Novel coronavirus advantage epitope fusion protein, diagnosis reagent and application

Abstract

The invention relates to a novel coronavirus advantage epitope fusion protein, a diagnosis reagent and an application, and belongs to a viral diagnosis reagent. The novel coronavirus advantage epitopefusion protein comprises an N protein advantage epitope peptide and an S protein advantage epitope peptide which are connected through a connecting arm, and an E protein advantage epitope peptide, wherein the N protein advantage epitope peptide comprises one or two or above of an advantage epitope peptide N1, an advantage epitope peptide N2, an advantage epitope peptide N3 and an advantage epitope peptide N4. The novel coronavirus advantage epitope fusion protein is high in specificity, high in sensitivity (higher in positive detection rate), high in biocompatibility, high in solubility and good in stability, and is favorable for preparation of a corresponding detection reagent. The detection reagent prepared from the fusion protein is high in sensitivity and good in specificity, and early screening of novel coronaviruses can be realized.

Description

technical field [0001] The invention relates to the technical field of virus diagnostic reagents, in particular to a novel coronavirus dominant epitope fusion protein, diagnostic reagents and applications. Background technique [0002] Coronaviruses are a large family of viruses known to cause more serious illnesses such as colds, Middle East Respiratory Syndrome (MERS) and Severe Acute Respiratory Syndrome (SARS). The new coronavirus is a new strain of coronavirus that has never been found in humans before, and 2019-nCoV is the seventh type of coronavirus isolated from humans. Surveillance of related diseases revealed multiple cases of viral pneumonia, all of which were diagnosed as viral pneumonia / pulmonary infection. [0003] After the new coronavirus infects the human body, there is generally an incubation period of 3 to 14 days (up to 24 days in individual cases). During the incubation period, there may be no clinical symptoms and it is difficult to detect. Current ev...

AI Technical Summary

Problems solved by technology

After the virus infects the human body, it releases a large amount of spike protein (Spike protein, S protein) and nucleoprotein (Nucleoprotein, N protein) to stimulate the body to produce an immune response and produce antibodies against these two proteins. The produced diagnostic reagents will cause incomplete capture, and the sensitivity of missed detection is not high.

The use of two kinds of protein mixed coating will make the production process complicated, and the batch-to-batch control is difficult

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