Corynebacterium glutamicum and application thereof
A Corynebacterium glutamicum and glutamine technology, applied in the field of microorganisms, can solve the problems of low L-glutamine production and cannot meet the large-scale industrial production of L-glutamine, and achieve increased yield and improved synthesis effect of ability
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Embodiment 1
[0041] Embodiment 1: the screening of Corynebacterium glutamicum MHZ-0510, MHZ-0511 of the present invention
[0042] The starting strain - wild type Corynebacterium glutamicum B498 was inoculated in LB liquid medium and cultivated at 30°C for 10 hours to obtain a bacterial suspension in the logarithmic growth phase, and then spread the bacterial suspension on the LB solid plate. Appropriate amount of N-methyl-N'nitro-N-nitrosoguanidine solid particles were placed in the center of the LB solid plate, placed in an incubator at 30°C for 16 hours and then scraped to remove N-methyl-N'nitro- N-nitrosoguanidine particles are surrounded by a weak lawn of bacteria suspended in an appropriate amount of sterile saline. Spread the normal saline with bacterial lawn suspended on the LB solid plate containing 1.0 mg / L streptomycin or neomycin respectively, continue to culture at 30°C for 2 days, and pick a single colony according to the characteristics of the colony shape, color, etc., amo...
Embodiment 2
[0046] Embodiment 2: L-glutamine production method of the present invention and L-glutamine fermentation comparative test
[0047] 1. Fermentation method
[0048] Corynebacterium glutamicum MHZ-0510 and MHZ-0511 described in the present invention were respectively inoculated in the seed medium described below for propagation, cultured with shaking at 30°C for 16 hours, and the resulting culture was inoculated into the fermentation described below In the culture medium, the volume ratio of the culture medium to the fermentation medium is 1:10 (those skilled in the art can adjust it according to the amount of the fermentation medium in actual production, this ratio is only the preferred ratio), shake culture at 30°C until no longer Until L-glutamine is produced (generally about 72 hours). During shaking culture in the fermentation medium, maintain the pH value in the fermentation medium between 6.5-7.4.
[0049] Seed medium: 20g / L glucose, 0.5g / L potassium dihydrogen phosphate...
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