98 results about "Penicillamine" patented technology

The present invention provides prodrugs comprising a polymer conjugated to a metal chelator via a disulfide bond. For example, D-penicillamine may be conjugated to a polymer (e.g., gelatin, chitosan, polyglutamic acid) via a linker, such as SPDP. Thus, the cellular delivery and pharmacokinetics of D-penicillamine can be substantially improved. Methods for the treatment of cancer using compositions of the present invention are also disclosed.

The present invention provides methods of making α4β7 peptide monmer and dimer antagonists. Methods of the present invention include solid phase and solution phase methods, as well as synthesis via condensation of smaller peptide fragments. Methods of the present invention further include methods directed to the synthesis of peptides comprising one or more penicillamine residues.

The invention discloses a valnemulin synthesis method which comprises the following steps of: 1. pleuromutilin sulphonic acid ester synthesis: using pleuromutilin as raw materials to react with sulfonic acid chloride under the alkaline condition for converting the pleuromutilin into pleuromutilin sulphonic acid ester; 2. sulpho-pleuromutilin synthesis: using penicillamine to substitute sulphonic acid ester in the product in the step 1 under the alkaline condition to obtain sulpho-pleuromutilin; 3. amino-protected D-R-valine synthesis; and 4. valnemulin synthesis. The mainly used raw materials of the valnemulin synthesis method are fermentation products of the pleuromutilin and cysteine which can be easily obtained in the market and have low cost, and in addition, the valnemulin synthesis method has simple process operation, has no danger, has the characteristics of low cost, high yield and the like, is applicable to enlargement and industrial production and has industrialization value.

The invention discloses a nickel-cobalt sulpho-spinel for super capacitor and a preparation method of the nickel-cobalt sulpho-spinel and aims to further improve the pseudocapacitance feature of nickel-cobalt metal compound composite material and increase the specific discharge capacity and cycling stability of the nickel-cobalt metal compound composite material. The preparation method includes: nickel salt, cobalt salt and penicillamine are used as raw materials, a solvent-thermal method is used, precursor nickel salt, cobalt salt and penicillamine proportion, solvent varieties, solvent-thermal temperature / time, pH values and the varieties of used inorganic acids are controlled, and subsequent procedures of centrifuging / washing / drying / roasting and the like are performed to obtain the nickel-cobalt sulpho-spinel nano material. Tests show that when the nickel-cobalt sulpho-spinel nano material is used as electrode material to produce a super capacitor, high specific discharge capacity and cycling stability are achieved. In addition, the preparation method is low in reaction temperature, controllable in reaction, capable of satisfying the requirements of industrial production, suitable for large-batch production and promising in development prospect.

Articles of manufacturing, methods, devices and compositions for preventing or reducing tobacco-associated damage in a subject, and which utilize a metal ion chelating agent, a copper chelating agent, a penicillamine and / or a structural analog of penicillamine, with and without an additional antioxidant, are disclosed.

In various aspects, the invention provides methods for treating ocular inflammation using copper chelating compounds, such as compounds other than D-penicillamine. In some embodiments, such compounds may be polyamines, such as triethylenetetramine or tetraethylenepentamine. For example, the present invention provides methods for treating inflammation secondary to ocular laser therapy.

The invention discloses a preparation method of antibacterial polyester fabrics and belongs to the technical field of textile materials. The preparation method comprises the following steps: after sequentially carrying out continuous modification treatment on polyester fabric original samples including clothing, tablecloth and curtains by virtue of a Na2CO3 solution, a mixed solution of alizarin red and sodium cyclamate, a mixed solution of aniline, hydrochloride and ammonium persulfate, a mixed solution of DL-mercaptosuccinic acid and D-penicillamine and a nano-silver solution, putting the treated polyester fabric original samples into a silver tetrafluoroborate aqueous solution, carrying out reaction at room temperature for 30 minutes, washing the product by a great deal of water, and drying at the temperature of 40 DEG C to obtain the antibacterial polyester fabrics. The antibacterial polyester fabrics prepared by the preparation method have the broad-spectrum antibacterial property, are good in antibacterial effect particularly on staphylococcus aureus, bacillus subtilis, Escherichia coli and pseudomonas aeruginosa, are strong in persistence and have the single-strain inhibitory rate reaching 95-99.7 percent after being washed for 30 times.

