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Applications of vesicle-associated membrane protein 1 in prevention and treatment of enterovirus 71 infection

A technology of enterovirus and membrane protein, applied in the field of biomedicine

Active Publication Date: 2016-05-04
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Abstract
  • Description
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AI Technical Summary

Problems solved by technology

[0007] At present, there is no report on the role of VAMP1 molecule in SH-SY5Y infection by EV71. In-depth research on this molecule can not only improve the understanding of EV71 infection and pathogenic mechanism, but also provide new ideas and ideas for the prevention and treatment of EV71 infection. target

Method used

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  • Applications of vesicle-associated membrane protein 1 in prevention and treatment of enterovirus 71 infection
  • Applications of vesicle-associated membrane protein 1 in prevention and treatment of enterovirus 71 infection
  • Applications of vesicle-associated membrane protein 1 in prevention and treatment of enterovirus 71 infection

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Embodiment 1

[0048] 1 Design and synthesis of specific siRNA sequences for each host cell molecule.

[0049] 1.1 For each target gene, search NCBI GeneBank to obtain the full sequence and mRNA sequence, use existing network resources and commonly used software to conduct biological analysis of each target gene, and select the coding region as the target sequence for siRNA design. Refer to the siRNA design principle, and compare it with the human genome sequence through the blast function of the GeneBank database to ensure that there is no homology; exclude potential siRNAs that have 8 consecutive bases at the 5' end of the aitisense chain paired with other genes; exclude any consecutive 14 bases base-pairing potential siRNAs with other genes. The design software was used for pre-evaluation and determination, and three targets with the best kinetic parameters were selected to enter the subsequent experimental process. A total of three interference sequences were synthesized for each gene, a...

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Abstract

The invention relates to the technical field of biomedicine, and particularly relates to a novel target preventing enterovirus 71 infection and applications thereof. Through adopting a human neuroblastoma cell (SH-SY5Y) as a target cell, and reducing expression of target cell host protein by adopting an RNA interference technique, a host factor effectively inhibiting EV71 infection of the human neuroblastoma cell (SH-SY5Y) is searched so as to prevent central nervous system virus infection and reducing damage to the nerve system. Experiments show that vesicle-associated membrane protein 1 (called as VAMP1 for short) plays an important role in EV71 infection of the human neuroblastoma cell (SH-SY5Y) and EV71 infection can be obviously inhibited by reducing expression of the VAMP1. The invention provides applications of the VAMP1 in preparation of medicines preventing and treating the enterovirus 71 infection.

Description

technical field [0001] The invention relates to the technical field of biomedicine, and is a new target and application for resisting enterovirus 71 infection. Background technique [0002] Enterovirus 71 (enterovirus71, EV71) belongs to the Picornaviridae Enterovirus genus Human Enterovirus A, and is one of the main pathogens that cause Hand-foot-mouth disease (HFMD). Currently, hand, foot and mouth disease is breaking out and spreading in many parts of the world, especially in the Asia-Pacific region. In my country, since the outbreak of hand, foot and mouth disease in several major provinces and cities in 2008, the number of people infected and the death rate of the disease have remained high, with more than 1 million cases and nearly 1,000 deaths reported every year. The main patients with HFMD are infants and young children under 5 years old. The clinical manifestations are fever, herpes and other symptoms in the hands, feet, buttocks and oral mucosa; a small number of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K45/00A61K31/7088A61P31/14
CPCA61K31/7088A61K45/00
Inventor 朱耐伟彭浩然朱勇喆徐庆强宋洪元戚中田
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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