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Clustering enzyme with atomic precision, preparation and application of clustering enzyme in nervous system diseases

A cluster and precision technology, applied in the field of medicine, can solve the problems of lack of effective treatment measures for nervous system diseases, and achieve the effect of promoting wound healing and weight recovery

Active Publication Date: 2021-12-24
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to solve the problems in the prior art, the present invention provides an atomic precision cluster enzyme and its preparation and its application in nervous system diseases, so as to solve the problem that many nervous system diseases still lack effective treatment measures in the prior art

Method used

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  • Clustering enzyme with atomic precision, preparation and application of clustering enzyme in nervous system diseases
  • Clustering enzyme with atomic precision, preparation and application of clustering enzyme in nervous system diseases
  • Clustering enzyme with atomic precision, preparation and application of clustering enzyme in nervous system diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] This example prepares an atomic precision cluster enzyme. Its preparation method comprises the following steps:

[0038] (1) In proportion, 1.7mg HAuCl 4 ·3H 2O was dissolved in 0.25mL deionized water to obtain solution one; 1.06mg MPA was dissolved in 2mL deionized water to obtain solution two; 16mg sodium hydroxide was dissolved in 0.4mL deionized water to obtain solution three; 0.1mL solution three was added Dilute with 0.4mL of deionized water, add 2.15mg of sodium borohydride and mix to obtain solution 4; at room temperature, add the above solution 1 and solution 2 to 2.35mL water in turn, stir for 5min, then add 0.3mL of solution 3 and 0.1 Four mL of solution, sealed, and reacted in the dark for 3 hours to obtain a brown solution, stop the reaction, place the reaction solution at 4°C for 12 hours, use 3K and 10K ultrafiltration tubes to purify by ultrafiltration at 3500rpm / min, freeze-dry, Au to get MPA ligand 25 cluster enzyme.

[0039] (2) In proportion, 1....

experiment example 1

[0041] The prepared cluster enzyme was subjected to the following characterization tests:

[0042] (1) Au 25 MPA 18 The crystal structure of cluster enzyme (prepared in Example 1), such as figure 1 As shown, it can be seen that: Au 25 MPA 18 The structure of the cluster is composed of 25 Au atoms and 18 MPA ligands, and has a unique cage structure, in which the cluster enzyme doped with different atoms is only Au 25 One Au atom in the oligomer motif site in the structure was replaced with a Cu or Cd atom.

[0043] (2) ESI-MS mass spectrometry characterization of cluster enzyme (prepared in Example 1), the results are as follows figure 2 As shown in a, it can be seen from the figure that: Au 25 MPA 18 There is the highest molecular ion peak at m / z≈2271, which can be assigned to [Au 25 MPA 18 -3H] 3- . The mass-to-charge ratio of the most abundant molecular ion peak decreases after atom doping, and the Cu atom-doped cluster has the highest molecular ion peak at m / z≈2...

experiment example 2

[0046] Cluster enzyme (prepared in embodiment 1) is carried out catalytic activity test:

[0047] (1) Total antioxidant performance test: According to the specific test method in the instruction manual of the ABTS rapid method kit, the total antioxidant capacity of cluster enzymes is measured, and compared with the total antioxidant capacity of natural antioxidants Trolox and anthocyanins ,Such as image 3 as shown in a. Au substituted by Cu atoms and Cd atoms 24 Cu 1 and Au 24 CD 1 Has super antioxidant capacity, compared to Au 25 It has increased by 41 times and 48 times, respectively 137 times and 160 times that of natural antioxidant Trolox, and 7.5 times and 9 times that of anthocyanins.

[0048] Experiments have proved that the cluster enzymes prepared in Examples 1 and 2 have super high antioxidant activity beyond natural antioxidants.

[0049] (2) Determination of enzyme activity:

[0050] CAT-like enzyme activity can utilize H 2 o 2 Unique optical absorption...

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Abstract

The invention discloses a clustering enzyme with atomic precision, preparation and application of the clustering enzyme in nervous system diseases. The clustering enzyme is prepared by the following method: preparing HAuCl4 water as a solution I; 3-mercaptopropionic acid aqueous solution as a solution II; adding the solution I into the solution II, and stirring to obtain a colorless and transparent mixed solution; continuously adding a NaOH aqueous solution and a NaBH4 solution into the reaction system; and sealing and reacting in a dark place at room temperature to obtain a brown solution, stopping the reaction, and aging the reaction solution to obtain the final clustering enzyme. The research finds that the clustering enzyme with atomic precision has ultrahigh antioxidant activity and enzyme-like selectivity, is used for early intervention of mouse nervous system diseases, and can remarkably promote wound healing and weight recovery and effectively improve later behavioral characteristics. Important reference and basis are provided for research and development of medicines for treating nervous system diseases.

Description

technical field [0001] The invention belongs to the field of medicine and relates to an artificial enzyme, in particular to an atomic precision cluster enzyme, a preparation method and its application in early intervention of nervous system diseases. Background technique [0002] Neurological disorders refer to a small group of medical conditions that affect the nervous system. The category includes more than 600 different conditions, including genetic disorders, infections, cancers, epilepsy, cardiovascular diseases (such as stroke), congenital and developmental disorders (such as spina bifida), and degenerative diseases (such as multiple sclerosis, Alzheimer's disease, Parkinson's disease, and amyotrophic lateral sclerosis). Its symptoms may involve other organ systems and cause motor dysfunction, sensory disturbances, pain, etc. According to the Global Burden of Disease report, neurological diseases are one of the leading causes of death and disability worldwide. As po...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K33/242A61P25/28A61P25/00A61P17/02C07C323/52C07F19/00
CPCA61K33/242A61P25/28A61P25/00A61P17/02C07C323/52C07F3/003Y02A50/30
Inventor 张晓东刘海乐张晓宁黄优焦萌露
Owner TIANJIN UNIV
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