The invention provides a plurality of Pichia pastoris strains capable of utilizing non methyl alcohol carbon source induced AOX1 promoter to express outside source protein. PpHXT1 gene in the Pichia pastoris strain mutates, thus protein can not be coded or the coded protein has no activity, and autophagy gene PpATG30 gene also mutates, thus protein can not be coded or the coded protein has no activity; or PpMIG1 gene in the Pichia pastoris strain mutates, thus protein can not be coded or the coded protein has no activity, and PpMIG2 gene in the Pichia pastoris strain mutates, thus protein can not be coded or the coded protein has no activity. The Pichia pastoris strain of the invention not only has the advantages of the existing Pichia pastoris strain but also can utilize non methyl alcohol carbon source induced AOX1 promoter to express outside source protein, is safe and environmentally friendly, has simple operation and lower cost, also can be applied to production of food or additive and is applicable to large-scale popularization and application.