45 results about "Artificial enzyme" patented technology

Provides an artificial enzyme obtained by improving upon a sequence of a natural nicotine dehydrogenase, wherein the improvement comprises replacing at least one amino acid hindering product release with an amino acid with smaller side chains, thereby improving a catalytic rate.

The invention discloses a method for efficiently producing chondroitin sulfate A by artificial enzyme method, which belongs to the technical field of bioengineering. This method expresses a bifunctional protein chondroitin 4‑O‑sulfotransferase C4ST and aryl sulfotransferase AST IV through microbial heterologous expression, which is used to catalyze the synthesis of chondroitin sulfate A from chondroitin, combined with site-directed mutagenesis and The optimization of the components of the reaction system accelerates the synthesis efficiency of chondroitin sulfate.

Production of transparent acid containing tellurium with high glutathione oxide enzyme activity is artificial enzyme process with GPX activity. It consists of transparent acid activating, tellurium sodium hydride preparing, transparent acid tellurium reacting, tellurium transparent acid oxidizing and after processing. The production is carried out by activating transparent acid with tolylsulfonyl chlorin, preparing transparent acid benzene sulfonyl ester, dialyzing in distilled water, adding tellurium powder and sodium borohydride into anhydrous alcohol, heating and reverse flowing in nitrogen system, adding anaerobic glacial acetic acid, preparing alcohol solution of tellurium sodium hydride preparing, alcohol of tellurium sodium hydride reacting with transparent acid benzene sulfonyl ester solution, obtaining transparent acid containing the, the reacting system expose in air, centrifugal oxidizing, alcohol settling, and washing. It achieves simple process, high output, higher resistance to oxidation and GPX activity.

A method for improving peroxidase-like activity of nanozyme and a product thereof are disclosed, which relate to the field of artificial enzymes in biochemistry. The method adopts a hydrogen peroxide solution with high concentration to treat the VO2(B) powder for obtaining a product with high peroxidase-like activity. Compared with the pure VO2(B) powder, the peroxidase-like activity of the product obtained by the method is increased by 4 to 12 times. The method provided by the present invention adopts raw materials with low cost and mild reaction conditions, is simple in operation and low in cost, which is conducive to batch preparation. The powder product obtained by the method is able to be applied to detect hydrogen peroxide, glucose, etc., and has great application prospects in biosensing, industrial wastewater treatment and sewage treatment.

The invention provides a glutathione peroxidase GPX2 mutant with high activity and a preparation method thereof, and belongs to the field of biotechnology. According to the invention, the artificial GPX2 is used as a template to obtain a novel glutathione peroxidase GPX2 mutant with high activity through computer simulation and site-specific mutagenesis. The glutathione peroxidase GPX2 mutant with high activity comprises 203 amino acids; compared with artificial GPX2, the glutathione peroxidase GPX2 mutant with high activity does not contain cysteine and has higher GPX activity and better stability and enables, and all cysteines in the artificial GPX2 are enabled to be mutated into serine, but selenocysteine (SeCys) still is in the 40th site. According to the preparation method, based on a genetic engineering technology, the GPX2 mutant protein is directly expressed in mammal cell strains or an auxotroph prokaryotic expression system and an SPP low-temperature expression system; the novel artificial enzyme with high GPX2 activity can be prepared without chemical modification; the method provided by the invention has a wide application perspective in bio-pharmaceuticals.