the detection limit of the LAMP detection method are 100 times of those of a PCR method; whether green fluorescence is generated or not can be observed with naked eyes so as to confirm whether the reaction is performed, so that the result judgment can be simple, convenient and feasible; compared with a PCR technique, the method disclosed by the invention has the advantages that the time and the labor can be saved, no expensive instruments or tedious electrophoretic analysis is needed, and the application method disclosed by the invention is particularly applicable to use in base levels.
Owner:河北省食品检验研究院

Toxoplasmosis IgM antigen testing reagent and its application

The invention discloses an IgM antibody detection agent for toxoplasma, wherein it uses anti-human IgM-mu chain mono-clone antibody to pack the micro-pore plate, marking with the toxoplasma antigen and enzyme-label antigen for toxoplasma antigen and other assist agents to prepare the detection agent. This invention has high sensitivity and well specificity fit to convenient operation.
Owner:ZHUHAI S E Z HAITAI BIOLOGICAL PHARMA

Olive oil identification method and ion mobility spectrometry spectrum of olive oil standard substance

The invention discloses an olive oil identification method and an ion mobility spectrometry spectrum of an olive oil standard substance. The identification method comprises the following steps: weighing 50 microliters of a sample and taking the sample into a 50mL test tube, adding 5 mL of a n-hexane solution, continuously adding 2mL of a methanol solution of 1 mol/L potassium hydroxide, and carrying out vortex mixing; heating in 40 DEG C water-bath for 30 min and cooling to room temperature; adding 1 mL of deionized water into the test tube, and carrying out vortex mixing; centrifuging at 5000 r/min for 3 min, taking a supernatant into a 10mL test tube, and drying by nitrogen-blow; carrying out redissolution with 2 mL of methanol, sieving with a 0.22micron filter membrane and waiting to be identified by a machine; setting technical parameters, and acquiring a spectrum of the sample through ion mobility spectrometry; and whether the sample is pure olive oil is judged by comparison between ion mobility spectrometry spectra of olive oil and the sample to be tested. The method has characteristics of simple operation, rapid identification, high accuracy and the like, is suitable for determination of large-batch samples, and is a new method for rapidly identifying the authenticity of olive oil.
Owner:徐敦明
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