166results about How to "Fluorescent signal enhancement" patented technology

A method for assaying a sample for a nucleic acid damaging activity using at least one singular double-stranded nucleic acid with at least one electrochemiluminescent label, and a method for measuring an inhibitor of a nucleic acid damaging activity with at least one singular double-stranded nucleic acid using at least one electrochemiluminescent label, are disclosed.

Improved methods, systems and apparatus relating to wide field fluorescence and reflectance imaging are provided, including improved methods, systems and apparatus relating to removal of background signals such as autofluorescence and / or fluorophore emission cross-talk; distance compensation of fluorescent signals; and co-registration of multiple signals emitted from three dimensional tissues.

The present invention provides binding assays, referred to here as fluorescence proximity assays or FPA. The inventions detect binding of target molecules in a sample to a molecular probe or probes that specifically bind or hybridize to those molecules. In particular, the molecular probes are immobilized to a bead or particle, such as colloidal gold, the reflects fluorescent energy from a fluorophore. The derivatized beads are contacted to a sample of fluorescently labeled target molecules, and binding of the target is indicated by an increase in the fluorescent signal. Kits are also provided that contain materials and reagents to performing a fluorescence proximity assay.

The present invention provides processes for measuring DNA or RNA binding proteins, specific nucleic acids, as well as enzyme activities using labeled nucleic acids of labeled protein / peptide molecules.

The present invention refers to nanoparticles having optically fluorescent activity. In more detail, the invention refers to a nanoparticle matrix comprising a co-aggregate of at least one charged polyelectrolyte and at least one oppositely charged active agent, wherein the active agent is a hydrophilic optically fluorescent agent, and the invention further refers to a nanoparticle comprising said nanoparticle matrix. Optionally, the nanoparticle is surface modified. The invention also refers to a method for preparing said nanoparticle, and to a method of surface modification. Furthermore, the invention refers to uses of said nanoparticle in vitro and in vivo, and to methods for in vitro and in vivo diagnosis.

An apparatus and method for making quantitative measurements of the amounts of optical brighteners in water samples using fluorescence measurements at multiple wavelengths. First and second emission wavelength raw measurements are corrected for sample absorption to provide absorption-corrected first emission wavelength and second emission wavelength fluorescence emission values. The absorption-corrected first emission wavelength value is compared to the absorption-corrected second emission wavelength value and to similarly-determined and similarly absorption-corrected emission wavelength values or a ratio thereof obtained from a comparison water sample in which optical brighteners are not present or are only minimally present, to provide a quantitative optical brightener measurement. The apparatus and method may be field-based or laboratory-based, and may operate on a flow-through basis or on discrete samples.

The invention relates to a method for detecting ochratoxin A. The method utilizes a nucleic acid aptamer sequence fixed on a photonic crystal microsphere and a fluorescence labeled complementary sequence thereof for hybridization; after addition of a sample to be detected, a chip scanner is employed to detect fluorescence signals before and after addition of the sample; and change value of the measured fluorescence signal is used for detection of ochratoxin A in the sample. The method has detection limit of 0.001 ng / mL on ochratoxin A, linear detection range from 0.001 to 1 ng / mL; and only 2 muL of specimen is required.

A composition comprising a hydrogel particle and a fluorophore; wherein said composition produces an enhanced fluorescent signal when excited by an energy source capable of exciting the fluorophore.

The invention discloses a fluorescent reagent for detecting specificity of aluminium ions based on an aggregation-induced emission principle. The fluorescent reagent is represented as sodium4-(2, 5-diphenyl-1H-pyrrole-1-yl)benzoate (TriPP-COONa). The fluorescent reagent has fluorescence enhancement specific response to the aluminium ions, has the detection limit of 1 mu M (27 mu g / L) which is lower than the aluminium ion concentration (no higher than 200 mu g / L) in drinking water stipulated by the World Health Organization, is free of the interference of other ions, such as K+, Mg2+, Ca2+, Ba2+, Ni2+, and has short response time. The fluorescence intensity of the fluorescent reagent has good linear relationship within the aluminium ion concentration range of 1 mu M to 11 mu M. According to the linear equation in the invention, the quantitive detection of the concentration of aluminium ions in the water sample can be realized conveniently and rapidly.

There is disposed, on a substrate 41 holding a sample to be detected, a dielectric multilayer 42 which reflects excitation light e1 supplied from above the substrate 41 and transmits fluorescence f1 emitted from the sample, and the excitation light e1 is reflected at the dielectric multilayer 42 while the transmitted fluorescence f1 is detected by a light receiving unit 44, thereby providing a fluorescence measurement apparatus which can resolve a problem of reduction in detection sensitivity due to autofluorescence from the substrate or leakage of the excitation light from a light receiving filter, and which can detect the sample with high sensitivity.

