The invention relates to a method for purifying a
slow virus. The method comprises the following steps: (S10) providing a
cell culture containing the
slow virus; (S20) carrying out centrifugal concentration on the
cell culture, and filtering to obtain
virus suspension; (S30) purifying the
virus suspension by virtue of
anion exchange chromatography; and (S40) purifying the
virus suspension by virtue of gel
filtration chromatography. A process for purifying the
slow virus is easy to amplify, can be used for
processing large amounts of virus liquid produced by
cell factories or bioreactors on a large scale, can meet production requirements of slow virus liquid, is stable and is good in
repeatability; furthermore, the purified slow virus liquid prepared by virtue of the method is not polluted by exogenous factors, is low in residues of cell
host protein and host
DNA, good in safety, and high in purity and
titer and can completely meet requirements of clinic
gene therapy.