123 results about "Selenocysteine" patented technology

The five component composition consisting essentially of: (1) Water soluble antioxidant vitamin C or ascorbic acid, or any of its forms or derivatives, or mixtures thereof. (2) Oil soluble antioxidant vitamin E or Alpha-tocophorol, or any of its forms or derivatives, or mixtures thereof. (3) The element selenium, or a chemical (or composition) containing it, or mixtures thereof. The most preferred chemical containing selenium is dimethyl selenide and mixtures thereof. The words "dimethyl selenide" here and hereinafter mean dimethyl selenide and / or it's oxidation products, including dimethyl selenoxide. (4) A sulfur amino acid, in any form, or a sulfur peptide, or a sulfur protein, or any of their derivatives, or mixtures thereof. The mixture of methionine and cysteine, which contains as impurities some seleno-methionine and some selenocysteine, is preferred,-the tripeptide glutathione containing cysteine is also preferred. (5) Another antioxidant, other than vitamin C and other than vitamin E, which is synthetic or natural and water soluble or oil soluble, or a mixture of such antioxidants, or a combination of such forms thereof. The mixtures of butylated hydroxyanisole and ethoxyquin is preferred.

The invention provides methods and compositions employing hybrid molecules of a synthetic molecule and antibody or antibody fragment comprising a selenocysteine residue, wherein the synthetic molecule is covalently linked to the antibody or antibody fragment at the selenocysteine residue. The invention also provides a composition comprising a hybrid molecule as described above and a pharmaceutically acceptable carrier. The invention further provides for methods of making the hybrid molecules, and methods of using the hybrid molecule described above to inhibit cell surface receptor binding.

Non-naturally occurring tRNASec and methods of using them for recombinant expression of proteins engineered to include one or more selenocysteine residues are disclosed. The non-naturally occurring tRNASec can be used for recombinant manufacture of selenocysteine containing polypeptides encoded by mRNA without the requirement of an SECIS element. In some embodiments, selenocysteine containing polypeptides are manufactured by co-expressing a non-naturally occurring tRNASec a recombinant expression system, such as E. coli, with SerRS, EF-Tu, SelA, or PSTK and SepSecS, and an mRNA with at least one codon that recognizes the anticodon of the non-naturally occurring tRNASec.

The invention discloses selenium-rich feed for cultivating selenium-rich fish, which is characterized in that the content of total selenium in the selenium-rich feed is 1.5-20mg / kg, wherein selenomethionine accounts for 60%-80% of the total selenium, and selenocysteine accounts for 1%-10% of the total selenium. The selenium content of the selenium-rich fish cultivated by the selenium-rich feed is stable and controllable (the meat selenium content can reach to 1.5-10.0mg / kg, and the proportion of organic selenium is larger than or equal to 80 %), the selenium is safe in form and good in absorption effect, and patients lack of the selenium can improve the condition of lack of the selenium in a short time by eating the selenium-rich fish. In addition, a selenium-rich method is easy to popularize, and large-scale production can be achieved.

The invention relates to a detection method of selenium form in an aquatic product, and belongs to the technical field of aquatic product detection. The selenium form in the aquatic product is extracted by oscillating with a super constant-temperature mixer and measured by a high performance liquid chromatography-hydride generation atomic fluorescence spectroscopy combined morphological analysis technology, the extracting solution is separated by an anion chromatographic column, different forms of selenium are eluted successively by a moving phase, hydrogenation is carried out on the elution solution through a potassium borohydride reducing agent and hydrochloric acid to generate a hydride which enters an atomizer and is used in combination with atomic fluorescence for analysis and measurement, so that a more complete biological property of the selenium is provided. The experiment condition is reasonable, the detection data are accurate and reliable, the sample recovery rate is 88.79-94.88% and the degree of precision is less than 5%. The method is high in sensitivity, low in interference and simple in sample pretreatment, can be used for accurately measuring inorganic selenium, selenite and selenate as well as organic selenium, selenocysteine and selenomethionine and detecting selenium form in aquatic product.

Convenient processes are described for the synthesis of L-Se-methyselenocysteine from chloroalanine derivatives. The process is easily extendable to other selenium substituted amino acids. DL-Se-methyselenocysteine is easily obtained by a benzaldehyde-catalyzed racemization of L-Se-methyselenocysteine.

