147 results about "Scale separation" patented technology

Monolithic cartridges including a plurality of nominally aligned polymer fibers can be used as stationary phase materials for liquid chromatography separations. Bundles of fibers are packed together so as to form capillary channels between the fibers. Different polymer compositions permit the “chemical tuning” of the separation process. The fibers can be physically or chemically bonded at spaced locations throughout the cartridge or can be packed together under pressure by use of an encasing wrap to form the capillary channels. Use of fibers allows a wide range of liquid flow rates with very low backpressures. Applications in HPLC, cap-LC, prep-scale separations, analytical separations, waste remediation / immobilization, extraction of selected organic molecules / ions from solution, purification of liquid streams (process waste, drinking water, pure solvents), selective extraction of cell matter and bacteria from growth media, and immobilization of cell matter and bacteria are envisioned.

A shadow mask method to fabricate electrodes with nanometer scale separation utilizes nanotubes (NTs). Metal wires with gaps are made by incorporating multi-wall carbon nanotubes (MWNTs) or single-wall carbon nanotubes (SWNTs) (or bundles thereof) into a tri-layer electron beam lithography process. The simple, highly controllable, and scaleable method can be used to make gaps with widths between 1 and 100 nm. Electronic transport measurements performed on individual SWNTs bridge nanogaps smaller than 30 nm. Metallic SWNTs exhibit quantum dot behavior with an 80 meV charging energy and a 20 meV energy level splitting. Semiconducting SWNTs show an anomalous field effect transistor behavior.

A spiral inertial filtration device is capable of high-throughput (1 mL / min), high-purity particle separation while concentrating recovered target particles by more than an order of magnitude. Large fractions of sample fluid are removed from a microchannel without disruption of concentrated particle streams by taking advantage of particle focusing in inertial spiral microfluidics, which is achieved by balancing inertial lift forces and Dean drag forces. To enable the calculation of channel geometries in the device for specific concentration factors, an equivalent circuit model was developed and experimentally validated. Large particle concentration factors were achieved by maintaining either average fluid velocity or Dean number throughout the entire length of the channel during the incremental removal of sample fluid. Also provided is the ability to simultaneously separate more than one particle from the same sample.

The present invention provides a method in which cDNAs that encode signal sequences for secreted or membrane-associated proteins are isolated using a fusion protein that directs secretion of a molecule that provides antibiotic resistance, e.g., .beta.-lactamase. The present method allows the isolation of signal peptide-associated proteins that may be difficult to isolate with other techniques. Moreover, the present method is amenable to throughput screening techniques and automation, and especially in validating the presence of the signal sequence via expression of the protein in both prokaryotic and eukaryotic cells. This invention provides a powerful and approach to the large scale isolation of novel secreted proteins.

A method which permits large-scale separation of a semiconducting carbon nanotube from a mixture of metallic and semiconducting carbon nanotubes based on differences in solubility resulting from preferentially reacting the metallic carbon nanotubes with an acid functional aryldiazonium salt to form a substantially fully functionalized metallic nanotubes which can be easily separated from the unfunctionalized semiconducting carbon nanotubes.

The invention discloses finished magnetic-particle extractant combined with magnetic nanometer microspheres, a preparation method thereof and application thereof in separation of radioactive species. The extractant consists of magnetic fine particles carrying separated functional groups of radioactive species. Nanometer particles of magnetism Fe3O4 are dispersed inside a polymer. A layer of ingredients selected from crown ether and ramification thereof or neutral phosphorus (TBP, TOPO) ramification and tertiary amine (TOA) ramification connected with chemical bonds is arranged on the surface of the polymer. The magnetic particle extractant can be directly added to solution containing radioactive species, and is stirred at a room temperature. The magnetic extractant is separated out with the applied magnetic field to separate radioactive species. The invention is integrated with the advantages of simple operation of magnetic separation and high selectivity of extraction separation, and can separate target radioactive species out from complicated radioactive liquid waste containing one or a plurality of kinds of <90>Sr and actinide elements. The magnetic-particles can be desorbed easily, and can be used repeatedly without producing secondary waste. In addition, by adopting the invention, continuous large-scale separation can be completed.

