Belonging to the technical field of metal detection, the invention discloses a high salt tolerant sensor based on functional nucleic acid of zinc and application thereof. The high salt tolerant sensorcomprises a molecular recognition element, a signal amplification element, and a signal conversion element. The molecular recognition element consists of zinc ion deoxyribozyme, the zinc ion deoxyribozyme consists of a substrate chain and an enzyme chain. The signal amplification element includes an isothermal amplification system and hemin. The isothermal amplification system includes an amplification template. The signal conversion element comprises a color developing agent. The sensor provided by the invention is constructed based on zinc ion deoxyribozyme, isothermal index amplification reaction and G-quadruplex liquid phase sensing technology, can be used for field detection of zinc ions in the environment, and has the advantages of simplicity and rapidity, low cost, high salt tolerance, high sensitivity and good selectivity, and especially can realize visual detection.