The invention discloses a novel antitumor medicament, in particular a benzisoselenazolone difluorocytidine compound or a pharmaceutically acceptable salt or ester thereof as well as a preparation method and application thereof, wherein a benzisoselenazolone structure can identify selenocysteine residues in the activity center of thioredoxin reductase to generate the action of reversibly competitive inhibition and reserves the activity structure of 2'-deoxidization-2'2'-difluorocytidine. Therefore, the benzisoselenazolone difluorocytidine compound in the invention can selectively kill tumor cells, inhibit the growth of the tumor cells, and has the characteristics of high efficiency and low toxicity.

The present invention provides a compound having the structure:A-B-----Zwherein A is a biologically active structure of the compound; wherein Z is a protein component of the compound, which protein component comprises one or more polypeptides, wherein at least one of the one or more polypeptides comprises consecutive amino acids which (i) are identical to a stretch of consecutive amino acids present in a chain of an Fc domain of an antibody; (ii) bind to an Fc receptor; and (iii) have at their N-terminus a sequence selected from the group consisting of a cysteine or selenocysteine; wherein the dashed line between B and Z represents a peptidyl linkage; and wherein the solid line between A and B represents a nonpeptidyl linkage, as well as intermediates dimers thereof, and processes of producing the compounds of the invention.

The invention discloses mutton rich in organic selenium and a production method thereof as well as an adopted concentrated feed rich in organic selenium. The selenium content of the mutton rich in organic selenium is 200-1000 microgrammes / kg, wherein the content of the organic selenium is more than or equal to 85%. The method comprises the following steps of: selecting a healthy meat sheep under the fattened early stage; feeding 200-1000 microgrammes / kg of total selenium each day; and feeding with the concentrated feed of which the selenomethionine accounts for 60-80% of the total selenium ratio and the selenocysteine accounts for 1-10% of the total selenium ratio until fattening. The meat of the meat sheep fed with the method is the mutton rich in organic selenium. The mutton rich in organic selenium, which is disclosed by the invention, has the advantages of stable and controllable selenium content, safer form and better adsorption effect, the selenium enriching method is easy to popularize, and large-scale production can be carried out.

Non-naturally occurring tRNASec and methods of using them for recombinant expression of proteins engineered to include one or more selenocysteine residues are disclosed. The non-naturally occurring tRNASec can be used for recombinant manufacture of selenocysteine containing polypeptides encoded by mRNA without the requirement of an SECIS element. In some embodiments, selenocysteine containing polypeptides are manufactured by co-expressing a non-naturally occurring tRNASec a recombinant expression system, such as E. coli, with SerRS, EF-Tu, SelA, or PSTK and SepSecS, and an mRNA with at least one codon that recognizes the anticodon of the non-naturally occurring tRNASec.

Disclosed is a novel synthetic method for isomeric peptides through an appropriate linkage of L-selenomethionine or Se-Methyl-L-selenocysteine with L-glutamic acid. The novel synthetic method produces isomeric peptides of L-selenomethionine or Se-Methyl-L-selenocysteine that exhibit (i) enhanced water solubility; (ii) enhanced rate of dissolution in water; (iii) enhanced bioavailability; (iv) excellent vascular endothelial growth factor promoting activity; (v) excellent anti-5-alpha-reductase activity; (vi) capabilities to prevent / reduce “hair fall” and promote “hair growth”, thereby maintaining a perfect homeostasis for “hair care”. Cosmeceutical and pharmaceutical compositions comprising the isomeric peptides obtained through an appropriate linkage of L-selenomethionine or Se-Methyl-L-selenocysteine with L-glutamic acid are also disclosed. Other dipeptides with several other amino acids and uses thereof are also disclosed.

The invention discloses a planting method of selenium-rich bananas. The planting method comprises the steps of pretreatment of soil, wherein the soil is sterilized with an ultraviolet germicidal lamp and fertilized with selenium-rich organic fertilizer, planting, sunshading to protect seedlings, fertilization and management, intercropping, banana plant infusion, wherein at the beginning of August, a hole is processed in banana plant cauloid base portions downward in a inclined mode at 45 degrees, the liquid outlet of an infusion bottle is connected to an infusion tube, a plug on the other end of the infusion tube is inserted into the processed hole, the infusion bottle is suspended on a banana plant, and the infusion bottle is filled with selenium-rich amino acid nutrition liquid which is prepared from, by weight, 3-5 parts of selenocystine, 2-4 parts of selenomethionine, 4-5 parts of selenocysteine, 5-7 parts of methylselenocysteine, 6-8 parts of selenohomocysteine, 3-5 parts of honey, 2-3 parts of selenosulfate and 300 parts of water, and pest and disease control. The planting method can improve the survival rate of the banana plant, promote absorption of bananas to selenium elements, increase the selenium content of the bananas and remarkably improve the economic benefits of planting bananas.

