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Root-knot nematode resistant pumpkin stock and breeding method and application thereof

A technology for resisting root-knot nematodes and root-knot nematodes is applied in the directions of application, horticultural methods, botanical equipment and methods, etc., and can solve the problems that cannot be used to control root-knot nematodes, and the resistance of resistant materials is low, so as to reduce chemical The effect of pesticide use, human and environmental safety, compatibility and compatibility assurance

Inactive Publication Date: 2019-08-23
INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, cucumber genetic breeding materials resistant to root-knot nematode have not been found in cucumbers, so screening root-knot nematode-resistant materials from other cucurbit crops and using them as rootstocks for cucumber grafting to prevent nematode damage has become an important research direction
[0003] Pumpkin is a commonly used rootstock for grafting with cucumber in production, such as "black-seeded pumpkin" and "white-seeded pumpkin", etc. have commercial products, but these pumpkin rootstocks are usually used to prevent and control soil-borne diseases such as cucumber wilt, although researchers have Some nematode-resistant rootstocks of Cucurbitaceae have been screened, but these resistant materials have low resistance and cannot be used in production to control root-knot nematodes

Method used

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  • Root-knot nematode resistant pumpkin stock and breeding method and application thereof
  • Root-knot nematode resistant pumpkin stock and breeding method and application thereof
  • Root-knot nematode resistant pumpkin stock and breeding method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] A high-throughput inoculation, identification and screening method for root-knot nematode-resistant NR pumpkins, specifically comprising the following steps:

[0028] (1) 71 parts of pumpkin farm varieties were collected from all over the country, and 10 seeds of each part were germinated and planted in a seedling pot of 10 × 10 cm, one plant per pot, cultivated with sterilized peat: vermiculite (2: 1) Substrate, each material was repeated three times, and normal seedling management was carried out;

[0029] (2) When the two cotyledons of the pumpkin seedlings were unfolded and the first true leaf appeared, high-throughput root-knot nematodes were inoculated in the seedling pot, and each plant was inoculated with 30,000 second-instar larvae of root-knot nematode incognita. After 30 days, gently wash off the substrate on the root system of the seedlings to identify the resistance of pumpkins to root-knot nematodes, and select pumpkin materials with high resistance;

[0...

Embodiment 2

[0043] A kind of breeding method of NR pumpkin stock and cucumber grafted seedling comprises the following steps:

[0044] (1) The nematode-resistant NR pumpkin and cucumber seeds (Zhongnong No. 6) were soaked in warm soup for seed disinfection treatment, and sterile water was used for germination. The cucumber seeds were sown 2 days before the pumpkin, and sowed in 10×10cm seedlings respectively In the pot, sterilized peat: vermiculite (2:1) was used as the culture substrate for normal seedling management.

[0045] (2) When the resistant pumpkin (NR pumpkin) was sown for 13 days, the cotyledons of pumpkin and cucumber seedlings flattened, and the first true leaf appeared. At this time, the stem thickness of pumpkin rootstock and cucumber scion was similar, which was the best time for grafting. When grafting, use a sterilized sharp blade to cut obliquely from top to bottom at 0.8 cm above the cotyledon node of the pumpkin stalk. The section is at an angle of 45 degrees to the ...

Embodiment 3

[0048] A kind of breeding method of NR pumpkin stock and cucumber grafted seedling comprises the following steps:

[0049](1) The nematode-resistant NR pumpkin and cucumber seeds (Zhongnong No. 6) were soaked in warm soup for seed disinfection treatment, and sterile water was used for germination. The cucumber seeds were sown 2 days before the pumpkin, and sowed in 10×10cm seedlings respectively In the pot, sterilized peat: vermiculite (2:1) was used as the culture substrate for normal seedling management.

[0050] (2) When the resistant pumpkin (NR pumpkin) was sown for 13 days, the cotyledons of pumpkin and cucumber seedlings flattened, and the first true leaf appeared. At this time, the stem thickness of pumpkin rootstock and cucumber scion was similar, which was the best time for grafting. When grafting, use a sterilized sharp blade to cut obliquely from top to bottom at 1 cm above the cotyledon node of the pumpkin stalk. The section is at an angle of 45 degrees to the ste...

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Abstract

The invention provides a root-knot nematode resistant pumpkin stock and a breeding method and an application thereof. The breeding method thereof adopts an inoculating screening method for largely collecting farm varieties and performing high-throughput, a highly-resistance pumpkin material is rapidly detected, at the same time, technologies of continuous selfing and single-pumpkin breed conservation are adopted in breeding, stable inheritance of resistance can be tested within a shorter time, and the method is high-efficient in screening. At the same time, the effect, to root-knot nematode, of cucumber seedlings grafted by the screened pumpkin stock can reach 73.5% in pot culture and field preventing experiments, and is better than control efficiency of a chemical pesticide, so high efficiency of preventing application is reflected.

Description

technical field [0001] The invention belongs to the technical field of stock breeding, and in particular relates to root-knot nematode-resistant pumpkin stock and its breeding method and application. Background technique [0002] Root-knot nematode is an important pathogen of cucumber, which causes serious losses to cucumber production every year, with a 20-30% yield reduction, and in severe cases, no harvest. It is an important reason for cucumber continuous cropping obstacles. However, no root-knot nematode-resistant cucumber genetic breeding materials have been found in cucumbers. Therefore, screening root-knot nematode-resistant materials from other cucurbit crops and using them as rootstocks for cucumber grafting to prevent nematode damage has become an important research direction. [0003] Pumpkin is a commonly used rootstock for grafting with cucumber in production, such as "black-seeded pumpkin" and "white-seeded pumpkin", etc. have commercial products, but these pu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G22/05A01G2/30A01H1/02A01G7/06
CPCA01G7/06A01H1/02A01G2/30A01G22/05
Inventor 茆振川谢丙炎凌键杨宇红殷楠
Owner INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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