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Detection method of selenium form in aquatic product

A detection method and technology for aquatic products, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of no determination of the form of selenium in aquatic products, and achieve the results of accurate and reliable detection data, accurate scientific evaluation, and reasonable selection of experimental conditions

Inactive Publication Date: 2013-11-20
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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Problems solved by technology

This detection method aims to provide technical support and method basis for revealing the occurrence form of selenium in aquatic products and scientifically formulating the limit of toxic inorganic selenium in aquatic products. At present, there is no detection method for the determination of selenium form in aquatic products in the international ISO and CAC standards

Method used

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  • Detection method of selenium form in aquatic product
  • Detection method of selenium form in aquatic product
  • Detection method of selenium form in aquatic product

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Experimental program
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Effect test

Embodiment 1

[0052] Embodiment 1: the detection of selenium form in the scallop:

[0053] 1. Selected instruments and equipment: HPLC-HGAFS combined system:

[0054] (1) For the HPLC part, use LC-10ATVP high-pressure liquid phase pump; 7725i six-port sampling valve (Rheodyne, Cotati, CA, USA) equipped with 100μL quantitative loop; use Hamilton PRP-X100, 250mm×4.1mm i.d, 10μm anion chromatographic column, equipped with a guard column of the same material packing 25mm×2.3mmi.d, 12-20μm;

[0055] (2) HGAFS-9130 atomic fluorescence photometer, equipped with excitation light source, high-performance selenium hollow cathode lamp, speciation analysis and measurement software (Beijing Jitian Instrument Co., Ltd.);

[0056] (3) Cooling circulating water (model SHA-C);

[0057] (4) Super constant temperature mixer (model TMS-200);

[0058] (5) Sand core filter (model SH / T0093);

[0059] (6) Circulating vacuum pump (model SHZ-D);

[0060] (7) Centrifuge (model LD5-10);

[0061] (8) CNC ultrason...

Embodiment 2

[0102] Embodiment 2: to the mensuration of selenium form in sea cucumber:

[0103] Accurately weigh 0.500g of dry sample that has been crushed through a 40-mesh sieve (can be determined according to the sample content) and put it into a 25mL graduated test tube with stopper, add 10mL of water and mix well, or weigh 2.000g of fresh sample (need to be mashed and homogenized) g-4.000g (can be determined according to the sample content) in a 25mL stoppered graduated test tube, add 10mL water and mix well, shake at 70°C for 1 hour with a super constant temperature mixer to fully extract the sample, take it out and cool. Centrifuge at 4000r / min for 15min, take out an appropriate amount of supernatant and filter it with a 0.45 micron water-based microporous membrane, and measure the value of the form of selenium on the machine. Other conditions are the same as those of scallops. The measurement results of sea ginseng standard recovery are shown in Table 3, and its selenium occurrence...

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Abstract

The invention relates to a detection method of selenium form in an aquatic product, and belongs to the technical field of aquatic product detection. The selenium form in the aquatic product is extracted by oscillating with a super constant-temperature mixer and measured by a high performance liquid chromatography-hydride generation atomic fluorescence spectroscopy combined morphological analysis technology, the extracting solution is separated by an anion chromatographic column, different forms of selenium are eluted successively by a moving phase, hydrogenation is carried out on the elution solution through a potassium borohydride reducing agent and hydrochloric acid to generate a hydride which enters an atomizer and is used in combination with atomic fluorescence for analysis and measurement, so that a more complete biological property of the selenium is provided. The experiment condition is reasonable, the detection data are accurate and reliable, the sample recovery rate is 88.79-94.88% and the degree of precision is less than 5%. The method is high in sensitivity, low in interference and simple in sample pretreatment, can be used for accurately measuring inorganic selenium, selenite and selenate as well as organic selenium, selenocysteine and selenomethionine and detecting selenium form in aquatic product.

Description

technical field [0001] The invention belongs to the technical field of aquatic product detection, and in particular relates to a detection method for determining the form of selenium in aquatic products by using high performance liquid chromatography-hydride generation atomic fluorescence spectrometry (HPLC-HGAFS) form analysis technology. Background technique [0002] At present, one of the frontiers of elemental analytical chemistry is the qualitative and quantitative analysis of different forms of elements, not just the determination of the total amount of elements, mainly because different forms of the same element have completely different chemical and toxicological properties . Selenium is an essential trace element for the human body, and its form is often divided into inorganic selenium and organic selenium. Inorganic selenium includes elemental selenium (Se), selenium compounds (Se 2- ), selenate (SeO 4 2- ) and selenite (SeO 3 2- ); organic selenium includes ...

Claims

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Application Information

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IPC IPC(8): G01N30/88
Inventor 尚德荣赵艳芳翟毓秀宁劲松丁海燕盛晓风
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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