The invention provides a colored super-resolution imaging device and method, and relates to the technical field of microscopic imaging. The method includes the steps: allowing a fluorescence signal of a to-be-tested sample to pass through a first dichroic mirror to reach a beam splitter to be divided into a first beam of light path and a second beam of light path; emitting the first beam of light path to a CCD camera, and collecting a super-resolution image; emitting the second beam of light path to a three-channel photomultiplier tube module, respectively collecting a red channel image, a green channel image and a blue channel image; carrying out matrix dot product of the red channel image, the green channel image and the blue channel image with the super-resolution image respectively, and respectively generating a red super-resolution image, a green super-resolution image and a blue super-resolution image; and carrying out matrix addition operation of the red super-resolution image, the green super-resolution image and the blue super-resolution image, and generating a colored super-resolution image. The problems that a current secondary scanning super-resolution microscopy technique has lower signal acquisition rate and lower efficiency are solved.

A method and composition is described for controlling the growth of fouling materials, such as scale, in aqueous systems using a tagged (fluorescent) polymer. The tagged polymer can be a fluorescent polymer which has at least one fluorescent monomeric unit derived from a fluorophore which has at least one terminal end comprising an olefinic group. The method of controlling the growth of at least one fouling material in an aqueous system can include the steps of adding the tagged polymer to the aqueous system to be treated, fluorometrically monitoring the concentration of the tagged polymer, and adjusting, as needed, the concentration of the tagged polymer and proportionally used water treatment compound or compounds effective to control the growth of at least one fouling material. The adjustment of pH before determination of the fluorescence signal can be employed to increase sensitivity of the fluorophore and minimize background interference.

The invention relates to a reactive rhodamine fluorescent probe for detecting mercury ions, and a preparation method thereof. A structural formula of the reactive rhodamine fluorescent probe RBHA is shown in the description. The preparation method comprises the steps of adding rhodamine B and hydrazine hydrate into a solvent, reacting for 1-2 h under refluxing at a temperature of 80-90 DEG C; carrying out rotary evaporation; adding water and adjusting pH; filtering; and purifying to obtain rhodamine B hydrazide; dissolving the rhodamine B hydrazide and glyoxylic acid in a solvent; reacting overnight with stirring at a room temperature; filtering; and purifying to obtain the reactive rhodamine fluorescent probe. The reactive rhodamine fluorescent probe has high selectivity for the mercury ions, has no obvious interference with other common ions, can work in an environment with a wide pH scope ranging from 5 to 10, and has application prospects in bioluminescent imaging and analysis of environmental water samples.

The white light spotlight for luminescence (e.g. biomarker) detection according to the invention comprises: •at least one solid state light emitting element to emit primary light, and •a plurality of wavelength converting materials, to convert part of primary light into secondary light, provided as an array of independent wavelength converting domains, wherein different domains comprise converting materials producing different secondary light emission bands / peaks, each converting material being adapted to convert primary light into secondary light, wherein each converting material is adapted to contribute to the total emission spectrum with at least one emission band / peak and the total secondary light emission provided by said converting materials provides a broad band emission spectrum except for at least one defined narrow wavelength range in which the spotlight produces no or significantly reduced light emission, and wherein the combined light emission from the converting materials is controllable to form said spectrum.

The present invention provides a fluorescence-based fiber optic temperature sensing system employing a probe with a hemispherically shaped phosphor attached thereto. The probe set forth herein not only produces fluorescence efficiently, but is also easily fabricated.

The invention discloses a preparation of a detection chip, comprising the steps of: 1) designing oligonucleotide probe of bacteria; 2) preparing the chip. The invention further discloses a method for detecting pathogene in multiple food samples with the detection chip, comprising the following steps: 1) designing a primer; 2) extracting DNA from samples to be detected, and getting extracting solution; 3) multi-PCR amplifying of target gene; 4) hybridization detecting of chip; and 5) detecting and analyzing of hybridization result. The detection step of the invention is simple, easy to operate, and produces high correct rate result.

This present disclosure provides methods and systems for measuring the concentration of multiple nucleic acid sequences in a sample. The nucleic acid sequences in the sample are simultaneously amplified, for example, using polymerase chain reaction (PCR) in the presence of an array of nucleic acid probes. The amount of amplicon corresponding to the multiple nucleic acid sequences can be measured in real-time during or after each cycle using a real-time microarray. The measured amount of amplicon produced can be used to determine the original amount of the nucleic acid sequences in the sample. Also provided herein are biosensor arrays, systems and methods for affinity based assays that are able to simultaneously obtain high quality measurements of the binding characteristics of multiple analytes, and that are able to determine the amounts of those analytes in solution. The present disclosure also provides a fully integrated bioarray for detecting real-time characteristics of affinity based assays.

