The invention discloses a
supercritical fluid extract technology of
ganoderma volatile oil, ganodenic acid and
ganoderma polysaccharide, which comprises the following steps: (1)
drying and crushing
ganoderma mycelium or ganoderma sporocarp; (2) adding ganoderma microbe
powder into the extraction cauldron of a supercritical CO2 fluid extracting
machine, setting the extracting pressure to be 15-25 MPa and the temperature to be 38-42 DEG C, leading 95-105L / min CO2 fluid, extracting for 1.5-2.5h, and obtaining the ganoderma volatile oil form a separation kettle; and (3) adjusting the extracting pressure to be 28-32 MPa and the extracting temperature to be 44-47 DEG C, taking
distilled water as the entrainer, extracting for 1.5-2.5h, obtaining the water solution containing the ganodenic acid and ganoderma
polysaccharide, adding 3.5-4.5 times of volume of 95% (V / V)
ethanol in the solution to generate flocculent precipitate, placing for 3-6h, centrifugalizing, separating, freezing and dryingthe precipitate, thus obtaining the ganoderma
polysaccharide product; decompression-concentrating the centrifugalized liquid supernatant to obtain the extract containing the ganodenic acid. The
supercritical fluid extract technology has simple operation, small
organic solvent usage, high extraction efficiency, high
recovery rate of active ingredients, good product quality and low production cost.