The invention discloses a preparation method for a chiral CdSe quantum dot. The preparation method comprises the following steps: (1) preparing a sodium hydrogen selenide aqueous solution, and carrying out preservation at 4 DEG C or below; (2) dissolving cadmium chloride and a ligand in water, adjusting a pH value of an obtained mixture to 7 to 12 so as to obtain a precursor solution, and subjecting the precursor solution to stirring in an ice bath under the protection of inert gas, wherein the ligand is a combination of two or more selected from the group consisting of penicillamine, cysteine, N-acetyl-cysteine, glutathione or N-isobutyryl-cysteine; and (3) injecting the sodium hydrogen selenide aqueous solution prepared in the step (1) into the precursor solution obtained in the step (2), carrying out heating to 70 to 220 DEG C for 10 to 120 min, and carrying out cooling to a room temperature so as to obtain a chiral CdSe quantum dot solution. The quantum dot provided by the invention can be extensively applied to the fields of enantiomer recognition and separation, nanometer equipment, chiral sensors, asymmetric catalysis, etc.

The invention belongs to the field of chemical industry and relates to a preparation method of functionalized graphene quantum dots producing ultrasensitive fluorescence response to Fe<3+>. Citric acid and a certain mole ratio of penicillamine are put in a beaker and are dissolved in water to form a mixed acid solution; the obtained mixed acid solution is transferred to a high-pressure reaction kettle, heating reaction and freeze drying are performed to obtain the functionalized graphene quantum dots; the functionalized graphene quantum dots are dissolved in water to obtain a graphene water solution; a buffer solution is mixed with a Fe<3+>-containing solution and reacts with the prepared functionalized graphene quantum dot water solution for a certain time, then the fluorescence intensity is determined on a fluorescence spectrophotometer, and the Fe<3+> content in a sample is calculated on the basis. The citric acid and the penicillamine are used as a mixed carbon source to prepare the functionalized graphene quantum dots through one-step hydrothermal reaction, remarkable fluorescence quenching is produced by utilizing specific combination between the Fe<3+> and the quantum dots, and high sensitivity and high-selectivity determination conducted on Fe<3+> in iron supplementing health care products is achieved.

The invention discloses a preparation method of a chiral Co-MOF / CoSR / PU core-shell composite material and application of the chiral Co-MOF / CoSR / PU core-shell composite material in efficient electrochemical recognition of penicillamine enantiomers, and belongs to the technical fields of nano-catalysis, chiral sensing and the like. The preparation method comprises the following main steps: synthesizing Co-MOF at room temperature, adding L-cysteine for chiral modification, and polymerizing with isophorone diisocyanate to obtain the chiral Co-MOF / CoSR / PU core-shell composite material. The composite material is low in raw material cost, simple in preparation process and low in reaction energy consumption. The prepared chiral sensor is used for sensitively detecting the content of L-penicillamine and D-penicillamine enantiomers, the method is simple and easy to operate, and the chiral detection effect is remarkable.

The present invention relates to methods for the chemo-selective pegylation of the cysteine residue having unoxidized sulfhydryl side-chain and free α-amino group in proteins, peptides and other molecules. Similar methods are provided for the chemo-selective pegylation of the homocysteine, selenocysteine, penicillamine, and N-methyl-cysteine residues.

The invention discloses a preparation method of a double-chirality beta-CD @ Cu-MOF nano composite sensor and application of the sensor as an electrochemical sensing tyrosine and penicillamine isomerat the same time, and belongs to the technical field of nano composite material technology, electro-catalysis technology and isomer recognition technology. The preparation method comprises the following main steps of: mixing a copper nitrate solution and a ligand solution, and standing at room temperature to prepare Cu-MOF nano fibers; and blending the Cu-MOF nano fibers and beta-cyclodextrin beta-CD, and dropwise coating a glassy carbon electrode GCE with the mixture to prepare the double-chirality beta-CD @ Cu-MOF / GCE nano composite sensor. The sensor is low in raw material cost and low inreaction energy consumption, and the preparation method is simple and efficient. The sensor is applied to electrochemical sensing of tyrosine and penicillamine isomers at the same time, has the advantages of high sensitivity, simple equipment, high electrochemical stability and the like, and has a good industrial prospect.

