The invention discloses a color filter substrate, a liquid crystal display panel comprising the color filter substrate and a manufacturing method of the color filter substrate. The color filter substrate comprises a substrate body, black matrixes and color filter patterns, wherein the substrate body is provided with display regions and non-display regions located on the peripheries of the display regions, the black matrixes are arranged on the substrate body, sub-pixel regions are defined on the black matrixes, corresponding to the display regions, of the substrate body and the surface of the black matrixes, corresponding to the non-display regions, of the substrate body are uneven surfaces and the color filter patterns are arranged in the sub-pixel regions. According to the color filter substrate, the black matrixes, located at the positions of the non-display regions, on the color filter substrate are provided with the uneven surfaces, the shape of the face, making contact with sealant, of the color filter substrate is changed, the contacting area between the color filter substrate and the sealant is increased, the adhesion strength between the color filter substrate and the sealant is enhanced, the stripping strength of the color filter substrate and a thin film transistor array substrate is enhanced and the problem that liquid crystal leakage is generated due to the fact that the color filter substrate and the sealant are easy to strip is avoided.