86results about How to "Reduce non-specific reactions" patented technology

The invention relates to a novel coronavirus advantage epitope fusion protein, a diagnosis reagent and an application, and belongs to a viral diagnosis reagent. The novel coronavirus advantage epitopefusion protein comprises an N protein advantage epitope peptide and an S protein advantage epitope peptide which are connected through a connecting arm, and an E protein advantage epitope peptide, wherein the N protein advantage epitope peptide comprises one or two or above of an advantage epitope peptide N1, an advantage epitope peptide N2, an advantage epitope peptide N3 and an advantage epitope peptide N4. The novel coronavirus advantage epitope fusion protein is high in specificity, high in sensitivity (higher in positive detection rate), high in biocompatibility, high in solubility and good in stability, and is favorable for preparation of a corresponding detection reagent. The detection reagent prepared from the fusion protein is high in sensitivity and good in specificity, and early screening of novel coronaviruses can be realized.

The invention relates to a dilute of an alkaline phosphatase maker and application thereof. The diluent comprises a buffer, a protein, a surfactant, a zinc ion, a magnesium ion, a preservative and anadditive, wherein the additive comprises a blocking agent and / or alkaline phosphatase; and the alkaline phosphatase is a low-active alkaline phosphatase and / or inactive alkaline phosphatase. The invention creatively adds the blocking agent and / or alkaline phosphatase to the traditional diluent for diluting alkaline phosphatase markers, and can well reduce non-specific reactions and ultimately reduce the probability of false positive results in clinical samples to improve the accuracy of detection and analysis results.

The invention relates to a preparation method of a new human TDP-43 monoclonal antibody coating ELISA plate and ELISA detection kit. The key technology mainly lies in that: genetic engineering method is utilized to prepare a specific mouse anti-human monoclonal antibody, and the ELISA plate by which the antibody is purified and then coated is taken as important component of the kit, and then the ELISA plate, TDP-43 polyclonal antibody and horseradish peroxidase labelled second antibody, TDP-43 standard substance, TDP-43 positive control, sample diluent, cleaning solution, development solution and stop solution are assembled into a humanized TDP-43 ELISA detection kit; a sensitive and rapid method for detecting concentration of TDP-43 in ELISA antibody capturing human serum or cell and tissue culture is established, and the method has high specificity and high stability. The kit is mainly used in general libratory or neuropathology clinical research.

The invention discloses a magnetic particle chemiluminiscence detection kit for heparin-binding protein, a preparation method of the magnetic particle chemiluminiscence detection kit and application of the magnetic particle chemiluminiscence detection kit to immunological detection of the heparin-binding protein. The magnetic particle chemiluminiscence detection kit comprises a streptavidin-coatedmagnetic particle suspension solution, a biotin-labeled heparin-binding protein capture antibody and biotin-labeled bovine serum albumin mixed solution, a luminous marker coupled heparin-binding protein detection antibody solution, a heparin-binding protein series calibration product and a quality control product. The kit provided by the invention utilizes the advantages of a magnetic particle chemiluminescence detection method, and has the characteristics of simple operation, strong specificity, high sensitivity, wide linear range and the like when a full-automatic chemiluminescence immunoassay analyzer is used for detecting the heparin-binding protein in a specimen.

The invention discloses a fluorescent chemosensor for detecting a transcription factor NF-kappaBp50 and a detection method thereof. The fluorescent chemosensor takes 2-aminopurine (2-AP) as a fluorescent group, target-driven spontaneous isothermal amplification and exonuclease-assisted fluorescent signal amplification strategies are adopted, ultrasensitive detection of the transcription factor is realized under the condition that no extract primer and template participates in, operation is easy and rapid, and test results are accurate and reliable. Experiments verify that detection limit of the technical scheme reaches 4.04*10<-4>mg / ml and can be comparable with a real-time fluorescent quantitative PCR method. Therefore, the technical scheme of the invention has extremely high popularization and application values.

The invention discloses a kit for detecting non-mucus type klebsiella pneumoniae with high virulence, and belongs to the technical field of biological detection. An ampR gene sequence in a non-mucus type klebsiella pneumoniae genome with high virulence is used as a marker, primers are designed based on the specific sequence, and finally qualitative detection of the non-mucus type klebsiella pneumoniae with high virulence is completed based on a polymerase chain reaction to make up for the situation that a qualitative detection method of klebsiella pneumoniae in the prior art can determine thevirulence of the klebsiella pneumoniae only by judging whether a mucus type exists or not, but cannot distinguish the difference in virulence among non-mucus type strains, so that the clinical application value of the marks and kit for detecting the non-mucus type klebsiella pneumoniae with high virulence and an application method of the kit is ensured.