The invention discloses a preparation method of a chiral MOF supermolecule composite material and an application of such material in efficient electrochemical identification of a penicillamine enantiomer and belongs to the technical field of nanomaterials, electrochemical chiral recognition and metal organic frame materials. The method mainly comprises the following steps: synthesizing a chiral L-glutamic acid bolaamphiphile L-HDGA by using hexadecyl diacid and diethyl glutamate, and then, preparing a spirally-arranged L-HDGA template with ethanol and water as solvents; and through a self-assembly method, preparing the composite material with ZIF 67 nanoparticles loaded on the surface of the L HDGA spiral structure. The application of the material in efficient electrochemical identification of the penicillamine enantiomer has the advantages of simple process, fast response and wide electrochemical identification; and the material has an application prospect.

The invention discloses a preparation method of a gold nanocluster emitting red fluorescence and a detection method for penicillamine and also discloses a sensitive detection method for penicillaminein actual water samples and urine. The method for preparing the gold cluster is simple, the reaction condition is mild, the obtained gold cluster has good light stability and water solubility and highquantum yield. The gold cluster is combined with copper ions to build a fluorescence-quenching sensing system which can be used for fluorescence-enhanced detection of the penicillamine; the detectionmethod for the penicillamine is low in detection limit, good in selectivity and strong in interference resistance and can be used for detecting the penicillamine in the complex actual samples; when the samples contain the penicillamine, the existence of the penicillamine in the actual samples can be judged only by using a simple handheld ultraviolet lamp to observe the change of the color of thesample solution by the naked eyes.

The invention discloses a preparation method of an Ag / Cu alloy nano-cluster and application of the Ag / Cu alloy nano-cluster in Ag < + > detection. The preparation method comprises the following steps:sequentially adding a D-penicillamine solution, an AgNO3 solution and a Cu (NO3) 2 solution into a container filled with ultrapure water, and uniformly mixing to obtain a mixture; and stirring the mixture with a magnetic stirrer, and irradiating with an ultraviolet lamp for 4-10 hours to obtain the DPA-Ag / Cu alloy nano-cluster solution capable of emitting yellow fluorescence. The preparation method is low in cost, easy and convenient to operate, free of toxic reagents and more environmentally friendly. The prepared DPA-coated Ag / Cu NCs are used for constructing a fluorescence sensor for detecting Ag < + > in a water environment. The fluorescence quenching value and the Ag < + > concentration of the fluorescent probe have a good linear relation within the range of 0.01-800 [mu] M, the detection selectivity and sensitivity are high, and the fluorescent probe has an important practical application value.

Treatment of mitochondrial related conditions in mammals with antagonists or chelating agents of copper (II), preferably tetramines or penicillamines. These agents affect TGF-beta, Smad 4, collagen IV, cytochrome C oxidase and erectile dysfunction.

The present invention provides methods of making α4β7 peptide monomer and dimer antagonists. Methods of the present invention include solid phase and solution phase methods, as well as synthesis via condensation of smaller peptide fragments. Methods of the present invention further include methods directed to the synthesis of peptides comprising one or more penicillamine residues.

A composition of variant biocatalysts, specifically variants of D-amino acid oxidases, with improved biocatalytic activity towards D-amino acid substrates such as, but not limited to, D-tert-leucine, D-norvaline, D-2-aminobutyrate, D-alanine, D-isoleucine, D-valine, D-methionine, D-hydroxyadamantlyglycine, D-penicillamine, or D-norleucine is disclosed. Further disclosed is a method of preparing enantioselective amino acids using variant D-amino acid oxidases of the present invention.

