The present invention discloses a coloring culture medium for detecting food-borne pathogenic Yersinia, wherein the culture medium contains soybean peptone, yeast powder, sodium chloride, sodium oxalate, agar, a beta-riboside chromogenic substrate, a beta-galactoside chromogenic substrate, esculin, ammonium ferric citrate, bile salt No.3, cephalosporin, novobiocin, and the balance of water. According to the present invention, the coloring culture medium has characteristics of simple formula and convenient preparation operation, can be used for the separation and the detection of food-borne pathogenic Yersinia, and further has advantages of specificity, high efficiency, rapidness, low cost, simple result determination, wide application prospect, and the like.