The invention discloses a duck feed rich in organic selenium. If the feed is fed from young ducks, the production performance, the meat selenium content and the duck meat quality of meat ducks can be effectively improved. The total selenium content of the duck feed rich in organic selenium is 300-1000 microgrammes / kg, wherein selenomethionine accounts for 60-80% of the total selenium ratio, and selenocysteine accounts for 1-10% of the total selenium ratio. According to the feed, the immunity, the production performance, the meat selenium content and the duck meat quality of the meat duck can be effectively improved, and the duck feed can be widely applied to the cultivation of the meat duck.

The invention discloses a production method for beef rich in organic selenium and a concentrated feed rich in organic selenium. The selenium content of the beef rich in organic selenium is 200-1000 microgrammes / kg, wherein the content of the organic selenium is more than or equal to 85%. The method comprises the following steps of: selecting a healthy meat cattle under the fattened early stage; feeding 200-1000 microgrammes / kg of total selenium each day; and feeding with the concentrated feed of which the selenomethionine accounts for 60-80% of the total selenium ratio and the selenocysteine accounts for 1-10% of the total selenium ratio until fattening. The meat of the cattle fed with the method is the beef rich in organic selenium. The beef rich in organic selenium, which is disclosed by the invention, has the advantages of stable and controllable content, safer form and better adsorption effect, the selenium enriching method is easy to popularize, and large-scale production can be carried out.

The invention discloses a selenium-enriched cardamine hupingshanesis plant material and a cultivation method for the same, wherein selenium in the plant material exists in the form of selenocysteine, and the content of the selenium in the form of selenocysteine in the plant material is 0.2-20000 mg / kg and occupies more than 90% of the total content ratio of selenium. The selenium-enriched cardamine hupingshanesis plant material is cultivated according to a conventional method, wherein the conventional method comprises the following steps of: adding a selenium source in the growth medium of cardamine hupingshanesis during the growth cycle of the cardamine hupingshanesis; adjusting the content of selenium in the growth medium to achieve 0.1-1000 mg / kg; and adding alkaline substances simultaneously to keep the pH value of the overall or local environment of the growth medium in a range from 7 to 8. The material can be used as good selenium source supplementary substance for human body and animals, and directly eaten or used as selenium-enriched food or selenium-enriched feed beneficial to the health of human and animals.

The invention discloses a whitening anti-aging skin softening lotion and a preparation method thereof. Active ingredients in the skin softening lotion include, by weight, 0.1-1 part of hirudin, 0.2-0.4 part of astaxanthin, 2-3 parts of tranexamic acid, 0.01-0.05 part of melatonin, 4-6 parts of glutathione, 1-3 parts of alpha-arbutin, 0.6-1 part of resveratrol, 0.1-0.2 part of gallogen, 0.1-0.5 part of selenium cysteine, 0.02-0.4 part of GSH-Px, 0.2-0.8 part of SOD, 0.6-2 parts of vitamin C ethylether, 0.4-0.6 part of nicotinamide, 2-4 parts of carnosine and 1-2 parts of green plum extract. Theskin softening lotion inhibits activity of tysocinase in melanoblast and cut off synthesis path of melanin, then further inhibits dopamine peroxidation, reduces melanin and delay skin cell aging; selenium cysteine and GSH-Px are utilized to collaboratively catalyze to participate in peroxidation reaction, so that whitening and anti-aging effects are further enhanced, and the skin softening lotionhas good moisturizing performance.

The invention belongs to the field of chemical synthesis, and concretely relates to a synthetic method for L-selenocysteine. The method comprises the following steps: a, chloridizing L-serine hydrochloride to obtain 3-chloro-L-alanine hydrochloride; b, performing seleno-reaction of 3-chloro-L-alanine hydrochloride prepared by step a under alkaline condition to obtain L-selenocystine; and c, performing reduction reaction of L-selenocystine to obtain L-selenocysteine. The method has simple steps, high yield, low cost, and good application prospect.