The invention discloses a method for measuring the amount of residual penicillin in milk. Standard working anhydrous alcohol solution with penicillin sodium is prepared, the pH value is regulated by dilute HCl to 2 plus or minus 0.5 or 4 plus or minus 0.5, the standard working anhydrous alcohol solution is bathed in boiling water, so that degradation products, i.e. penicillamine and penicillenic acid, are obtained, then the two degradation products respectively react with benzoquinone substance, so that charge-transfer complexes are obtained, and the working curves of different charge-transfer complexes are calculated. During measurement, the absorbance of the charge-transfer complex of milk sample is calculated by the same way, and is substituted into the working curve of the charge-transfer complex to work out the amount of the residual penicillin in the milk. The invention takes the lead in directly using the ultraviolet or fluorescent spectrophotometry to measure the amount of the residual penicillin in the milk. The method has the advantages of quick measurement, wide measurement range, high sensitivity, high accuracy and good selectivity, an expensive antibiotic detection instrument does not need to be purchased, the operation method is simple, and the method can reduce the production cost and the operation risk for diary processing enterprises, and therefore having a good application prospect.

The invention discloses a cyclodextrin-base nitric oxide donor and a method for preparing the same. The cyclodextrin-base nitric oxide donor is shown as a formula (I). The method includes dissolving reaction products of amination cyclodextrin and N-acetyl penicillamine thiolactone in organic solvent; adding nitroso tert-butyl ester into the organic solvents; carrying out complete reaction under certain conditions and then precipitating reaction products by the aid of poor solvents to obtain the cyclodextrin-base nitric oxide donor. The cyclodextrin-base nitric oxide donor and the method have the advantages that the cyclodextrin-base nitric oxide donor prepared by the aid of the method can be widely applied to medicine development, for example, the cyclodextrin-base nitric oxide donor can be used for preparing subcutaneous, intramuscular or intravenous injection, the subcutaneous, intramuscular or intravenous injection can be used for treating cardiovascular diseases, tumor and the like, accordingly, excellent treatment effects can be realized, and the cyclodextrin-base nitric oxide donor and the method have popularization and application value.

The invention relates to a method for performing in-situ quantitative detection on interaction of gold nanoparticle chiral surface adsorption protein and a cytomembrane associated receptor based on a quartz crystal chip. The method comprises the following steps: (1) pretreating the quartz crystal chip by 2-mercaptopropionic acid and 2-mercaptoethanol; (2) putting the treated chip into a QCM-D instrument, introducing EDC / NHS and activating the carboxyl on the surface of the chip; (3) introducing gold nanoparticles with the surface modified by penicillamine micromolecules and combining an amido bond and the chip; (4) introducing ligandin and enabling the ligandin to be adsorbed on the chiral surface of the nanogold; (5) introducing cell derived lipidosome with the surface containing the associated receptor. The interaction of the ligandin and the associated receptor is quantified through the intensity of a frequency signal. The method is simple to operate and high in practicability, and has important significance in research on the structure and the function of protein crown formed on the surfaces of the nanoparticles.

The present disclosure relates to a method of maintaining luminal patency of a blood vessel following vessel injury, the method including administering a composition comprising at least one lysyl oxidase inhibitor and D-penicillamine to a subject in need thereof Compositions to support the methods are also provided.

The invention belongs to the technical field of fresh keeping of fruits, and particularly relates to a fresh keeping agent and method for preventing picked wax apple fruits from becoming flocculent and soft. The fresh keeping agent contains the following components: S-nitroso-N-acetyl-DL-penicillamine of 20-350 mu mol / L, rootone of 0.5-30 mu mol / L, gibberellin of 30-300 mu mol / L, and N-( 2-chloro-pyridyl)-N'-phenylurea of 10-150 mu mol / L. The method for fresh keeping of wax apple fruits with the fresh keeping agent comprises the following steps of putting the wax apple fruits in a container, pouring the fresh keeping agent, and enabling the wax apple fruits to be immersed in a fresh keeping agent solution for 3-20 minutes. Through the adoption of the fresh keeping agent, the wax apple fruits can be effectively prevented from becoming flocculent and soft, fruit rot is avoided, the hardness of the fruits is maintained, the fresh-eating period of the wax apples is prolonged, and the commodity value of the wax apple fruits is increased.

