Reagent kit for assessing endometrial receptivity and using method of reagent kit
A technology of endometrium and reagent kit, which is applied in the field of medical and biological detection, can solve the problems that morphological evaluation cannot accurately reflect the expression of endometrial cells and cell factors, and validity disputes, and achieve high-efficiency endometrial content Acceptability, efficient evaluation, and good stability
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Embodiment 1
[0046] Such as figure 1, the kit for evaluating endometrial receptivity in this embodiment, the kit includes a box body 1 and a first drawer-type compartment 2, a second drawer-type compartment 3 and a second drawer compartment arranged in the box body 1 Three drawer compartments 4, the first drawer compartment 1 is provided with a first foam cushion 801, the second drawer compartment 3 is provided with a second foam cushion 802, and the first foam cushion 801 Open 6 grooves that are respectively placed with gDNA scavenger buffer 9, gDNA scavenger 10, cDNA reverse transcription buffer 11, cDNA reverse transcriptase mixture 12, reverse transcription primer mixture 13 and the first enzyme-free water 14, said The first foam pad 801 offers a cavity for placing a 96-orifice plate 15 for reverse transcription, and the second foam pad 802 offers 7 grooves for placing probes 16, 1 groove for a positive control standard, and real-time Fluorescent quantitative polymerase chain reaction...
example
[0069] Example: By implementing fluorescent quantitative polymerase chain reaction, the Ct values of the positive control standard and the sample to be tested are obtained.
[0070]
[0071] By △△C T Calculate the relative expression of the target gene using the method (relative expression = 2 -△△Ct ):
[0072]
[0073] Correct the specific expression level by ActB and B2M, and take the mean of the two:
[0074]
[0075] That is, the relative expression levels of SLC2A1, HK2, PKM2, HIF1A, and SLC16A3 in the endometrium of patient 1 were 0.9709, 1.5306, 1.3419, 0.7378, and 1.1465 after correction. After correcting the data of SLC2A1, HK2, PKM2, HIF1A, and SLC16A3 in the endometrium of patient 2, the relative expression levels were 0.2630, 0.1914, 0.4690, 0.4729, and 0.3942, respectively.
[0076] Compared with the cut-off values of SLC2A1, HK2, PKM2, HIF1A, and SLC16A3 (0.5534; 0.6443; 0.6903; 0.7111; 0.6235), the relative expression levels of patient 1 were hig...
Embodiment 2
[0078] Such as figure 2 , the kit includes a box body (1) and a first drawer compartment (2), a second drawer compartment (3) and a third drawer compartment (4) arranged in the box body (1) ,
[0079] A first foam pad (801) is provided in the first drawer compartment (1), and a second foam pad (802) is provided in the second drawer compartment (3),
[0080] The first foam pad (801) has 6 sets of gDNA scavenger buffer (9), gDNA scavenger (10), cDNA reverse transcription buffer (11), cDNA reverse transcriptase mixture (12), reverse Record the groove of the primer mixture (13) and the first enzyme-free water (14), and the first foam pad (801) offers a cavity for placing the reverse transcription reaction using a 96-well plate (15),
[0081] The second foam pad (802) is provided with 7 grooves for placing the probe (16), 1 groove for placing the positive standard (17), and 1 groove for placing the real-time fluorescent quantitative polymerase chain reaction solution (19). Groo...
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