Polymer fibers having a novel cross-sectional geometry are used as stationary phase materials for liquid chromatography separations. Fibers of 20 to 50 micrometer diameters have surface-channel structures extending their entire lengths. Bundles of fibers having this novel cross-sectional geometry are packed in columns. Different polymer compositions permit the “chemical tuning” of the separation process. Channeled fibers composed of polystyrene and polypropylene have been used to separate mixtures of polyaromatic hydrocarbons (PAHs), Pb-containing compounds and fatty acids. Use of channeled fibers allows a wide range of liquid flow rates with very low backing pressures. Applications in HPLC, cap-LC, prep-scale separations, analytical separations, single fiber separations, waste remediation / immobilization, extraction of selected organic molecules / ions from solution, purification of liquid streams (process waste, drinking water, pure solvents), selective extraction of cell matter and bacteria from growth media, and immobilization of cell matter and bacteria are envisioned.

The invention discloses a deep preparation technology of coal for direct coal liquefacation. Feed coal is selectively crushed and then is sieved with a 0.5 mm particle size to grade; products passing through the sieving are separated through gravity concentration equipment, so as to obtain gravity treated cleaned coal, gravity treated middlings and gravity treated tailings; the gravity treated middlings is ground and fed into size mixing equipment; flotation reagent is added to the size mixing equipment, size mixing is performed, and then products are pumped into flotation equipment to perform separation, so as to obtain flotation cleaned coal and flotation tailings; the flotation cleaned coal is filtered and dried and then mixed with the gravity treated cleaned coal to form rich-vitrinite coal which is served as coal for direct coal liquefacation; and the products of the feed coal not passing through the sieving after crushing and sieving, the gravity treated tailings and the flotation tailings are mixed into rich-inertinite coal which can be served as high-quality steam coal, coal for gasification and the like. The technology is based on coal petrography maceral, adopts the differences in terms of densities and surface natures of different coal petrography maceral, and adopts the method of the combination of gravity concentration and flotation, so as to realize the large-scale separation and enrichment of coal maceral, increase the hydrogen-to-carbon ratio of coal for liquefacation, greatly improve the oil yield of direct liquefacation of coal, and obviously increase the economic benefit of direct liquefacation of coal.

The invention discloses a method for multiscale calculation of equivalent stiffness matrixes of complex composite material structures. The method comprises the following steps of: separating a macro-meso-micro three-scale structure by adoption of a scale separation method, and respectively establishing each scale analysis model according to geometric features of different scale models; and converting a three-scale problem into two multiscale problems: a macro-meso multiscale problem and a meso-micro multiscale problem, analyzing the two multiscale problems in sequence, and finally returning an equivalent modulus obtained by a micro multiscale problem to a macro multiscale problem. According to the method, the disadvantages such as low calculation efficiency and bad precision of the traditional structure analysis are overcome and the efficiency and precision of predicting performance of composite material structures are effectively enhanced; and the method can be used for instructing the work such as production, research and development of composite materials. The method can be applied to design and analysis of complex composite material structures in the field of aerospace, and can be applied to thermal and mechanical analysis problems of structure design in the engineering field of other composite materials.

The invention belongs to the field of sea surface wind direction inversion through a remote sensing means, and particularly relates to a navigation radar image sea surface wind direction inversion method based on a wave number energy spectrum. The method comprises the steps of preprocessing a radar image, extracting sea surface static characteristics and inverting sea surface wind direction information. According to the navigation radar image sea surface wind direction inversion method based on the wave number energy spectrum, a space domain does not need to be reduced, the space domain can automatically adapt to wind stripes of different scales, the wind direction can be measured under different sea conditions, an error result is stable, and engineering availability is high. According to the navigation radar image sea surface wind direction inversion method based on the wave number energy spectrum, only the wind stripe energy spectrum is extracted through wave number energy spectrum wave number scale separation, the wind direction inversion precision is improved, and the interference of the noise of other scales is reduced.