The invention discloses a synthesis method of chiral copper sulfide superparticles, and belongs to the technical field of nanometer materials and self-assembly science. Penicillamine-stabilized chiralcuprous sulfide nanoclusters and tobacco mosaic virus capsid protein are subjected to self-assembly under the action of light to obtain cake-shaped chiral cuprous sulfide superparticles. The invention provides a light-operated preparation method of chiral copper sulphide superparticles, and the preparation method has important significance for developing more chiral materials and understanding the interaction between the chiral materials.

Provided is a cyclic peptide, which is represented by Formula (I) or Formula (I′) and has excellent antibody binding properties and improved chemical resistance, an affinity chromatography support, a labeled antibody, an antibody drug conjugate, and a pharmaceutical preparation.RN-Xg-[Xi-Xa-Xm-X2-X3-Xn-Xb-Xj]k-Xh-RC  (I)In Formula (I), Xa and Xb each independently represent an amino acid residue derived from an amino acid, other than L-cysteine and D-cysteine, having a thiol group on a side chain and are bonded to each other through a disulfide bond, or, one of Xa and Xb represents an amino acid residue derived from an amino acid, other than L-cysteine and D-cysteine, having a thiol group on a side chain and the other represents an amino acid residue derived from an amino acid having a haloacetyl group on a side chain, and Xa and Xb are bonded to each other through a thioether bond.RN-Xg-[Xi-Xa-Xm-X1-X2-X3-Xn-Xb-Xj]k-Xh-RC  (I′)In Formula (I′), one of Xa and Xb represents an amino acid residue derived from L-cysteine or D-cysteine and the other represents an amino acid residue derived from an amino acid having a haloacetyl group on a side chain, and Xa and Xb are bonded to each other through a thioether bond, or, one of Xa and Xb represents an amino acid residue derived from L-penicillamine or D-penicillamine and the other represents an amino acid residue derived from an amino acid having a haloacetyl group on a side chain, and Xa and Xb are bonded to each other through a thioether bond.

The invention provides a compound of formula I: wherein R1-R3 have any of the values described in the specification; or a salt thereof. The compounds are useful as aldehyde sequestration agents (e.g., in the treatment of alcohol-related diseases). The invention also provides compositions comprising such compounds as well as therapeutic methods comprising their administration.

The invention provides a penicillamine-based polypeptide disulfide bond synthesis method. The method adopts a route 1 or 2, wherein the route 1 comprises the following steps: dissolving iodine in N,N-dimethylformamide, adding the mixture into resin A with synthesized related sequences, performing a nitrogen blowing reaction, cutting off polypeptide by using shearing liquid, and separating and purifying the mixture with high performance liquid chromatography to obtain a target product; and the route 2 comprises the following steps: cutting off the polypeptide with the synthesized related sequence from the resin A by utilizing the shearing liquid, then separating out non-oxidized and ring-closed polypeptide by using the high performance liquid chromatography, dissolving the polypeptide in a phosphate buffer solution with the pH value of 7.4-8.0, adding an oxidant into the mixture for oxidation, and carrying out separation and purification by using the high performance liquid chromatography to obtain the target product. The invention provides the novel stable disulfide bond designing and synthesizing strategy, and the disulfide bond serving as the bullet of an effective covalent reaction is applied to the field of chemical biology.

The present invention relates to water-soluble binuclear cationic nitrosyl iron complexes with natural aliphatic thiolyls of general formula [Fe2(SR)2(NO)4]SO4,wherein R is sulfur-containing aliphatic ligands of natural origin, preferably, cystamine or penicillamine, to a method for the preparation thereof, to use thereof as an anticancer agent, to an apoptosis inducer, to a pharmaceutical composition comprising said complexes in an effective amount, and to a kit useful for the treatment of cancer diseases.