HIV-1 is a slow virus that has a genome that requires the removal of glutathione, glutathione peroxidase and of selenium, cysteine, glutamine and tryptophan from its host as it is replicated. Although various clinical trials have improved the health of AIDS patients by correcting one or more of these nutritional deficiencies, they have never been addressed together. The current invention combines selenium, cysteine, tryptophan, and glutamine in powder, capsule, or pill form for the creation of glutathione and glutathione peroxidasae by the patient and the correction of deficiencies of other biochemical substances that require selenium, cysteine, tryptophan, and glutamine as precursors. Such a treatment protocol will prevent HIV-1 seropositive individuals from developing AIDS and reverse most of the symptoms of AIDS in those with this disorder.

The invention relates to a selenocysteine near-infrared fluorescent probe, and a preparation method and application thereof, belonging to the field of chemical analysis and detection. The near-infrared fluorescent probe Fsec-3 for detecting selenocysteine provided by the invention has the advantages of good selectivity, high sensitivity, low detection limit (20 nM) and good biocompatibility. In aphosphate buffer, the fluorescence intensity and the concentration of selenocysteine (0-100[mu]M) are in a good linear relationship, which indicates that the probe Fsec-3 is suitable for quantitativedetection of selenocysteine. The probe Fsec-3 achieves fluorescence imaging of selenocysteine in the human breast cancer cell MCF-7; and the probe Fsec-3 also achieves sensitive detection of endogenous selenocysteine in a living body, and is fast in response and stable in fluorescence signals. Thus, the fluorescent probe Fsec-3 prepared by the invention is an effective tool for visual and quantitative detection of the content of selenocysteine in cells, living bodies and tumor tissue.

The present invention relates to methods for the chemo-selective pegylation of the cysteine residue having unoxidized sulfhydryl side-chain and free α-amino group in proteins, peptides and other molecules. Similar methods are provided for the chemo-selective pegylation of the homocysteine, selenocysteine, penicillamine, and N-methyl-cysteine residues.

Non-naturally occurring tRNASec and methods of using them for recombinant expression of proteins engineered to include one or more selenocysteine residues are disclosed. The non-naturally occurring tRNASec can be used for recombinant manufacture of selenocysteine containing polypeptides encoded by mRNA without the requirement of an SECIS element. In some embodiments, selenocysteine containing polypeptides are manufactured by co-expressing a non-naturally occurring tRNASec a recombinant expression system, such as E. coli, with SerRS, EF-Tu, SelA, or PSTK and SepSecS, and an mRNA with at least one codon that recognizes the anticodon of the non-naturally occurring tRNASec.

The invention discloses a feed additive for laying hens and application of the feed additive for the laying hens. Through use of feed additive, the function of the laying hens is not damaged, the taste of chicken is not changed and adverse effect on the laying hens are not generated while high-selenium, high-zinc and high-iron eggs can be obtained. The feed additive for the laying hens comprises an additive I composed of sodium alginate, selenium-enriched yeast, selenomethionine and selenocysteine, and an additive II composed of milkvetch, kudzuvine root, gynostemma pentaphyllum, shrimp shell meal, ramie leaf powder and lotus leaf powder; after one month of a lying-in period of the laying hens, the additive I and the additive II are alternately added into a feed, so that the content of selenium in the eggs is maintained to be 0.35-1.65mg / kg; compared with common eggs, the eggs laid by the laying hens fed with the feed additive have the advantages that the content of cholesterol is reduced by more than 35%, the content of protein is increased by more than 25%, the content of the selenium, zinc, iron and calcium is greatly increased, functions of the laying hens are not damaged, the adverse effect on the laying hens is not generated, and the original taste of the chicken is also maintained.

The purpose of the present invention is to provide a method for producing selenoneine that allows production of selenoneine at higher yields as compared with a conventional technology, and, therefore, enables selenoneine production on an industrial scale. This purpose can be achieved by a method for producing selenoneine, comprising the step of applying histidine and a selenium compound to a transformant that has a gene encoding an enzyme of (1) below introduced therein and that can overexpress the introduced gene, to obtain selenoneine.(1) An enzyme that catalyzes a reaction in which hercynylselenocysteine is produced from histidine and selenocysteine in the presence of S-adenosylmethionine and iron (II).