The invention provides a multi-scale calculation method for an equivalent heat conduction coefficient of a complicated composite material structure. According to the method, a scale separation method is adopted to separate a macro-scale structure, a meso-scale structure and a micro-scale structure, and all scale analysis models are established according to geometric characteristics of different scale models; a three-scale problem is converted into two multi-scale problems; a macro-meso multi-scale problem and a meso-micro multi-scale problem are analyzed in sequence, and an equivalent modulus obtained from a micro multi-scale problem is finally returned to a macro multi-scale problem. Through the method, the defects of low calculation efficiency and poor precision of traditional structure analysis methods are overcome, the efficiency and precision of structure performance prediction of a composite material are effectively improved, and the method can be used for guiding production, research and development and other work of the composite material. The method can be applied to complicated composite material structure design and analysis in the aerospace field and structural design thermal and mechanical analysis in other composite material engineering fields.

The invention provides a multi-scale calculation method for an equivalent thermal expansion coefficient of a complicated composite material structure. According to the method, a scale separation method is adopted to separate a macro-scale structure, a meso-scale structure and a micro-scale structure, and all scale analysis models are established according to geometric characteristics of different scale models; a three-scale problem is converted into two multi-scale problems; a macro-meso multi-scale problem and a meso-micro multi-scale problem are analyzed in sequence, and an equivalent modulus obtained from a micro multi-scale problem is finally returned to a macro multi-scale problem. Through the method, the defects of low calculation efficiency and poor precision of traditional structure analysis methods are overcome, the efficiency and precision of structure performance prediction of a composite material are effectively improved, and the method can be used for guiding production, research and development and other work of the composite material. The method can be applied to complicated composite material structure design and analysis in the aerospace field and structural design thermal and mechanical analysis in other composite material engineering fields.

The invention provides a synthesis method for a compound containing a mogrol derivative monomer (3) and a mogrol derivative monomer (4). Compared with an existing method for synthesizing the mogrol derivative monomer (3), the synthesis method is simple and low in cost, the novel mogrol derivative monomer (4) can be synthesized, and meanwhile on this basis, the invention further provides a method for preparing the mogrol derivative monomer (3) and the mogrol derivative monomer (4). According to the method, on the basis of the simple column chromatography separation technology, the mogrol derivative monomer (3) and the mogrol derivative monomer (4) can be prepared through low-cost, high-efficiency and large-scale separation, and a foundation is laid for further developing novel related medicines.

The invention relates to the field of biological medicines, in particular to a scale separation and purification method of pentaxanthin amylase. On the basis of the traditional extraction and separation method, The concentrating process of the invention comprises the flowing steps of: ultrafiltration concentration; chromatography by using a Cellulose DE52 ion exchange column; and then carrying out ultrafiltration concentration again; and chromatography by using a Sephacryl S-400HR gel filtration column; and finally removing impurities by dialysis. The invention puts emphasis on the separation and purification method of the pentaxanthin amylase and a scale purification technology thereof, not only can obtain high-yield and high-purity pentaxanthin amylase, but also provides a technological base for industrialized production.

A process for preparing the rigid high-molecular medium with ultra-large pores from calcium carbonate microparticles suspension includes preparing suspension of calcium carbonate, mixing glicide methylacrylate, benzoin ether, span-80 and ethanediol dimethylacrylate, dispersing said suspension in the mixed solution to become emulsion, emulsifying in the aqueous solution of polyvinyl alcohol, rotating while polymerizing reaction under ultraviolet irradiation, and washing with water and absolute alcohol. It can be used for chromatography.