The invention discloses a fluorescent reagent for detecting trace serum albumin, and a preparation and application of the fluorescent reagent, and belongs to the field of fluorescent bio-sensors. The fluorescent reagent is prepared from DP-TPPNa and a good solvent. The preparation method comprises the following steps: dissolving 3,4-dibromo-1,2,5-tri((methoxycarbonyl)phenyl) pyrrole in the good solvent, adding phenylboronic acid, a catalyst and basic salt, and reacting to obtain DP-TPP; dissolving the DP-TPP in a solvent, adding alkali, and reacting to obtain DP-TPPNa; and dissolving the DP-TPPNa in the good solvent to obtain the fluorescent reagent. The fluorescent reagent is capable of quantitatively detecting the serum albumin in serum, is simple in preparation method, convenient to operate and quick in response, has high sensitivity and high selectivity, is capable of producing an excellent visible detection signal, can completely get rid of the dependence on high-precision instruments according to actual requirements, and can meet the requirement of people on detecting the serum albumin in serum today.

The present invention is related to accurate detection methods for the measurement only of myelopexidase (MPO) levels or neutrophils, preferably equine neutrophils, in complex biological samples. The present invention is further related to ELISA and SIEFED assays for such detection. SIEFED detection sensitivity of active peroxidase activity was found to be enhanced by the addition of nitrite. Such MPO measurement finds its use in many applications such as the prediction, diagnosis and / or monitoring of pathologies correlated with neutrophil activation and / or destruction; the evaluation of drugs and / or immunomodulators; the assessment of immune responses, either natural and / or after treatment with immunomodulators and / or drugs; and the study of cells and their ability to fight microorganisms and / or to destroy them.

The invention discloses a fluorescent reagent for detecting trace gamma-globulin, as well as a preparation method and application thereof, and belongs to the field of fluorescent bio-sensors. The fluorescent reagent is prepared from TABD-Py-PF6 and a good solvent. The preparation method comprises the following steps: dissolving (1Z,3Z)-1,4-di(4-methoxycarbonyl) phenyl-1,4-dibromo-1,3-butadiene in the good solvent, adding 4-pyridine phenylboronic acid, a catalyst and a basic salt, and reacting to obtain TABD-Py; dissolving the TABD-Py in a solvent, adding iodomethane to synthesize iodate, adding potassium hexafluorophosphate, and reacting to obtain TABD-Py-PF6; dissolving the TABD-Py-PF6 in the good solvent to obtain the fluorescent reagent. The fluorescent reagent does not need participation of metal ions, has a specific lighting-type fluorescent response on gamma-globulin, has high and quick sensitivity on gamma-globulin detection, and has high selectivity. The fluorescent reagent has an excellent visible detection signal, can be completely separated from high-precision instruments according to actual requirement, and can meet current requirement for detecting the gamma-globulin in serum to a great extent.

The invention relates to the field of fluorescent quantitative detection and provides a high-sensitivity nano composite probe for the fluorescent quantitative detection of a blood serum tumor marker.The nano composite probe comprises gold nanoparticles loaded with a blood serum tumor marker secondary antibody to be detected and a DNA (Deoxyribonucleic Acid) enzyme chain. The nano composite probeprovided by the invention takes the gold nanoparticles as carriers and a compound formed by loading deoxyribozyme with a high catalytic property keeps the high catalytic property and recognition capability of the deoxyribozyme, and also introduces a signal transduction function of a nano-material, so that recognition and signal transduction functions are integrated. The sensitivity and specificityof the fluorescent quantitative detection are remarkably improved by combining an efficient specific recognition function of the tumor marker, high recognition and catalytic properties of the deoxyribozyme, and signal transduction and fluorescent amplification functions of the gold nanoparticles; the detections sensitivity can reach 0.01ng / mL.

The invention discloses a microfluidic chip based on Morpho menelaus and a manufacturing method thereof. The microfluidic chip utilizes wings of Morpho menelaus as bases and through local hydrophilictreatment on the wing surfaces of Morpho menelaus, a hydrophilic channel is formed. The microfluidic chip comprises a test sample loading zone, a fluorescent-labeled antibody loading zone, a capture antibody loading zone and a waste liquid zone and the above zones are orderly communicated. The microfluidic chip combines a biological source ordered structure and a self-assembled photonic crystal layer to form a heterostructure, enhances the fluorescent signal of the marker, is used for improving the sensitivity of the immunoassay and can simultaneously detect a plurality of biomarkers. The manufacturing method has simple processes, utilizes raw materials with a wide source, realizes rapid multi-component detection at the same time, promotes the application of the ordered structure in the POCT technology and can be widely applied in the fields of biological analysis and clinical detection.

