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Application of ganoderma lucidum polysaccharide in preparation of medicine for treating central nervous system injury and preparation method of ganoderma lucidum polysaccharide

A technology of the central nervous system and Lingsporin, which is applied in the field of medicine, can solve the problems of insufficient research and application in the field of neuroprotection, and achieve the effect of high content of active ingredients, high yield, and definite curative effect

Inactive Publication Date: 2021-11-19
山西国润制药有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Ganoderma lucidum spores are the reproductive cells of Ganoderma lucidum, which are rich in proteins, polysaccharides, amino acids, glycopeptides, vitamins, carotene, sterols, triterpenoids, alkaloids, fatty acids and other active ingredients, among which Polysaccharide is one of the main active ingredients. Modern studies have shown that polysaccharides have biological activities such as anti-tumor, anti-oxidation, and immune regulation, but insufficient attention has been paid to the research and application in the field of neuroprotection.

Method used

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  • Application of ganoderma lucidum polysaccharide in preparation of medicine for treating central nervous system injury and preparation method of ganoderma lucidum polysaccharide
  • Application of ganoderma lucidum polysaccharide in preparation of medicine for treating central nervous system injury and preparation method of ganoderma lucidum polysaccharide
  • Application of ganoderma lucidum polysaccharide in preparation of medicine for treating central nervous system injury and preparation method of ganoderma lucidum polysaccharide

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Experimental program
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Effect test

Embodiment 1

[0028] The preparation of lingosporin, its step is as follows:

[0029] (1) Ganoderma lucidum spores are broken, degreased with 1.5 times carbon tetrachloride, filter and collect the filter cake, then vacuum dry;

[0030] (2) Add 40 times of purified water by weight to the dried Ganoderma lucidum spore wall-broken powder, heat to 85° C. and decoct for 3 hours, centrifugally filter, and concentrate the supernatant into liquid extract. Add 100U / ml protease to the liquid extract, stir at room temperature for 1 hour, heat to inactivate the enzyme, cool down to room temperature, centrifugal filter and collect the supernatant.

[0031] (3) After concentrating the supernatant into a liquid extract, add medicinal ethanol so that the ethanol accounts for 85% of the total volume, filter after alcohol precipitation for 12 hours, collect the filter cake and dry the precipitate to obtain crude polysaccharide;

[0032] (4) Dissolve the crude polysaccharide in water, filter it with a tangen...

Embodiment 2

[0035] The preparation of lingosporin, its step is as follows:

[0036] (1) breaking the ganoderma spore wall, degreasing with 2 times of petroleum ether, collecting the filter cake by filtration, and then vacuum drying;

[0037] (2) Add 30 times of purified water by weight to the dried Ganoderma lucidum spore wall-broken powder, heat to 80° C. and decoct for 2 hours, centrifugally filter, and concentrate the supernatant into liquid extract. Add 90U / ml protease to the liquid extract, stir at room temperature for 1 hour, heat to inactivate the enzyme, cool down to room temperature, centrifugal filter and collect the supernatant.

[0038] (3) After concentrating the supernatant into a liquid extract, add medicinal ethanol so that the ethanol accounts for 90% of the total volume, filter after alcohol precipitation for 12 hours, collect the filter cake and dry the precipitate to obtain crude polysaccharide;

[0039] (4) Dissolve the crude polysaccharide in water, filter it with a...

Embodiment 3

[0042] The preparation of lingosporin, its step is as follows:

[0043] (1) breaking the ganoderma spore wall, degreasing with 2 times of petroleum ether, collecting the filter cake by filtration, and then vacuum drying;

[0044] (2) Add 40 times of purified water by weight to the dried Ganoderma lucidum spore-broken powder, heat to 90° C. and decoct for 2 hours, centrifugally filter, and concentrate the supernatant into a liquid extract. Add 80U / ml protease to the liquid extract, stir at room temperature for 1 hour, heat to inactivate the enzyme, cool down to room temperature, centrifuge and collect the supernatant.

[0045] (3) After concentrating the supernatant into a fluid extract, add medicinal ethanol so that the ethanol accounts for 80% of the total volume, filter after alcohol precipitation for 12 hours, collect the filter cake and dry the precipitate to obtain crude polysaccharide;

[0046](4) Dissolve the crude polysaccharide in water, filter it with a tangential f...

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Abstract

The invention relates to the technical field of medicines, in particular to application of ganoderma lucidum polysaccharide in preparation of a medicine for treating central nervous system injury, and discloses a preparation method of the ganoderma lucidum polysaccharide. The specific preparation method comprises the following steps: taking ganoderma lucidum spores as a raw material, and performing degreasing, water extraction, deproteinization, alcohol precipitation and drying to obtain ganoderma lucidum polysaccharide. Cell proliferation experiment detection shows that the ganoderma lucidum polysaccharide can promote neuronal cell proliferation and relieve neuronal damage caused by methamphetamine, and can improve mouse neurobehavioral disorder and learning and memory disorder caused by methamphetamine, reduce neuron loss and increase expression of nerve growth factors (BDNF). Therefore, the ganoderma lucidum polysaccharide has a good protection effect on neuron injury and can be used for treating central nervous system injury. According to the application of the ganoderma lucidum polysaccharide in preparation of the medicine for treating the central nervous system injury, the application field of the ganoderma lucidum polysaccharide is widened, and meanwhile, a new treatment scheme is provided for treatment of the central nervous system injury.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to the application of lingosporin in the preparation of medicines for treating central nervous system injuries, and also relates to a preparation method of lingsporean extract. Background technique [0002] Nerve cells are the most important type of cells in the nervous system, also known as neurons. They are the basic unit that constitutes the structure and function of the nervous system. Its main function is to receive stimuli and transmit information. A nerve cell is a type of endocrine cell that is composed of a cell body and cell processes that can transmit excitation. Nerve cells are essential constituent cells of the human body. Among them, sensory neurons can receive stimuli inside and outside the body and transmit nerve impulses to the central nervous system. Motor neurons pass through the neuron's cell body to the nerve endings that cause muscle contraction and glandula...

Claims

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Application Information

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IPC IPC(8): A61K31/715A61K36/074A61P25/00C08B37/00
CPCA61K31/715A61K36/074A61P25/00C08B37/0003
Inventor 杨育平王翰斌杨立志
Owner 山西国润制药有限公司
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