Enzymoimmune reagent kit and its production method
A kit and enzyme immunology technology, which is applied in biological testing, material inspection products, measuring devices, etc., can solve problems such as interference and high detection costs, and achieve the effects of simplified operation, high sensitivity, and stable colored products
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[0051] The invention comprises a box body, a porous strip arranged in the box body and a liquid used in the box body, and each hole of the porous strip is coated with the TORCH antigen that can cause fetal congenital malformation to detect TORCH in blood of pregnant women -IgM antibody, prepare and mix the coating solution and other solutions for later use, first use the coating solution (0.05mol / L, pH9.6 carbonic acid buffer plus 0.003mol / L urea and 0.003mol / L guanidine hydrochloride) Dilute the TORCH antigen so that the final concentrations are: TOX antigen 10 μg / 100ml, RV antigen 170 μg / 100ml, CMV antigen 110 μg / 100ml, HSV-1 antigen 110 μg / 100ml. Place the TORCH antigen suspension at 37°C for 2 hours, then coat the strips, 100 μl / well, place in a refrigerator at 4°C for 6-12 hours, then shake off the coating solution, wash once with washing solution, and then seal with blocking solution , 110 μl / well, and put in a refrigerator at 4°C f...
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