The invention relates to a fluorescence spectra type rapid detection method of a phthalate substance in a paper packing material. The method comprises the following specific steps: 1) preparing phthalate substance as a standard substance; dissolving the standard substance with a solvent to obtain standard solutions with different concentration; dehydrating the standard solutions through a dehydrating agent to obtain standard solutions to be detected; scanning emission spectra of the standard solutions to be detected through a fluorescence spectra analyzer; and creating a standard curve; and 2)processing the paper packing material through the solvent to obtain extracting liquid; dehydrating the extracting liquid through the dehydrating agent to obtain extracting liquid to be detected; scanning the emission spectra of the extracting liquid to be detected through the fluorescence spectra analyzer; and then quantitatively analyzing the fluorescence signal intensity by an external standardmethod. The method has the advantages of being simple and convenient to operate, high in sensitivity, high in interference resistance, quick to detect, green, environmentally friendly, and suitable for rapid screening of massive samples.

The invention discloses a molecular probe for dual-mode imaging detection of neutrophil elastase as well as a preparation method and application of the molecular probe. The molecular probe is LET-8, and the structural formula of the LET-8 is as follows: firstly preparing a heptamethine cyanine dye (Cy7-Cl), then reacting the Cy7-Cl with 3-nitrophenol to prepare an intermediate product (Cy7-NO2), performing a reduction reaction to obtain a novel hemicyanine dye (HCyNH2), and then reacting the HCyNH2 with pentafluoropropionic anhydride to prepare the molecular probe (LET-8). After the molecular probe LET-8 reacts with HNE, a fluorescence signal and a photoacoustic signal are greatly enhanced, so that specific detection of the HNE is realized. The probe disclosed by the invention is simple in detection mechanism, high in sensitivity and strong in specificity, and has a wide application prospect.

The invention relates to the technical field of fluorescent sensing material preparation and biodetection, and particularly relates to a fluorescent probe simultaneously detecting ONOO<-> and H2S, anda synthesis method and an application thereof. The probe molecule has two reaction sites, one can be subjected to nucleophilic addition reaction with H2S, excitation wavelength being 480 nm and emitting wavelength being 580 nm, thus significantly enhancing a fluorescent signal; the other can be subjected to nucleophilic reaction with ONOO<->, excitation wavelength being 400 nm and emitting wavelength being 520 nm, then a rearrangement reaction is carried out to obtain two coumarin derivative products, the fluorescent signal, after the reactions, being significantly enhanced too. Therefore, RC-Br can detect the ONOO<-> and H2S in a double-excitation / double-emission manner. The fluorescent probe has high sensitivity and responding speed, can be applied to cell imaging well, and may be an effective analytic tool for researching interaction relationship of the ONOO<-> and H2S in a biology system.

The invention provides a novel coronavirus nucleic acid detection kit. The novel coronavirus nucleic acid detection kit comprises two crDNAs or two crRNAs. The crDNAs comprise a crDNA of a targeting Egene and a crDNA of a targeting N gene, wherein the sequence of the crDNA of the targeting E gene is as shown in any one of SEQ ID NO.3-5, the sequence of the crDNA of the targeted N gene is shown asany one of SEQ ID NO.6-8. The crRNAs comprise a crRNA of the targeting E gene and a crRNA of the targeting N gene, wherein the sequence of the crRNA of the targeting E gene is as shown in any one ofSEQ ID NO.9-11, and the sequence of the crRNA of the targeting N gene is as shown in any one of SEQ ID NO.12-14. The crRNAs related to the novel coronavirus E gene and the novel coronavirus N gene aredesigned, and screening detection is carried out in combination with a CRISPR-cpf1 system. The method has the advantages of being simple, high in speed, high in sensitivity, high in specificity and extremely high in practicability.

The invention discloses a fluorescence enhanced chip based on an all-medium artificial microstructure super-surface. An upper layer of the chip is composed of periodically-arranged nano-medium columnclusters, and a lower layer is a medium substrate; the refractive index of the upper layer is greater than that of the lower layer; the collective magnetic dipole resonance wavelength and the collective electric dipole resonance wavelength of the chip satisfy that one is equal to the excitation wavelength of a fluorescent marker, and the other is equal to the radiation wavelength of the fluorescent marker. In the periodically-arranged nano-medium column clusters, each of periodic units is a square or a regular hexagon of the same size; four nano-medium columns of the same size are contained ineach of the periodic units. The fluorescence enhanced chip disclosed by the invention has the benefits that a fluorescence signal is simultaneously enhanced from three aspects of enhancing an excitation light local field, improving the radiation quantum efficiency and improving the fluorescence collection efficiency, so that the detection sensitivity of the fluorescent marker is greatly improved.The whole chip is arranged on a low-refractive index substrate and has the advantages that the material loss is low, the preparation is simple, and the structure size can be changed to work in different wavebands.