Dot-immunogold filtration reagent kit for livestock schistosomiasis diagnosis, its preparation and application method

Abstract

The invention provides a preparing and application method of diffusion medium box for diagnosing livestock's snail fever spot gold, which is a new immune labeling technique and livestock's snail fever immunology diagnosis technique. The invention labels colloid gold onto Japanese snail antigen protein, and is suit for testing many kinds of animals such as cattle, sheep, pig and rabbit; it spots tested animal's blood dried paper leachate onto pyroxylin film as posited phase, then it droplets snail antibody colloid gold 2í½3 drops (100í½150ª–l) and produces naked eye visible red speckle in 2 minutes. Its positive coincidence rate is 100% and its negative coincidence rate is 99.2%.

Description

Technical field [0001] The present invention relates to the technical field of diagnosis of livestock schistosomiasis, in particular to a dot immunofiltration diagnostic kit for livestock schistosomiasis, and a preparation method and application method thereof. Background technique [0002] Domestic animal schistosomiasis not only harms the health of domestic animals, but also is an important spreader of its disease source. With the in-depth development of schistosomiasis control in our country, although great achievements have been made, there are still more than 100 counties (cities, districts) in our country that have failed to control the spread of the disease. At the same time, the disease has basically been eliminated in provinces and regions. There is still the task of long-term consolidation and monitoring. If the conventional fecal-hatched Schistosomiasis trichome method is used for detection, a large investment of manpower, material resources and...

AI Technical Summary

Problems solved by technology

The main problem of this method is that it is necessary to prepare schistosomiasis recombinant protein polyclonal antibody or rabbit anti-human IgG or rabbit anti-schistosome egg polyclonal antibody or GST-PcAb. The preparation of these specific polyclonal antibodies has a long cycle, complicated procedures, and high cost, which limits They are popularized and applied in the census of livestock schistosomiasis

Moreover, when preparing colloidal gold-labeled probes, the amount of polyclonal antibody is usually determined by visual inspection of the test tube. In order to ensure sufficient coating and avoid the interference of excess colloidal gold and non-specific protein binding to the detection result judgment, it is generally added on this basis. 10% to 20% of the polyclonal antibody dosage, and then in order to remove the excess free polyclonal antibody, purification such as chromatography or high-speed centrifugation is required. These treatments either require high equipment conditions, or the processing cycle is long, and the steps Complicated, so the cost is high, which is not conducive to industrial development; third, because the method uses the capture polyclonal antibody as the solid phase, and the serum to be tested and the colloidal gold probe as the mobile phase, one reaction box can only detect blood samples from one person , the detection cost is high and the efficiency is low; at the same time, the test box containing the nitrocellulose membrane coated with antigen or polyclonal antibody needs to be stored at 4-8°C, which increases the difficulty for large-scale application; the fourth is There are many reaction steps, and the interference is relatively increased