Automatic dewaxing antigen retrieval system

Abstract

The invention discloses an automatic dewaxing antigen retrieval system, which comprises a reaction device, a dewaxing reagent injection device, a first reagent heating device, an eluent injection device, an enzyme diluent injection device, a concentrated enzyme reagent injection device, a second reagent heating device, a permeation agent injection device, a third reagent heating device, a gas injection device, an anhydrous ethanol injection device, a deionized water injection device and a waste liquid collection device, wherein the reaction device comprises a reaction box, a fan and a reaction heater, and various injection devices are respectively connected to the reaction box and supply various reagents and the reaction pressure required by the dewaxing reaction in the reaction box. According to the present invention, the automatic dewaxing antigen retrieval system has advantages of simple structure, convenient operation, stable performance and low dewaxing cost, and can rapidly perform dewaxing treatment on tissue slices.

Description

technical field [0001] The invention relates to the technical field of pathological tissue sections, in particular to an automatic dewaxing antigen retrieval system. Background technique [0002] Paraffin section is the most widely used method in routine histological preparation techniques. In pathological technical work, whether the paraffin sections are subjected to conventional HE staining or other special staining, it is necessary to remove the paraffin of the paraffin sections, that is, dewaxing. The dewaxing method of paraffin sections is generally to treat the paraffin sections with different reagents in a specific order, and the treatment process usually involves reagent concentrations and temperatures. [0003] At present, there are three main methods for dewaxing paraffin sections: [0004] 1. Manual method: Chinese patent CN 1975367A proposes a method for dewaxing tissue paraffin sections, which involves various reagents and reaction vessels, and involves the co...

AI Technical Summary

Problems solved by technology

Its shortcomings and shortcomings are: (1) real-time monitoring is required by professionals, and the steps are cumbersome, inefficient, and high labor costs are required; (2) some reagents in the dewaxing reaction process not only pollute the environment but are also harmful to the human body ; (3) The reaction vessel and the hanging basket are generally general-purpose, and a large amount of reagents will be wasted in the process of processing, further increasing the cost

[0005] 2. Mechanical arm method: Chinese patent CN261023Y proposes a multifunctional pathological slice processor. This method uses a mechanical boom, which drives the movement of a hanging basket equipped with tissue paraffin sections, and puts the hanging basket into each reaction section in turn. The disadvantages and shortcomings of the reaction in the container are: (1) the problem of reagent waste caused by multiple reaction containers cannot be solved; (2) the mechanical arm involves complex mechanical structures and control algorithms, and the cost is high and the system stability is relatively low. Low

Its disadvantages and shortcomings are: (1) the use of vacuum and positive pressure to provide power for liquid movement requires high air tightness of the system and poor system stability; (2) the use of vacuum and positive pressure The power to provide liquid movement cannot accurately quantitatively control the reagents, and there is still a large waste of reagents; 3) Use the method of vacuuming and applying positive pressure to provide power for liquid movement, and the process is slow when switching between positive pressure and negative pressure. Less efficient and noisy operation of positive pressure and vacuum pumps

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