Immunohistochemical staining machine and immunohistochemical staining method

Abstract

The invention belongs to the technical field of immunohistochemical staining. An instant preparation and instant use module is arranged on an immunohistochemical staining machine to achieve the instant preparation and instant use of a reagent required in the staining process of a tissue slide. After the tissue slide is stained, a cooling structure is arranged under a staining working platform andused for cooling the staining working platform. Meanwhile, an antigen retrieval module is arranged in the machine and can conduct high-temperature water bath antigen retrieval on a tissue slice.

Description

technical field [0001] The invention belongs to the technical field of immunohistochemical staining, in particular to an immunohistochemical staining machine. Background technique [0002] At present, the fully automatic immunohistochemical staining machines used in the market do not have an antigen retrieval pool structure for high temperature water bath antigen retrieval of pathological tissue sections. Therefore, the current antigen retrieval pool is equipped with an additional pressure cooker in the department, and then performs antigen retrieval in a high-temperature water bath for pathological tissue sections inside. [0003] At the same time, in the process of staining tissue slides in the existing automatic immunohistochemical staining, the reagents need to be manually prepared in advance and placed in a fixed position, so it is impossible to achieve real-time use and ready-to-use in terms of time. Reagents must be installed and placed in the reagent position before...

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Problems solved by technology

[0003] At the same time, in the process of staining tissue slides in the existing fully automatic immunohistochemical staining, the reagents need to be manually prepared in advance and placed in a fixed position, so it is impossible to achieve real-time preparation in terms of time.

Reagents must be installed and placed in the reagent position before the equipment is turned on and used. If the reagents are used in the later stages of the entire process, the actual waiting time for the currently used reagents will be too long, which will affect the particularly sensitive reagents. great

[0004] Moreover, after the staining of the tissue slides, the dyeing workbench needs to be cooled when entering the next step of the process. However, based on the fact that the current dyeing workbench does not have a cooling structure, it takes a long time to cool down during the dyeing process, resulting in a longer time for the entire process. too long

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