The invention relates to a method for separating Aeromonas molluscorum producing tetrodotoxin from Takifugu fasciatus tissues and a fermentation culture method of the Aeromonas molluscorum as well as a detection method of the produced tetrodotoxin. The method for separating the Aeromonas molluscorum producing the tetrodotoxin from the Takifugu fasciatus tissues comprises a bacteria separation method, a bacteria identification method, a bacteria fermentation culture method, a competitiveness ELISA (Enzyme Linked Immunosorbent Assay) detection method and an LC-MS (Liquid Chromatograph-mass Spectrometry) detection method of the tetrodotoxin produced by the Aeromonas molluscorum, and the like. In the invention, by separating bacteria from Takifugu fasciatus ovaries and adopting a TCBS (Thiosulfate Citrate Bile (salt) Sucrose (agar)) culture medium for screening, the Aeromonas molluscorum capable of producing the tetrodotoxin is separated from blowfish bodies for the first time; the separated bacteria is identified by adopting a 16 SrDNA method; the competitiveness ELISA detection method and the LC-MS detection method are both firstly adopted for the tetrodotoxin produced by the separated Aeromonas molluscorum; and the produced tetrodotoxin and tetrodotoxin extracted from the blowfish bodies are determined to be the same substance; in addition, the separated Aeromonas molluscorum can massively separate the tetrodotoxin from the bacteria after being cultured.

This invention is related to a process for isolating and purifying lycopene, and more particularly to a process for large-scaled isolation and purification of the biosynthesized lycopene from bacterial cells via simple steps with high purity and recovery. The steps in the process of the present invention comprises: isolating a biomass from a fermented broth; treating the isolated biomass with alcohol; extracting the lycopene from the alcohol treated biomass with an organic solvent; removing the solid phase from the biomass / solvent extracting mixture; filtering the liquid-phase extract; crystallizing the lycopene by concentrating the liquid-phase extract; separating the dark red lycopene crystals; re-suspending the separated dark red lycopene crystals with acetone; and separating and drying the washed lycopene crystals. A biosynthesized lycopene purified by the process is also provided.

The invention relates to polyimide nano-fiber aerogel, a preparation method thereof and an application of the aerogel. The preparation method includes the steps: providing polyamide acid solution; preparing polyamide acid nano-fibers from the polyamide acid solution by an electrostatic spinning method; dispersing the polyamide acid nano-fibers in liquid, and freeze-drying the liquid containing thepolyamide acid nano-fibers to obtain the polyamide acid nano-fiber aerogel; performing imidization on the polyamide acid nano-fiber aerogel to obtain the polyimide nano-fiber aerogel. The polyimide nano-fiber aerogel has excellent mechanical flexibility, efficient oil-water separation for different oil and organic solvents, antifouling and self-cleaning characteristics and high reusability. A preparation process of the aerogel is simple and easy to implement, and raw materials are rich in source and low in cost. The aerogel can be used for the fields of large-scale separation and purificationof industrial oil and water (oil-water emulsion), large-scale filtration and separation of organic liquid / water and the like.

The invention discloses artificially modified high-activity Mariner-Like transposase. Active transposase cloned from moso bamboo and plant MLE (Mariner-Like Elements) transposase with higher activity obtained after molecular optimization of the active transposase lay a foundation for developing genetic labels by means of the MLE transposase and provide a powerful guarantee for large-scale separation and marking of gene functions in the gene function.

The invention discloses a contact characteristic analysis method based on three-dimensional microscopic surface morphology, and is suitable for researching the contact rigidity and the contact dampingof the combined surface based on the multi-scale theory. According to the method, the surface morphology of the combined surface is obtained through a three-dimensional morphology measuring instrument, and multi-scale decomposition reconstruction is carried out through wavelet transformation, and a three-dimensional entity model for the data of separated different frequency bands. Due to the factthat the established contact model is based on real surface data and influences of different scales are considered, the accuracy of the contact model is improved to a great extent, and the research of the contact characteristic of the combined surface is closer to the actual situation. The real surface morphology height matrix is subjected to multi-scale separation by wavelet transformation, andthe three-dimensional entity model for the data of the separated different frequency bands. Due to the fact that the established contact model is based on real surface data and influences of differentscales are considered, the accuracy of the contact model is improved to a great extent.