[PROBLEMS] To provide a soluble highly sensitive probe complex with high functional capability ensuring usability in immunoassays of high sensitivity. [MEANS FOR SOLVING PROBLEMS] A highly sensitive probe complex can be produced by linking hydrophilic intermediums to a carrier and linking detection markers such as biotin, haptens or low-molecular-weight peptides and antibodies to the intermediums. By virtue of the linking of hydrophilic intermediums to a carrier, a multiplicity of hapten molecules can be linked and the probe complex as a whole becomes hydrophilic, so that there can be obtained a probe complex with high functional capability which would not induce nonspecific reactions.

The invention discloses a tree-like marker including nucleic acid, fluorescence molecule and protein / compound; a method for preparing the tree-like molecular; and an immunity detection reagent kit for forming the tree-like marker. The fluorescence molecule and the nucleic acid used as vector are combined into a signal marker in non-embedded manner, the signal marker and the protein or compound are bonded in non-covalent bond to from a tree-like structure; the label method is used for all immunity tests (tumor marker, communicable disease, hormone, heart disease, food contaminant, environmental pollutant, etc.), can obviously increase signal molecular number in immunity detection, and greatly improve detection sensitivity.

The invention relates to an agent box and method for measuring the livestock bilharziasis antibody, which uses the soluble Japan schistosome ovum antigen as sample antigen, and uses the colloidal gold to label Staphylococcus aureus A protein. Said invention combines the diagnosis with the technique which uses colloidal gold to label protein and sets up the spot electrosyneresis method, and the agent box based on this technique has high sensitivity and the result can be discriminated with naked eye. The test has proved that the method measuring the Schistosoma bovis has the sensitivity of 93.3 %, specificity of 98.0%, the index is 0.913.

The invention discloses a method for detecting toxicity of non-mucus klebsiella pneumoniae, and belongs to the technical field of biological detection. The method comprises the following steps: takingan ampR gene sequence in a high-toxicity non-mucus klebsiella pneumoniae genome as a marker, designing a primer by using the specific sequence, and finally completing qualitative detection on the toxicity of the non-mucus klebsiella pneumoniae based on polymerase chain reaction. The method overcomes the defect that: the toxicity of the klebsiella pneumoniae can only be judged by judging whether the klebsiella pneumoniae is mucus or not and the toxicity difference in non-mucus strains cannot be distinguished in qualitative detection methods of the klebsiella pneumoniae in the prior art, so that the application values of the marker and the detection method for detecting the toxicity of the non-mucus klebsiella pneumoniae are guaranteed.

The invention discloses a single-chain antibody of an anti-schistosome monoclonal NP11-4 as well as a preparation method and application thereof, and relates to the field of gene engineering and the field of therapeutic drugs against schistosomiasis. The invention is to use a monoclonal antibody NP11-4 located at adult membrane, cercaria memberane, schistosomulum membrane, egg shell and miracidium in egg of Schistosoma japonicum, extract total RNA of hybridoma cells through a gene engineering technology, adopt RT-PCR for proliferation of genes VH and VL, and use overlap-extension PCR to connect the genes VH and VL into a single-chain antibody gene in the form of VH<-linker-VL, which is subsequently connected with a pBAD / gIIIA carrier and cloned into Top10F'of Escherichia Coli to induce a soluble expression of the target gene. The zone from amino acid No.1 to No. 122 of the expressed single-chain antibody of the anti-schistosome monoclonal NP11-4 is a heavy chain gene repertoire of the single-chain antibody (VH), and the zone from the amino acid No.141 to No.254 is a light chain gene repertoire (VL) of the single-chain antibody, wherein the linker between the heavy and light chain gene repertoires consists of 18 amino acids including repeated glycines and serines, and the single-chain antibody has 254 amino acids in full length.

An enzymonimmune reagent kit for testing the IgM antibodies of 4 prenatal pathogens (TOX, RV, CMV and HSV) for gravida is composed of box, perforated plate, oprating liquid including washing liquid, enclosing liquid, diluting liquid, enzyme substrate and enzyme-linked matter resisting human IgM, and the liquid sealed in the holes on plate, which contains dicarbonate, sodium carbonate, urea, guanidine hydrochloride and distilled water for test antigen of TORCH rivus and TORCH-IgM antibody. Its advantages are easy operation and high effect.