The present invention relates to a preparation method of a medical adhesive with high antibacterial performance, which is characterized in that it comprises the following steps: step 1, adding sodium alginate, lignin, tannic acid, and a stabilizer Bacterial water, heated to 100°C, stirred and dissolved for 150min, cooled to room temperature to obtain aqueous solution A; step 2, poly(α-isobutyl cyanoacrylate-α-octyl cyanoacrylate), polyurethane, polyisoprene According to the ratio of parts, add alkenes to seabuckthorn seed oil, heat to 80°C and stir for 75min, cool to room temperature to obtain seabuckthorn seed oil solution B; step 3, mix the above aqueous solution A and seabuckthorn seed oil solution B evenly, and Add high-efficiency antibacterial components and formaldehyde inhibitors, and stir for 4 hours; Step 4, filter and sterilize the product obtained in Step 3, subpackage, seal, and package to obtain a medical adhesive with high antibacterial performance.