The invention discloses a heparin affinity column as well as the preparation method and the application thereof. The heparin affinity column is prepared through the following method: agarose gel 6FF is taken as a solid phase carrier which is firstly activated and then is aminated and coupled with amidocyanogen after an epoxy group is coupled, the aminated agarose gel 6FF and the heparin form intermediate aldimine in methanol, and the intermediate product of aldimine is further subjected to reductive amination for forming stable chemical bonds, thereby acquiring the heparin-agarose gel 6FF. The affinity column preparing preparation with high efficiency is simple and short. Through adopting the combination of the rear aldehyde of the heparin and the amidocyanogen of aminated sepharose, the activity of the heparin is not influenced, thereby ensuring the affinity column to have higher affinity; the heparin coupling is stable, and the heparin can be repeatedly used; the reagent adopted by the method has low cost and no environmental pollution, thereby laying a foundation for sweepingly isolating and purifying materials with specific binding capacity to the heparin in common laboratories, and promoting the research on domestic relative fields.

The invention belongs to the field of time domain calculation electromagnetism, and relates to an electromagnetism multi-scale calculation method based on a hybrid grid and a time step length. According to the method, a multi-scale time domain electromagnetic problem is decomposed into a macro scale part and a micro scale part, scale separation is achieved, the solving scale of an original problemis effectively reduced, a mixed grid suitable for the characteristics of a micro unit is adopted in the micro unit for discretization, and the solving freedom degree of the micro unit is further reduced. And in the aspect of time iteration, independent time step lengths are adopted for different microcosmic units for time discretization, so that the defect of too many times of time iteration caused by too small local microgrid size is effectively avoided. After the microscopic information is solved through the microscopic problem, coupling between the macroscopic problem and the microscopic problem is achieved, the macroscopic problem is substituted into the macroscopic problem, iteration solving is conducted through the macroscopic time step length, and finally the technical effect of efficiently solving the multi-scale time domain electromagnetic problem is achieved.

The invention discloses a powder metering control system, method and device. The impact of air flow in an agitating host machine in the powder metering process to power metering is effectively eliminated, so that negative pressure in the metering process is fundamentally avoided. The system comprises a powder scale, the agitating host machine, a powder scale butterfly valve between the powder scale and the agitating host machine, and a powder scale separation butterfly valve between the powder scale butterfly valve and the agitating host machine.

The invention discloses a self-suction multiple-stage centrifugal pump with a variable-scale separation net. The self-suction multiple-stage centrifugal pump comprises the variable-scale annular separation net and an annular net frame. The variable-scale separation net and the net frame are closely attached together to be mounted between a centrifugal pump outlet and a backflow hole and are closeto the centrifugal pump outlet. The scales of grids, in the circumferential direction of the separation net, of the separation net change constantly. According to the specific change rule, smaller-scale and closer separation nets are distributed in areas high in bubble concentration and axial speed, bubble backflow is stopped, and bubble floating is accelerated; and larger-scale and sparser separation nets are distributed in areas small in bubble concentration and low in axial speed, backflow flowing resistance of the self-suction stage is reduced, and self-suction is accelerated. According tothe self-suction multiple-stage centrifugal pump, turbulent flow in a pump casing cavity at different rotating speeds is fully rectified, flowing uniformity is obviously enhanced after the flow passes through the variable-scale separation net, the fluid speed is reduced, and pressure is enhanced; and fluid kinetic energy can be converted into pressure potential energy better, and the pressure difference between an inlet cavity and an impeller inlet is increased.