The invention discloses a
tissue engineering cartilage and a preparation method thereof. The
tissue engineering cartilage is cytoactive, consists of sequentially laminated
cell patches including a
surface layer, a middle layer, a deep layer and a calcified layer, and takes collagen matrix as a main component; the
layers are compounded by collagen and
hyaluronic acid, and the bordering integration of various
layers of
cartilage structures is promoted through hydrostatic culture; and the
tissue engineering cartilage is prepared by preparing various
layers of cartilage
cell patches and assembling multiple structural layers. The prepared tissue
engineering cartilage is in a layering arrangement of a natural cartilage, has a very good tissue structure and very good mechanical properties (
frictional coefficient is 75-85 percent that of a natural cartilage tissue, and the
elastic modulus is 80-90 percent that of the natural cartilage tissue.); the integration between different layers is good; experiments prove that the prepared tissue
engineering cartilage can form the tidal line same as that of a
normal cartilage tissue and has the same layering structure; and the prepared tissue
engineering cartilage is capable of promoting regeneration and
function recovery of a cartilage and obviously shortening the cycle of
cartilage damage treatment and is applicable to cartilage defect restoration for a load-bearing part of a
articular cartilage.