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3D gridding detection technique

A technology of three-dimensional grid and detection technology, applied in the field of three-dimensional grid detection technology, can solve the problems of insufficient sensitivity, high signal-to-noise ratio, and has not yet reached practical application.

Inactive Publication Date: 2007-05-09
河南省生物工程技术研究中心
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] However, these studies have basically not reached the ideal target, some sensitivity is not enough, some signal-to-noise ratio is too high, and some technical problems have not been solved, or are too complicated to be practically applied, etc.

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0081] Embodiment one hepatitis B virus genotyping

[0082] 1. Synthesis of Nucleotide Sequences Composing Grid Components

[0083] Artificially synthesized nucleotide sequences that make up the grid components, including A sequence (5'-aca caa cca cacact cta ctc a-3'), B sequence (5'-tct ctt ctc ctc tca tca act c-3'), A' sequence (5'-tga gta gag tgt gtg gtt gtg t-3') and B' sequence (5'-gag ttgatg aga gga gaa gag a-3') with mercaptopropane modification at its 3' end, the way is:

[0084] The synthesis and modification were all completed on a DNA synthesizer. The 3'-end alkylthiolated nucleotide sequence was synthesized using a 1-mercaptopropane-modified C3 S-S CPG solid-phase synthesis column and synthesized by a standard phosphoramidite chemical synthesis method. For purification, Do not remove the DMT protecting group. After the synthesis, the reaction column was incubated in concentrated ammonia water at 55°C for 16 hours, and 1ml of concentrated ammonia water was added...

Embodiment 2

[0112] Example 2 Coxsackie group B virus typing protein chip

[0113] 1. Synthesis of Nucleotide Sequences Composing Grid Components

[0114] Artificially synthesized nucleotide sequences that make up the grid components, including A sequence (5'-aca caa cca cacact cta ctc a-3'), B sequence (5'-tct ctt ctc ctc tca tca act c-3'), A' sequence (5'-tga gta gag tgt gtg gtt gtg t-3') and B' sequence (5'-gag ttgatg aga gga gaa gag a-3') with mercaptopropane modification at its 3' end, the way is:

[0115] The synthesis and modification were all completed on a DNA synthesizer. The 3'-end alkylthiolated nucleotide sequence was synthesized using a 1-mercaptopropane-modified C3 S-S CPG solid-phase synthesis column and synthesized by a standard phosphoramidite chemical synthesis method. For purification, Do not remove the DMT protecting group. After the synthesis, the reaction column was incubated in concentrated ammonia water at 55°C for 16 hours, and 1ml of concentrated ammonia water...

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Abstract

A 3D grid detection technique for detecting object nuclein and object protein includes requiring to add hybridized protein or hybridized probe on one of grid component when object nuclein or object protein is detected then coordinating by immobilized catch-protein or catch-probe to realize specificity-catch of object nuclein or object protein.

Description

technical field [0001] The invention relates to a three-dimensional grid detection technology, which can be applied in methods such as biochip, immunoassay, immunohistochemistry, transmembrane hybridization, etc., to detect target nucleic acid or target protein, and belongs to the technical field of biological detection. Background technique [0002] For purposes such as diagnosing diseases, screening new drugs, and scientific research, it is necessary to measure and analyze target nucleic acids or target proteins. In the current detection technology, some directly display the obtained results by the intensity or concentration of markers (such as the fluorescence intensity of biochip, the radiation intensity in radioimmunoassay, the concentration of colloidal gold in immunoassay, etc.), while others Indirectly display the results obtained in other ways (such as enzyme-catalyzed conversion of substrate color in immunoassays and immunohistochemistry, etc.). From the perspecti...

Claims

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Application Information

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IPC IPC(8): G01N21/78C12Q1/68G01N33/53C07H19/00
Inventor 李智涛王云龙陈小科李晨阳李玉林
Owner 河南省生物工程技术研究中心
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