38 results about "Valnemulin" patented technology

The invention discloses a preparation method for a valnemulin liposome. The method comprises the following steps: 1) preparation of a NaHCO3 solution; 2) preparation of a citric acid buffer solution; 3) preparation of a blank liposome: a step of dissolving phosphatide and cholesterol in absolute ethyl alcohol in a water bath, allowing ethanol to volatilize and a film to be formed, then adding the citric acid buffer solution, carrying out complete hydration and filtering an obtained liposome solution with a millipore filter to realize whole grain filtering; 4) preparation of valnemulin fluid by dissolving raw valnemulin powder in ethanol; 5) active loading: a step of successively adding the valnemulin fluid and the NaHCO3 solution into the blank liposome and carrying out uniform mixing so as to obtain a crude liposome product; and 6) low temperature high speed centrifugation of the crude product obtained in step 5) so as to prepare the valnemulin liposome. According to the invention, through preparation of the blank liposome and active loading, the valnemulin liposome with a high entrapment rate and good clinical effects is obtained; the method is simple and easily practicable, and solubility of valnemulin in water and effective acting time, stability and targeting of valnemulin are improved.

The invention relates to a drug formula for preventing and treating the chronic PRDC (porcine respiratory disease complex). The drug formula comprises raw materials, namely, valnemulin, enrofloxacin and traditional Chinese medicine powder, wherein the traditional Chinese medicine powder comprises raw materials, namely, codonopsis pilosula, liquorice roots, poria cocos, largehead atractylodes rhizomes, lily bulbs, dwarf lilyturf tubers, purified pinellia tubers, tatarian aster roots, ephedra herbs, thinleaf milkwort roots, common anemarrhena rhizomes, coastal glehnia roots, dried tangerine peels and pumice, the Chinese herbal medicines are distilled with water vapor, an extract is obtained, the extract and fine pumice powder produced through milling by an efficient ball mill are evenly mixed, then dried at the low temperature and packaged in a vacuum manner, and the traditional Chinese medicine powder is prepared. The formula is significant in curative effect on the chronic PRDC, less in traditional Chinese medicine addition amount and good in palatability, takes effect quickly and is high in cure rate according to clinical usage.

The invention discloses a valnemulin artificial antigen and a preparation method and application thereof. The valnemulin artificial antigen is a compound as shown in a formula I, wherein n denotes a natural number from 1 to 25; and K denotes carrier protein. When the artificial antigen is used to immunize a New Zealand white rabbit, an antibody with specific reaction to valnemulin can be obtained. According to ELISA experiments, the titer of the obtained antiserum reaches 1:102400. The invention provides a wide application and development space for the development of detection reagent used inthe enzyme-linked immunoassay method of the valnemulin and the preparation of the enzyme-linked immunoassay reagent kit of the valnemulin. When the antibody obtained by using the artificial antigen is used for the detection of the valnemulin, the operation is simple and convenient, the specificity is good and the accuracy is high. The antibody can be used for the rapid and high-efficiency screening of the valnemulin in animal-based foods and a technical foundation is laid for the rapid detection of the valnemulin. The formula I is as shown is the accompanying drawing.

The invention relates to a novel crystal form of valnemulin hydrogen tartrate and a preparation method thereof. The method comprises the steps of dissolving valnemulin hydrogen tartrate with the purity being over 95% into a mixed solvent of an ester solvent and N,N-dimethylformamide, adding an elution agent after complete dissolution so as to carry out elution crystallization, then, cooling a solution to the temperature of 5-15 DEG C, and washing, filtrating and drying crystal slurry, thereby obtaining a valnemulin hydrogen tartrate product. An X-ray powder diffraction atlas of the crystal has characteristic peaks when a diffraction angle 2[theta] is equal to 2.5, 4.8, 7.4, 7.5, 10.1, 11.2, 11.7, 13.8 or 17.1 degrees. According to the novel crystal form, during crystallization, the yield reaches over 90%, the product purity reaches over 98%, the product is high in crystallinity, good in stability, difficult in moisture absorption and high in solubility, the water solubility at normal temperature reaches 6.5g / 100mL and is increased by about 30% compared with that of the currently marketed products, and the water stability is good, so that the product can be conveniently prepared into a premix.