Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Leaf-shaped metal organic framework nano artificial enzyme hybrid and preparation method of tandem catalytic fluorescent probe from enzyme hybrid

A metal-organic framework, fluorescent probe technology, applied in fluorescence/phosphorescence, material analysis by optical means, measurement device, etc., can solve the problem that there is no tandem catalytic uric acid fluorescent probe.

Pending Publication Date: 2021-03-26
QINGDAO UNIV
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are no domestic and foreign literature and patent reports on UOx@MOF(AuNC) hybrids and their tandem catalytic uric acid fluorescent probes.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Leaf-shaped metal organic framework nano artificial enzyme hybrid and preparation method of tandem catalytic fluorescent probe from enzyme hybrid
  • Leaf-shaped metal organic framework nano artificial enzyme hybrid and preparation method of tandem catalytic fluorescent probe from enzyme hybrid
  • Leaf-shaped metal organic framework nano artificial enzyme hybrid and preparation method of tandem catalytic fluorescent probe from enzyme hybrid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] The preparation method and schematic diagram of the leaf-shaped metal-organic framework nano-artificial enzyme hybrid and its tandem catalytic fluorescent probe involved in this example are as follows figure 1 As shown, the specific preparation steps are as follows:

[0021] Weigh 0.15 mmol of zinc nitrate and 1 mmol of 2-methylimidazole to prepare an aqueous solution, and fully mix the aqueous solutions of zinc nitrate and 2-methylimidazole under magnetic stirring at room temperature to form a homogeneous solution of 10 ml; add 1 ml of commercial The gold nanocage dispersion liquid was stirred and reacted for 10 minutes; then 10 mg of urate oxidase was added, and the reaction was continued to stir for 10 minutes; after the reaction, the product solution was left to stand at 4 degrees Celsius for 12 hours, and then centrifuged at 5000 rpm for 15 minutes , repeated washing and freeze-drying, and finally the leaf-shaped metal-organic framework nanoartificial enzyme hybrid...

Embodiment 2

[0023] The preparation method and schematic diagram of the leaf-shaped metal-organic framework nano-artificial enzyme hybrid and its tandem catalytic fluorescent probe involved in this example are as follows figure 1 As shown, the specific preparation steps are as follows:

[0024] Weigh 0.2 millimoles of zinc nitrate and 1.5 millimoles of 2-methylimidazole to prepare an aqueous solution, and fully mix the aqueous solutions of zinc nitrate and 2-methylimidazole under magnetic stirring at room temperature to form 15 ml of a homogeneous solution; add 1.5 milliliters of commercial The gold nanocage dispersion liquid was stirred and reacted for 15 minutes; then 15 mg of urate oxidase was added, and the reaction was continued to stir for 15 minutes; after the reaction, the product solution was left to stand at 4 degrees Celsius for 12 hours, and then centrifuged at 5000 rpm for 20 minutes , repeated washing and freeze-drying, and finally the leaf-shaped metal-organic framework nano...

Embodiment 3

[0026] The preparation method and schematic diagram of the leaf-shaped metal-organic framework nano-artificial enzyme hybrid and its tandem catalytic fluorescent probe involved in this example are as follows figure 1 As shown, the specific preparation steps are as follows:

[0027] Weigh 0.25 millimoles of zinc nitrate and 2 millimoles of 2-methylimidazole to prepare aqueous solutions, and fully mix the aqueous solutions of zinc nitrate and 2-methylimidazole under magnetic stirring at room temperature to form 20 ml of a homogeneous solution; add 2 ml of commercial The gold nanocage dispersion liquid was stirred and reacted for 20 minutes; then 20 mg urate oxidase was added and the reaction was continued to stir for 20 minutes; after the reaction, the product solution was left to stand at 4 degrees Celsius for 18 hours, and then centrifuged at 5000 rpm for 20 minutes , repeated washing and freeze-drying, and finally the leaf-shaped metal-organic framework nanoartificial enzyme hy...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a leaf-shaped metal organic framework nano artificial enzyme hybrid and a preparation method of a tandem catalytic fluorescent probe from the enzyme hybrid. A novel gold nanocage (AuNC) and urate oxidase (UOx) double-loaded metal organic framework compound (MOF) is prepared in a water phase by adopting a one-pot method, and is used as a leaf-shaped metal organic framework nano artificial enzyme hybrid UOx @MOF (AuNC) for catalyzing uric acid to generate hydrogen peroxide, and the hydrogen peroxide reacts with ferrous ions to generate .OH hydroxyl radicals; the .OH hydroxyl radicals cause fluorescence quenching of rhodamine B. The linear relationship between the fluorescence emission peak intensity of rhodamine B in the mixed system and the concentration of uric acidis fit, and the novel fluorescent probe based on the hybrid and the tandem catalytic reaction thereof is constructed, and is used for high-sensitivity and specific quantitative detection of uric acid.

Description

technical field [0001] The invention belongs to the technical field of preparation of nano-artificial enzyme hybrids and tandem catalytic fluorescent probes, and specifically relates to a method for preparing a tandem catalytic fluorescent probe based on leaf-shaped metal-organic framework nano-artificial enzyme hybrids. The probe can be used for highly sensitive and specific quantitative detection of uric acid in biological fluids. Background technique [0002] The chemical structure of uric acid (Uric acid, UA) is trioxypurine, which is the end product of purine metabolism. After oxidation of various purines, uric acid is produced and excreted in urine. When the body produces too much uric acid and cannot be discharged in time, it will cause the body fluids to become sour, affect the normal function of human cells, and even cause gout. All uric acid in the blood is filtered from the glomerulus, most of the uric acid is reabsorbed in the proximal convoluted tubule and the ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/6428G01N2021/6432
Inventor 桂日军孙泽君杨梦
Owner QINGDAO UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com