Method for detecting Miltenberger blood group antibody

Abstract

The invention discloses a method for detecting a Miltenberger blood group antibody. The method comprises the steps of coupling nano magnetic beads with avidin to form avidin magnetic beads; coupling the avidin magnetic beads with a multipeptide antigen with a biotin-modified tail end, and closing non-coupled sites to obtain immune magnetic beads; uniformly mixing the immune magnetic beads with a detection sample, then performing incubation and washing, adding fluorescence labeled second antibody or enzyme-labeled second antibody, performing incubation and washing, and measuring a result. In the mode, the method for detecting the Miltenberger blood group antibody based on nano magnetic bead immunoassay is low in operation difficulty and is simple and convenient to operate, can be used for simultaneously detecting complete antibodies and incomplete antibodies; the detection process is short in time, and the result is easy to judge; automation is easily realized, and the detection result is accurate; in combination with a detector, semi-quantitative or quantitative detection can be realized; clinical safe, effective and scientific blood transfusion can be guaranteed.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a new method for detecting Miltenberger blood group antibodies, that is, a method for detecting Miltenberger blood group antibodies based on nano-magnetic bead immunoassay. Background technique [0002] The current routine clinical blood group identification includes the ABO and Rh blood group systems. There is also MNS blood type in red blood cells. In this system, there is a special type of subblood group system - MI subblood group system (Milternberger subsystem). Miltenberger blood group antigen is a red blood cell phenotype combined with a series of low-frequency antigens identified by human serum immune antibodies . Most of these antibodies with irregular blood group exist in the form of IgM, and a few are IgG, and the two can exist alone or at the same time. The occurrence frequency of several antigens such as Mur and Mia in this subsystem is less than 1 / 1000 in European Cau...

AI Technical Summary

Problems solved by technology

Due to the lack of relevant reagents, the antibody screening cells used by most medical institutions do not contain Miltenberger blood group antigen, resulting in missed detection of patients with this antibody

At the same time, the storage time of fresh red blood cells is short, the source of Miltenberger antigen-positive red blood cells is very limited, and the detection of Miltenberger blood group screening and its antibodies is greatly limited

At present, several main problems in the detection of Miltenberger blood group antibodies are: 1) Lack of Miltenberger blood group antigen-positive red blood cells in clinical test spectrum cells; 2) Limited source of Miltenberger blood group antigen-positive red blood cells; 3) Short storage time of red blood cells

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