Valnemulin artificial antigen and preparation method and application thereof

Abstract

The invention discloses a valnemulin artificial antigen and a preparation method and application thereof. The valnemulin artificial antigen is a compound as shown in a formula I, wherein n denotes a natural number from 1 to 25; and K denotes carrier protein. When the artificial antigen is used to immunize a New Zealand white rabbit, an antibody with specific reaction to valnemulin can be obtained. According to ELISA experiments, the titer of the obtained antiserum reaches 1:102400. The invention provides a wide application and development space for the development of detection reagent used in the enzyme-linked immunoassay method of the valnemulin and the preparation of the enzyme-linked immunoassay reagent kit of the valnemulin. When the antibody obtained by using the artificial antigen is used for the detection of the valnemulin, the operation is simple and convenient, the specificity is good and the accuracy is high. The antibody can be used for the rapid and high-efficiency screening of the valnemulin in animal-based foods and a technical foundation is laid for the rapid detection of the valnemulin. The formula I is as shown is the accompanying drawing.

Description

technical field The invention relates to a warnemulin artificial antigen and its preparation method and application. Background technique Valnemulin is a new generation of pleuromutilin (pleuromu.tilin) ​​semi-synthetic antibiotics. It belongs to diterpenes and is a similar drug to tiamulin. The raw material drug of warnemulin that is now on the market is warnemulin hydrochloride, and its chemical name is {[2~((R)-2-amino-3-methylbutyrylamino)-1,1-dimethylethyl Group] mercapto}acetic acid (3as, 4R, 5S, 6S, 8R, 9R, 9aR, IOR)-octahydro-5,8-dihydroxy-4,6,9,10-tetramethyl-6-vinyl- 3a, 9-propane-3aH-cyclopentacyclooctene-1(4H)-ketone-8-ester hydrochloride, molecular formula C3lH53CINz 05S, relative molecular weight 601.30, chemical structure formula see formula (II). Warnemulin hydrochloride is white or light yellow non-crystalline powder with hygroscopicity; easily soluble in water and absolute ethanol, almost insoluble in tert-butyl methyl ether; specific rotation is +15.5°~+...

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Problems solved by technology

Although studies have reported that warnemulin is less prone to drug resistance compared with other drugs, its clinical application time is not long, and it cannot prove its results after long-term use

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