The invention discloses a variable region sequence of a broad-spectrum antibody resisting clothianidin and dinotefuran. The amino acid sequence of a heavy chain variable region coding gene is shown asSEQ ID NO: 2. The invention also discloses a broad-spectrum recombinant complete antibody resisting clothianidin and dinotefuran. The broad-spectrum recombinant complete antibody resisting clothianidin and dinotefuran comprises a heavy chain constant region, a heavy chain variable region, a light chain constant region and a light chain variable region, and the amino acid sequence of the heavy chain variable region coding gene is shown as SEQ ID NO: 2. The sequence genes obtained by the invention are respectively connected to an expression vector containing a heavy chain constant region gene and a light chain constant region gene, and a recombinant complete antibody is obtained by adopting mammalian cell expression of a double-plasmid system, so that the recognition activity of a murine parent antibody is ensured, the broad-spectrum antibody resisting clothianidin and dinotefuran can be stably produced on a large scale, and a reliable core reagent is provided for various immunoassay methods for synchronously detecting the clothianidin and the dinotefuran.