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Colored super-resolution imaging device and method

A super-resolution imaging and super-resolution image technology, applied in the field of color super-resolution imaging devices, can solve the problems of low signal acquisition rate and efficiency, and achieve the effect of fast imaging speed

Active Publication Date: 2015-12-09
SHENZHEN INST OF ADVANCED TECH
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Problems solved by technology

[0006] Embodiments of the present invention provide a color super-resolution imaging device and method to solve the problem of low signal acquisition rate and efficiency in the current secondary scanning super-resolution microscopy technology

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  • Colored super-resolution imaging device and method

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Embodiment Construction

[0042] The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative work shall fall within the protection scope of the present invention.

[0043] Such as figure 2 As shown, the embodiment of the present invention provides a color super-resolution imaging device for microscopic imaging of a sample 8 to be tested, and the color super-resolution imaging device includes:

[0044] The laser transmitter 1, the first dichroic mirror 3, the beam splitter 13, the CCD camera 16, and the three-channel photomultiplier tube module 25.

[0045] The laser transmitter 1 emits excitation light...

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Abstract

The invention provides a colored super-resolution imaging device and method, and relates to the technical field of microscopic imaging. The method includes the steps: allowing a fluorescence signal of a to-be-tested sample to pass through a first dichroic mirror to reach a beam splitter to be divided into a first beam of light path and a second beam of light path; emitting the first beam of light path to a CCD camera, and collecting a super-resolution image; emitting the second beam of light path to a three-channel photomultiplier tube module, respectively collecting a red channel image, a green channel image and a blue channel image; carrying out matrix dot product of the red channel image, the green channel image and the blue channel image with the super-resolution image respectively, and respectively generating a red super-resolution image, a green super-resolution image and a blue super-resolution image; and carrying out matrix addition operation of the red super-resolution image, the green super-resolution image and the blue super-resolution image, and generating a colored super-resolution image. The problems that a current secondary scanning super-resolution microscopy technique has lower signal acquisition rate and lower efficiency are solved.

Description

Technical field [0001] The present invention relates to the field of microscopic imaging technology, in particular to a color super-resolution imaging device and method. Background technique [0002] Currently, in structured light illumination technology, in order to achieve super-resolution microscopic imaging, it is necessary to perform multiple image acquisitions of the sample to be tested, and perform a complex image reconstruction process after the acquisition to restore the super-resolution image of the sample to be tested. It can be seen that the traditional super-resolution microscopy imaging method is not only time-consuming, but multiple collections will further increase the probability of sample photobleaching. [0003] At present, a secondary scanning super-resolution microscopy technology has emerged, which is a new type of structured light illumination super-resolution microscopy technology. Its basic principles can be as follows figure 1 Shown. Among them, there are...

Claims

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Application Information

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IPC IPC(8): G01N21/64
Inventor 郑炜陈龙超
Owner SHENZHEN INST OF ADVANCED TECH
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