The invention provides a preparation method of L-Se-methylselenocysteine. The preparation method comprises the following steps: A) carrying out a nucleophilic substitution reaction on methyl hydroselenide sodium salt with N-acetyl-3-chloro-L-serine methyl ester to obtain N-acetyl-3-selenium methyl-L-selenocysteine methyl ester; and B) hydrolyzing the N-acetyl-3-selenium methyl-L-selenocysteine methyl ester obtained in the step A) in a hydrochloric acid solution to obtain the L-Se-methylselenocysteine. According to the invention, the N-acetyl-3-chloro-L-serine methyl ester is used as a chiral source reagent, and the obtained product is a single optically active substance, so that complex and tedious splitting process can be overcome. The process has the advantages that raw materials are easily available and cheap, operation is convenient, reaction conditions are mild, the product is single, separation is easy, the yield is high, and the method is suitable for industrial production.

The invention discloses a brewing method of organic selenium healthy wine, and belongs to the technical field of healthy wine brewing. The brewing method comprises the steps of preparing distilled wine and liquid wine stillage by selenium-rich rice, adding wall-broken enzyme to the liquid wine stillage to release a selenium element (selenium protein) which is utilized by microorganisms and stored in a cell again in a fermentation process, adding protease to hydrolyze the released selenium protein into selenomethionine, selenocysteine, oligopeptide and the like which are freely soluble in Baijiu, and finally performing mixing and squeezing on the selenium-rich wine stillage and the distilled wine to improve the selenium content in the Baijiu. The total selenium content of a prepared wine body is 0.025mg / kg; the organic selenium content is 0.014mg / kg; and a requirement of an industrial standard is met.

Recombinant bacterial cells are provided that comprise a stable non-canonical amino acid translation pathway. In some aspects, the bacteria comprise nucleic acids encoding a non-canonical amino acid translation pathway (e.g., a tRNA for incorporation of a non-canonical amino acid, such selenocysteine); a marker polypeptide that includes the non-canonical amino acid. Recombinant tRNA and selection marker coding sequences are likewise provided.

An antioxidant selenium polypeptide derived from pennsylvania bittercress herb is characterized in that the amino acid sequence is Gly-Arg-Val-Gly-Ser-Ser-Ser-Ser-SeCys, wherein SeCys is selenocysteine. A method for preparing the antioxidant selenium polypeptide derived from pennsylvania bittercress herb includes the steps of powdering, enzymolysis, ultrafiltration separation, ion exchange chromatography separation and RP-HPLC separation. The idea and method of extraction and application of conventional antioxidants and selenium supplements are changed, and the side effects possibly caused byartificially synthesized antioxidants are eliminated. The extracted antioxidant selenium polypeptide of pennsylvania bittercress herb is a natural antioxidant and a natural selenium supplement, and can replace a traditional synthesized antioxidant and has the effect of selenium supplement at the same time.

The invention relates to an organic selenium-rich health feed for feeding a selenium-rich purple crab and a preparation method of the organic selenium-rich health feed. The organic selenium-rich health feed consists of high-selenium plant meal or high-selenium cereal meal, a high-anthocyanin biological material and general selenium-rich purple crab feed ingredients. In the organic selenium-rich health feed, the total selenium content is 300mcg / kg to 5000mcg / kg, wherein the percentage of selenomethionine in total selenium is 60% to 80%, and the percentage of selenocysteine in the total selenium is 1% to 10%. The feed can be used for increasing the content of selenium in meat of the selenium-rich purple crab as well as the immunity, the survival rate and the growth speed of the selenium-rich purple crab, can effectively prevent and treat diseases of the selenium-rich purple crab and can improve the breeding environment. The selenium-rich purple crab fed by using the feed is rich in selenium and obviously better than common selenium-rich purple crabs in immunity, survival rate and growth speed.

A novel selenocysteine-containing protein is provided which is a substantial entity having an enzymatic activity, typically an activity to reduce peroxide phospholipids. There are provided a gene consisting of a coding sequence for a protein not containing selenocysteine in which the codon for selenocysteine is introduced at a desired position and a selenocysteine insertion sequence resided at the 3′-end of said coding sequence and a protein expressed therefrom, typically a selenocysteine-containing protein having an activity to reduce peroxide phospholipids which is prepared by introducing selenocysteine into albumin and as well as a gene encoding said protein. A novel anti-oxidative substance is provided which can be applied for prevention of worsening, prophylaxis or treatment of various diseases associated with oxidative stress.