Chromium-based functional nucleic acid sensor and application thereof

Abstract

The invention discloses a chromium-based functional nucleic acid sensor and an application thereof in the technical field of metal ion detection. The sensor comprises a molecule recognition element, asignal amplification element and a signal conversion element, wherein the molecule recognition element comprises chromium ion deoxyribozyme consisting of a substrate chain and an enzyme chain; the signal amplification element comprises an isothermal amplification system and hemin, and the isothermal amplification system comprises an amplification template; the signal conversion element comprisesa color developing agent. The sensor is based on the chromium ion deoxyribozyme, an isothermal index amplification reaction and a G-quadruplex liquid phase sensing technology, is convenient, quick, high in sensitivity, high in specificity and resistant to high salinity in chromium ion detection and can be used in field detection of chromium ions in the environment.

Description

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AI Technical Summary

Benefits of technology

This patented technology uses specialized molecules called histone degrading agents that target certain types of metal atoms like iron or copper during their manufacturing process. These chemical substances help remove impurities from metals while still being able to perform various functions such as making them more durable over time. By measuring these properties accurately, researchers have been able to better identify environmental pollution caused by heavy metallization.

Problems solved by technology

Technologies currently exist but there still remains challenged because they require expensive materials like chrome plates, platins, chlorides, sulfur dioxide gas, hydrogen cyanobutrification agents, formaldehydes, nitrochromia, phosphorus pentoxytrisulfonamine, manganes, tetraazacycloheptane, trichlene, trimethylarsone, pentaerythrite, zinc oxidizers, copper powders, iron particles, urances, silica gel, cervices, rubber, reservoir shellfish, seawebies, pesticids, polytungstates, chromite flakes, chromated oils, heavy metals, nickel, caffein, vanadium, selenium, cadmium, tin, tantaluminum, ruthenium, rhodochrome, porphyllites, and similar substances found naturally occurring throughout nature worldwide. These contaminations pose potential hazards threatening humans through their ability to ingest them during consumption. There is a technical problem addressed in this patents where identifying and quantitative analysis techniques capable of accurately measuring minute quantities of chromatochlochrome without causing issues associated with current analyte-based tests.

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