Erythrocyte preserving fluid and application thereof

A technology for red blood cells and preservation solution, applied in the field of cell preservation solution, can solve the problems of loss of phospholipids, reduction of red blood cell viability, failure of preservation period to meet biological experiments and clinical needs, etc.

Active Publication Date: 2020-08-11
TIANJIN DEXIANG BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Decreased adenosine triphosphate (ATP) content also leads to reduced viability of red blood cells, loss of phospholipids, rupture of cell membranes, and release of contents, so that it cannot be used in various experiments
However, since the formula of these commercially available red blood cell preservation solutions has not been disclosed, researchers cannot use it to preserve the red blood cells they collect, especially some precious blood red blood cells
In addition, even if these preservation solutions can preserve red blood cells for about 90 days, the preservation period often still cannot meet the needs of biological experiments and clinical needs.

Method used

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  • Erythrocyte preserving fluid and application thereof
  • Erythrocyte preserving fluid and application thereof
  • Erythrocyte preserving fluid and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Preparation and washing of embodiment 1 erythrocytes

[0032] After the leukocytes were removed from 3 servings of fresh A-type healthy human suspension red blood cells purchased from Tianjin Blood Center, an appropriate amount of red blood cells was taken for mixing. Add 10 times the volume of sodium chloride injection, pipette to mix, place in a centrifuge and centrifuge at 3000rr for 5min, take out the centrifuged type A red blood cell test tube, suck off the supernatant, and repeat this process twice. After the third centrifugation, the supernatant was sucked off, and the remaining packed red blood cells were kept for future use. Type B packed red blood cells were prepared in a similar manner.

Embodiment 2

[0033] The preparation of embodiment 2 erythrocyte preservation solution

[0034] Prepare 6 groups of red blood cell preservation solutions according to the following ingredients.

[0035] (1) GMA preservation solution: adenine 1.8mmol / L; glucose 111mmol / L; mannitol 79.6mmol / L; sodium citrate 14mmol / L; MgCl 2 1.02mmol / L; Sodium chloride 23.32mmol / L; KH 2 PO 4 1.9mmol / L;Na 2 HPO 4 1.0mmol / L.

[0036] (2) Experimental group 1: adenine 1.8mmol / L, glucose 111mmol / L, mannitol 79.6mmol / L, sodium citrate 14mmol / L, MgCl 1.02mmol / L, sodium chloride 23.32mmol / L, KH 2 PO 41.9mmol / L, Na 2 HPO 4 1.0mmol / L, chloramphenicol 77.4mmol / L, neomycin sulfate 140.3mmol / L, EGCG 1μmol / L.

[0037] (3) Experimental group 2: the concentration of EGCG was changed to 5 μmol / L, and the concentration of other components was the same as that of experimental group 1.

[0038] (4) Experimental group 3: the concentration of EGCG was changed to 10 μmol / L, and the concentration of other components w...

Embodiment 3

[0041] The preparation of embodiment 3 erythrocyte suspension

[0042] According to the "Clinical Blood Transfusion Testing Operation Procedures", suck out 100 μL of the packed red blood cells prepared in Example 1 and add them into the dropper bottle filled with 10 mL of the red blood cell preservation solution prepared in Example 2 to obtain 0.8 % concentration of red blood cell suspension. For each red blood cell preservation solution, two bottles of red blood cell suspension were prepared, one bottle was used for detection, and the other bottle was used for static observation. Store in refrigerator at 2-8°C.

[0043] For the convenience of illustration, the erythrocyte suspension preserved in the GMA preservation solution is also referred to as "GMA control" in this paper, and the erythrocyte suspension preserved in the experimental group 1-5 preservation solution in Example 2 is correspondingly recorded as "#1" to "#5".

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Abstract

The present invention provides an erythrocyte preserving fluid comprising 5 to 15 [mu] mol / L of an epigallocatechin gallate (EGCG). The invention also provides a method for preserving erythrocytes andan erythrocyte suspension. The preservation period of the red blood cell suspension can be prolonged to 168 days by utilizing the red blood cell preservation solution.

Description

technical field [0001] The invention relates to a cell preservation solution, especially a cell preservation solution that can prolong the storage time of red blood cells. Background technique [0002] Red blood cells are often used in biological research and clinical practice, but the blood collection process is cumbersome and some precious blood collection opportunities are rare, so an effective red blood cell preservation solution is needed to maintain the activity of the collected red blood cells. When blood is stored at 2-8°C, over time, due to oxidative damage, the structure and function of the red blood cell membrane will change. Decreased adenosine triphosphate (ATP) content also leads to reduced viability of red blood cells, loss of phospholipids, rupture of cell membranes, and release of contents, so that it cannot be used in various experiments. [0003] Many laboratories use GMA red blood cell preservation solution (the main components include glucose (Glucose),...

Claims

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Application Information

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IPC IPC(8): A01N1/02G01N21/33G01N33/72G01N33/80
CPCA01N1/0215A01N1/0226A01N1/0221G01N33/80G01N33/721G01N21/33
Inventor 王丽王秀柱黄志刚王伟权商巧娜
Owner TIANJIN DEXIANG BIOTECHNOLOGY CO LTD
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