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Chemical luminescence immune assay determination reagent kit for rubella virus IgG antibody and preparation method thereof

A chemiluminescence immunity and chemiluminescence technology, which is applied in the field of immunoanalysis medicine, can solve the problems of being unable to be widely used in clinical diagnosis and scientific research work, and restricting the use of popularization, so as to reduce non-specific reactions, improve specificity and sensitivity, and dilute The effect of scaling down

Inactive Publication Date: 2009-02-18
CHEMCLIN DIAGNOSTICS CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In addition, the chemiluminescence immunoassay kit in the prior art is a closed fully automatic chemiluminescence measurement system, which requires an expensive fully automatic chemiluminescence measuring instrument, thus limiting its popularization and use, and cannot be widely used in clinical diagnosis and scientific research.

Method used

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  • Chemical luminescence immune assay determination reagent kit for rubella virus IgG antibody and preparation method thereof
  • Chemical luminescence immune assay determination reagent kit for rubella virus IgG antibody and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1 Preparation of rubella virus IgG antibody chemiluminescent immunoassay assay kit of the present invention

[0024] The rubella virus IgG antibody diagnostic kit of the present invention comprises: negative and positive controls, coated rubella virus antigen solid-phase carrier, sample diluent, concentrated washing solution, alkaline phosphatase-labeled anti-human IgG monoclonal antibody, chemiluminescent substrate thing.

[0025] The inventors of the present invention firstly carried out the screening test and quality identification of the raw materials used, including the adsorption performance and variation size of the coated antigen, labeled antibody, white microwell plate, ALP activity, luminescence intensity and luminescence intensity of the chemiluminescence substrate. duration etc. Then test the solid phase carrier with different coating buffers and blocking solutions, select the most suitable coating buffer and blocking solution, and find the best con...

Embodiment 2～3

[0073] Embodiment 2~3 prepare rubella virus IgG antibody chemiluminescence immunoassay assay kit of the present invention

[0074] The assay kit of the present invention was prepared in the same manner as in Example 1 except that plastic beads and plastic tubes were used as carriers respectively.

Embodiment 4

[0075] Embodiment 4 The using method of kit of the present invention

[0076] 1) Take out the kit from the refrigerator at 4°C, and equilibrate at room temperature for 15 minutes.

[0077] 2) After diluting the 20-fold concentrated washing solution provided in the kit with deionized water 20 times, it is prepared as an application washing solution.

[0078] 3) Take out the coated slats and insert them into the plate holder.

[0079] 4) Set 3 wells for negative control and 2 wells for positive control, and add 100uL to each well. Add 100uL of the sample diluent to the well, then add 10uL of the serum to be tested into the sample diluent, and set a blank well for each experiment. Shake and mix well with a micro shaker, paste the sealing film, and incubate at 37°C for 30 minutes;

[0080] 5) Wash the plate 5 times with a fully automatic plate washer or manually, 400uL per well, and finally dry it on clean absorbent paper;

[0081] 6) Except for blank wells, add 100uL of enzym...

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Abstract

Disclosed is a chemiluminescence immunoassay test kit which is used to detect rubella IgG antibody, the test kit includes: positive and negative reference substance, solid-phase carrier which is coated by rubella virus antigen, sample diluent, antihuman IgG monoclonal antibody labeled by alkaline phosphatase, chemiluminescence zymolyte and condensed washing liquid. The preparing method of the test kit includes steps as following: 1) the positive and negative reference substance is prepared; 2) the solid-phase carrier is coated; 3) the sample diluent is prepared; 4) the antihuman IgG monoclonal antibody is labeled by the alkaline phosphatase; 5) the chemiluminescence zymolyte is prepared; 6) the condensed washing liquid is prepared; 7) the positive and negative reference substance, the sample diluent, the alkaline phosphatase labeled compound, the chemiluminescence zymolyte and the condensed washing liquid can be filled and packed through separate packing; 8) finished product can be made after assembly. Adopting the test kit and the preparing method, the rubella IgG antibody in the human blood serum can be detected simply, quickly, sensitively and accurately, thereby being convenient for popularization and application.

Description

technical field [0001] The invention relates to the field of immunoanalysis medicine, in particular, the invention provides a rubella virus IgG antibody chemiluminescent immunoassay assay kit and a preparation method thereof. Background technique [0002] Rubella virus belongs to the Togaviridae family, with single-stranded positive-strand RNA, 60nm in diameter, and only one serotype. The antigenic structure of rubella virus is quite stable, and it is one of the most dangerous teratogenic factors. Rubella virus is named TORCH together with cytomegalovirus, toxoplasma virus, and herpesvirus types 1 and 2. The common harm of these five major viruses is to cause miscarriage, premature birth, stillbirth, fetal malformation, and disabled children. Newborns who survive by chance often suffer from growth retardation, cataract, nerve deafness, congenital heart disease, meningoencephalitis, giant cell hepatitis, hemolytic anemia, visual and hearing impairment, etc. Therefore, TORCH...

Claims

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Application Information

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IPC IPC(8): G01N33/577G01N33/569G01N21/76
Inventor 赵娟娟应希堂宋胜利胡国茂唐宝军张坤
Owner CHEMCLIN DIAGNOSTICS CO LTD
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